Albumin influences total plasma antioxidant capacity favorably in patients with acute lung injury*

Quinlan, Gregory J.; Mumby, Sharon; Martin, Greg S.; Bernard, Gordon R.; Gutteridge, John M.C.; Evans, Timothy W.

doi:10.1097/01.ccm.0000114574.18641.5d

Cited by 178 publications

(109 citation statements)

References 28 publications

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“…It can also function as a transporter protein for fatty acids and act as a carrier protein for progesterone as well as possessing antioxidant properties (Kragh-Hansen et al 1990, Quinlan et al 2004, Fanali et al 2012. Our 2D-DIGE analysis displayed a 2.7-fold increase in ALB in the CVF of women with subsequent PTL.…”

Section: Discussionmentioning

confidence: 81%

Prediction of spontaneous preterm labour in at-risk pregnant women

Liong¹,

Quinzio²,

Fleming³

et al. 2013

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The ability to recognise women who are at-risk of preterm labour (PTL) is often difficult. Over 50% of women who are identified with factors associated with an increased risk of preterm birth will ultimately deliver at term. The cervicovaginal fluid (CVF) comprises a range of proteins secreted by gestational tissues, making it an ideal candidate for the screening of differentially expressed proteins associated with PTL. CVF samples were collected from at-risk asymptomatic women. Two-dimensional gel electrophoresis techniques were used to examine the CVF proteome of women who spontaneously delivered preterm 11-22 days later compared with gestation-matched women who delivered at term. Five candidate biomarkers were selected for further validation in a larger independent cohort of asymptomatic women. Thioredoxin (TXN) and interleukin 1 receptor antagonist (IL1RN) concentrations in the CVF were found to be significantly reduced up to 90 days prior to spontaneous PTL compared with women who subsequently delivered at term. TXN was able to predict spontaneous PTL within 28 days after sampling with a high positive predictive value (PPV) and negative predictive value (NPV) of 75.0% and 96.4% respectively. IL1RN also showed comparable PPV and NPV of 72.7% and 95.7% respectively. The discovery of these differentially expressed proteins may assist in the development of a new predictive bedside test in identifying asymptomatic women who have an increased risk of spontaneous PTL.

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Section: Discussionmentioning

confidence: 81%

Prediction of spontaneous preterm labour in at-risk pregnant women

Liong¹,

Quinzio²,

Fleming³

et al. 2013

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“…The most frequently oxidized proteins were albumin and SP-A. These proteins have antioxidative properties, [26][27][28] and thus may take part in the first-row defense against free-radical attack to protect the mucosa from excessive damage. [29][30][31] Another frequently oxidized protein was surfactant protein A (SP-A).…”

Section: Oxidation Pattern Of Bal Proteinsmentioning

confidence: 99%

Protein Oxidation by Chronic Pulmonary Diseases in Children

Starosta

Griese

2005

Pediatric Pulmonology

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Summary. The oxidation of proteins may play an important role in the pathogenesis of chronic inflammatory lung diseases, and may contribute to lung damage. However, the extent of oxidation and the distribution among proteins are not known for most pediatric lung diseases. In this work, protein oxidation was assessed as protein carbonyls. Bronchoalveolar lavages (BAL) from children with chronic lung diseases were investigated by dot-blot assay for content and for pattern of distribution of oxidized proteins by two-dimensional (2D) electrophoresis and Western blotting. Significantly higher levels of protein oxidation than in healthy controls were determined in groups of patients with interstitial lung disease, gastro-esophageal reflux disease, and pulmonary alveolar proteinosis. The proteins most sensitive to oxidation were serum albumin, surfactant protein A, and a1-antitrypsin. Our data show increased oxidative stress in lungs of children with chronic pulmonary diseases, with significant interindividual variations. The extent of protein oxidation was proportional to the count of neutrophilic granulocytes in BAL fluid. These findings strongly support the concept that an abundance of reactive oxygen species produced during neutrophilic inflammation may be a deleterious factor, leading to pulmonary damage in these patients.

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“…(1,(10)(11)(12) The chemical structure of serum albumin includes a thiol group that contains a sulfhydryl radical (-SH) with antioxidant properties. (13) It is responsible for 80% of thiol radicals in the circulating blood. Additionally, nitric oxide (NO) may bind to albumin, forming S-nitrous-albumin and regulating its plasma levels.…”

Section: In Vitro Effectsmentioning

confidence: 99%

“…Albumin's antioxidant effects have previously been demonstrated in the context of acute lung injury. (13) Albumin may also act as an antioxidant by binding to bilirubin, a molecule that generates a heme radical when metabolized; heme has proinflammatory effects in conditions such as sepsis. (15) Albumin's antioxidant properties are provided by the cisteine-34 radical that binds to NO, its high affinity and low specificity binding sites for bilirubin and heme, and the N-terminal radical that binds copper, chromium and nickel).…”

Section: In Vitro Effectsmentioning

confidence: 99%