Albumin multiplier in kidney vasa recta analyzed by microspectrophotometry of T-1824

Ws, Wilde; Vorburger, C

doi:10.1152/ajplegacy.1967.213.5.1233

Cited by 27 publications

(7 citation statements)

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“…3) suggests that colloid oncotic pressure may have been lower in the peritubular capillaries of the BUL rats than in the controls. Although arterial plasma protein concentrations were not measured, there was no significant difference in arterial hematocrits between the BUL and control rats (42.8 ±1.3% vs. 44.0 ±2.1%) suggesting that no major change had occurred in intravascular volume in the BUL animals. Decreased colloid oncotic pressure has been shown to reduce net sodium reabsorption, at least in the proximal tubule (62).…”

Section: Methodsmentioning

confidence: 85%

“…In the normal medulla, the hypertonic environment probably causes blood viscosity to rise due to the crenation of red cells (41) and the threefold increase in protein concentration that occurs in the vasa recta (42)(43)(44)(45)(46). Therefore, medullary hypertonicity may determine, in part, the efferent resistance of the juxtamedullary glomneruli.…”

Section: Methodsmentioning

confidence: 99%

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A Micropuncture Study of Postobstructive Diuresis in the Rat

Yarger¹,

Aynedjian²,

Bank³

1972

J. Clin. Invest.

100

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A B S T R A C T In order to investigate the syndrome of postobstructive diuresis, clearance and micropuncture studies were carried out in rats after relief of 24 hr of bilateral (BUL) or unilateral (UUL) ureteral ligation. In rats with BUL, a striking diuresis and natriuresis occurred when the obstruction to one kidney (the experimental kidney) was relieved. The results were not influenced by administration of vasopressin or d-aldosterone. Whole kidney clearances of inulin and p-aminohippuric acid (PAH) in the experimental kidney were reduced to 10% and 20% of normal, respectively. Superficial nephron inulin and PAH clearances were also reduced, but only to 40% and 45%, respectively. These findings suggest a heterogeneity of nephron function in which deep nephrons were functioning poorly or not at all. To investigate the site of impaired tubular reabsorption in the surface nephrons, absolute and fractional water reabsorption was measured. Absolute reabsorption was found to be decreased all along the nephron. Fractional reabsorption in proximal tubules was normal, as indicated *by an average endproximal tubular fluid per plasma inulin (TF/Pi.) of 2.16 vs. 2.30 in controls. TF/Pri was markedly decreased in distal tubules (2.91 vs. 8.02) and final urine (5.56 vs. 263). These observations indicate that the major sites of impaired sodium reabsorption leading to the diuresis were beyond the proximal tubule.

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Section: Methodsmentioning

confidence: 85%

Section: Methodsmentioning

confidence: 99%

A Micropuncture Study of Postobstructive Diuresis in the Rat

Yarger¹,

Aynedjian²,

Bank³

1972

J. Clin. Invest.

100

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“…Those investigations suggested the existence ofa large extravascular pool ofalbumin in the renal medulla. Injection ofalbumin labeled with various markers including radioiodine (3), FITC (4,5), and Evans blue dye (5,6) supported the presence of interstitial albumin. Histochemical methods have been used to examine the pathway by which macromolecules gain access to the interstitium.…”

Section: Introductionmentioning

confidence: 97%

Molecular sieving of albumin by the ascending vasa recta wall.

Pallone

1992

J. Clin. Invest.

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Molecular sieving of albumin by ascending vasa recta. Evidence exists to support the presence of an extravascular pool of albumin in the renal medullary interstitium. This study used microperfusion in vivo to measure the transport of '25I-labeled albumin from descending (DVR) and ascending vasa recta (AVR) to the papillary interstitium. Perfusions were performed during furosemide diuresis with a buffer containing FITC-labeled dextran (FITC-Dx) 2 X 106 mol wt and 125I-albumin. Perfusate albumin and collection pressure were adjusted to induce either zero transcapillary volume flux (Jv) or high volume flux. When Jv was zero, the collectate-to-perfusate ratios of FITC-Dx (RDX) and 125I-albumin (Rkb) in the DVR and AVR were identical implying that diffusive efflux of albumin was immeasurably small. In contrast, when Jv was increased, paired comparison of R.Jb and RDX in the same AVR revealed a difference, 1.58±0.06 vs 1.72±0.08, respectively (P < 0.01). AVR perfusions in hydropenic animals showed similar results, R.Jb = 1.70±0.07 and RDX = 2.00±0.07 (P < 0.01).These data suggest that albumin transport across vasa recta in vivo is likely to be governed by solvent drag. The reflection coefficient of the AVR wall to 125I-albumin is estimated to be 0.78. (J. Clin. Invest. 1992. 90:30-34.) Key words:

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“…A possible connection between extravascular albumin and the urine concentrating process has been described by Pinter [19] who found a significant correlation between the urine osmolality and the extravascular albumin distribution space in the canine medulla, but no explanation for this finding was apparent. Wilde and Vorburger [33] discussed the interaction between the accumulation of extravascular albumin by means of an ‘albumin multiplier’ and the entry of Na into the inner medullary interstitium. MacPhee [16] concluded that both HA and extravasated albumin were involved in the inner medullary concentrating process, but the mechanism of this effect was not specified.…”

Section: Discussionmentioning

confidence: 99%

An Inner Medullary Concentrating Process Actuated by Renal Pelvic/Calyceal Muscle Contractions: Assessment and Hypothesis

Pinter

Shohet

2009

Nephron Physiol

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We propose a mechanism of an inner medullary concentrating process in which water extraction is accomplished by a colloid osmotic mechanism and hydrostatic pressure. There are 3 essential features of the proposal: 1. the fluid compartmental structure of the inner medullary interstitium: owing to molecular exclusion, negatively charged macromolecules, i.e. hyaluronan and extravasated plasma albumin form separate compartments (the HA and the EPA compartments); the resulting Gibbs-Donnan effect governs the movements of both ions and water. 2. NaCl, in high concentration in the inner medulla conditioned by the outer medullary countercurrent processes, significantly reduces the equilibrium colloid osmotic pressure between these compartments. 3. Urea, also accumulated here by special transport mechanisms, increases the mobility of water molecules and the flexibility of the HA fibrils by loosening hydrogen bonds. These features suggest that rhythmic, small pressure increases of the pelvic/calyceal muscles squeeze dilute fluid out of the HA compartment and, at the same time, accelerate the outflow of fluid and albumin into the ascending vasa recta from the EPA compartment. Further, they suggest a mechanism for the phenomenon that living organisms utilize hydrostatic pressure generated by muscle contractions in water economy namely, concentrating and diluting body fluids.

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Albumin multiplier in kidney vasa recta analyzed by microspectrophotometry of T-1824

Cited by 27 publications

References 0 publications

A Micropuncture Study of Postobstructive Diuresis in the Rat

A Micropuncture Study of Postobstructive Diuresis in the Rat

Molecular sieving of albumin by the ascending vasa recta wall.

An Inner Medullary Concentrating Process Actuated by Renal Pelvic/Calyceal Muscle Contractions: Assessment and Hypothesis

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