Albumin-driven disassembly of lipidic nanoparticles: the specific case of the squalene-adenosine nanodrug

Gobeaux, Frédéric; Bizeau, Joëlle; Samson, Firmin; Marichal, Laurent; Grillo, Isabelle; Wien, Frank; Yesylevsky, Semen O; Ramseyer, Christophe; Rouquette, Marie; Lepêtre-Mouelhi, Sinda; Desmaële, Didier; Couvreur, Patrick; Guénoun, P.; Renault, Jean-Philippe; Testard, Fabienne

doi:10.1039/c9nr06485k

Cited by 10 publications

(17 citation statements)

References 83 publications

(104 reference statements)

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“…As an example, intrinsic fluorescence of BSA is due to two tryptophan residues, Trp-212 and Trp-134. However, the fluorescence of these residues is influenced by their environment [139]. Thus, it is tricky to use this property to quantify BSA in solution, but it is very interesting to characterize the interaction with other components.…”

Section: Iii121 Intrinsic Fluorescencementioning

confidence: 99%

“…to study the interaction of BSA with tuftsin [140], wogonin [141], glutathione [142] and more recently with lipidic NPs by applying the Stern-Volmer model [139]. It has also been used by Cacicedo et al to show the interaction between Levo and HSA, which impacts the Levo release from bacterial cellulose film [98].…”

Section: Iii121 Intrinsic Fluorescencementioning

confidence: 99%

“…It notably allows the imaging of systems in their native form in solution. Thus, it is a very interesting technique to observe systems such as lipid-based NPs [139,169], liposomes [170][171][172] or even lipoplexes [173]. For example, Colombani et al used CryoTEM to observe the impact of the composition of lipoplexes, made of cationic liposomes and DNA, and of solvent on the lipoplexes structure [173].…”

Section: Iii162 Cryotemmentioning

confidence: 99%

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Design and applications of protein delivery systems in nanomedicine and tissue engineering

Bizeau

Mertz

2021

Advances in Colloid and Interface Science

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Section: Iii121 Intrinsic Fluorescencementioning

confidence: 99%

Section: Iii121 Intrinsic Fluorescencementioning

confidence: 99%

Section: Iii162 Cryotemmentioning

confidence: 99%

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Design and applications of protein delivery systems in nanomedicine and tissue engineering

Bizeau

Mertz

2021

Advances in Colloid and Interface Science

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“…The best docking pose was kept in each simulation. The methodology and scripts for ensemble docking used in our previous works [29][30][31] were used. No side chains were treated as flexible, because the possible protein flexibility was already accounted for by means of MD simulations.…”

Section: Ensemble Docking Simulationsmentioning

confidence: 99%

“…Third, an extensive ensemble docking analysis is made on a representative set of simulation snapshots extracted from equilibrated molecular dynamics (MD) trajectories, which takes into account both the local flexibility of the binding site and the large-scale protein motions. This methodology was already used with great success for the proteins with complex internal dynamics, such as STAT1/STAT3 [28,29] and human serum albumin [30,31].…”

Section: Introductionmentioning

confidence: 99%

Membrane Environment Modulates Ligand-Binding Propensity of P2Y12 Receptor

et al. 2021

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The binding of natural ligands and synthetic drugs to the P2Y12 receptor is of great interest because of its crucial role in platelets activation and the therapy of arterial thrombosis. Up to now, all computational studies of P2Y12 concentrated on the available crystal structures, while the role of intrinsic protein dynamics and the membrane environment in the functioning of P2Y12 was not clear. In this work, we performed all-atom molecular dynamics simulations of the full-length P2Y12 receptor in three different membrane environments and in two possible conformations derived from available crystal structures. The binding of ticagrelor, its two major metabolites, adenosine diphosphate (ADP) and 2-Methylthioadenosine diphosphate (2MeS-ADP) as agonist, and ethyl 6-[4-(benzylsulfonylcarbamoyl)piperidin-1-yl]-5-cyano-2-methylpyridine-3-carboxylate (AZD1283)as antagonist were assessed systematically by means of ensemble docking. It is shown that the binding of all ligands becomes systematically stronger with the increase of the membrane rigidity. Binding of all ligands to the agonist-bound-like conformations is systematically stronger in comparison to antagonist-bound-likes ones. This is dramatically opposite to the results obtained for static crystal structures. Our results show that accounting for internal protein dynamics, strongly modulated by its lipid environment, is crucial for correct assessment of the ligand binding to P2Y12.

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A comprehensive investigation of the interactions of human serum albumin with polymeric and hybrid nanoparticles

Ural,

Joseph,

Wien

et al. 2024

Drug Deliv. and Transl. Res.

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Nanoparticles (NPs) engineered as drug delivery systems continue to make breakthroughs as they offer numerous advantages over free therapeutics. However, the poor understanding of the interplay between the NPs and biomolecules, especially blood proteins, obstructs NP translation to clinics. Nano-bio interactions determine the NPs' in vivo fate, e cacy and immunotoxicity, potentially altering protein function. To ful ll the growing need to investigate nano-bio interactions, this study provides a systematic understanding of two key aspects: i) protein corona (PC) formation and ii) NP-induced modi cations on protein's structure and stability. A methodology was developed by combining orthogonal techniques to analyze both quantitative and qualitative aspects of nano-bio interactions, using human serum albumin (HSA) as a model protein. Protein quanti cation via, liquid chromatography-mass spectrometry, and capillary zone electrophoresis (CZE) clari ed adsorbed protein quantity and stability. CZE further unveiled qualitative insights into HSA forms (native, glycated HSA and cysteinylated), while synchrotron radiation circular dichroism enabled analyzing HSA's secondary structure and thermal stability. Comparative investigations of NP cores (organic vs hybrid), and shells (with or without polyethylene glycol (PEG)) revealed pivotal factors in uencing nano-bio interactions. Polymeric NPs based on poly(lactic-co-glycolic acid) (PLGA) and hybrid NPs based on metal-organic frameworks (nanoMOFs) presented distinct HSA adsorption pro les. PLGA NPs had protein-repelling properties while inducing structural modi cations on HSA. In contrast, HSA exhibited a high a nity for nanoMOFs forming a PC altering thereby the protein structure. A shielding effect was gained through PEGylation for both types of NPs, avoiding the PC formation as well as the alteration of unbound HSA structure.

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Albumin-driven disassembly of lipidic nanoparticles: the specific case of the squalene-adenosine nanodrug

Abstract: Albumin forms a complex with the squalene-adenosine prodrug and by doing so drives the disassembly of the squalene-adenosine nanoparticles.

Cited by 10 publications

References 83 publications

Design and applications of protein delivery systems in nanomedicine and tissue engineering

Design and applications of protein delivery systems in nanomedicine and tissue engineering

Membrane Environment Modulates Ligand-Binding Propensity of P2Y12 Receptor

A comprehensive investigation of the interactions of human serum albumin with polymeric and hybrid nanoparticles

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