Albumin-based nanoparticles as contrast medium for MRI: vascular imaging, tissue and cell interactions, and pharmacokinetics of second-generation nanoparticles

Wallnöfer, E. A.; Thurner, G.; Kremser, Christian; Talasz, Heribert; Stollenwerk, Maria; Helbok, Anna; Klammsteiner, N; Albrecht-Schgoer, Karin; Dietrich, Hermann; Jaschke, Werner; Debbage, Paul

doi:10.1007/s00418-020-01919-0

Cited by 3 publications

(10 citation statements)

References 321 publications

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“…The accumulation of methanol can negatively affect cell growth and later reduce expression levels. Hence, the optimum methanol inducer concentration needs to be evaluated for each expressed protein (White et al, 1994).…”

Section: Discussionmentioning

confidence: 99%

“…The SMD1168 strain does not have vacuole peptidase A (pep4). This enzyme is responsible for the activation of carboxypeptidase Y and protease B1 (White et al, 1994). The shortcoming of the protease deficiency strain is that it has lower viability, possesses a slower growth rate, and is more difficult to handle in a shake flask and fermenter cultures.…”

Section: Discussionmentioning

confidence: 99%

“…It is most likely that only one DTPA molecule can link to HSA through Lys199. According to Wang et al (2020), this Lys residue can serve as a drug linking site. The partial conjugation possibly happens because there are multiple lysine residues that are available for linkage formation between the HSA and DTPA.…”

Section: Discussionmentioning

confidence: 99%

“…Detection using MRI usually uses gadolinium-diethylenetriamine pentaacetate (Gd-DTPA) contrast agents to obtain a cross-section of the body using magnetic fields (Meade, 2004;Na et al, 2009;Pierre et al, 2014;Zou and Lu, 2013). HSA reversibly can bind to Gd-DTPA, resulting in significantly higher relaxivity and intravascular retention than the existing contrast agent used in magnetic resonance (Wu et al, 2007;Goyen, 2008;Zhen et al, 2012). The HSA-Gd complex has been widely studied.…”

Section: Introductionmentioning

confidence: 99%

“…However, these protease-deficient cells were not as strong as the wild-type strain with respect to PEP4. These strains have lower viability and slower growth rates, are more difficult to transform, and are more difficult to handle in shake flasks and fermenter cultures (White et al, 1994). The rHSA produced furthermore was conjugated to Gd-DTPA which can later be applied as a contrast agent.…”

Section: Introductionmentioning

confidence: 99%

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Expression of human serum albumin in Pichia pastoris protease-deficient host and conjugation with gadolinium-diethylenetriamine pentaacetate for application as a contrast agent

Gaffar¹,

Anggraeni²,

Novianti³

et al. 2021

J Appl Pharm Sci

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Human serum albumin (HSA) is a plasma protein with a molecular weight of 66.5 kDa, which is most widely used in the pharmaceutical and clinical fields. One application of HSA in a drug delivery system is to increase the relaxation time on magnetic resonance imaging. The combination of contrast agent, gadolinium-diethylenetriamine pentaacetate (Gd-DTPA), with HSA can produce better imaging. This study aims to express the recombinant HSA (rHSA) in the Pichia pastoris protease-deficient host, SMD1168, purification using chromatographic techniques and conjugation with Gd-DTPA. Optimization of rHSA expression by P. pastoris was done by varying the methanol inducer, 0.75%, 1.0%, 1.25%, and 1.5%, as well as characterization by sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE). Furthermore, the rHSA was purified using gel filtration, characterization by Size Exclusion Chromatography-High Performance Liquid Chromatography (SEC-HPLC), and conjugation with Gd-DTPA. The rHSA (~66.5 kDa) was characterized from broth medium of P. pastoris SMD1168. The optimum conditions for rHSA expression were 1.5% methanol at 48 hours incubation, which resulted in 0.44 g/l of rHSA. The rHSA with 81.84% purity was obtained by gel filtration, and conjugation with Gd-DTPA produced high-purity Gd-DTPA-rHSA, demonstrated by a single peak (rt = 10.493 minutes). The proteasedeficient P. pastoris was successfully used to express intact rHSA and the rHSA was successfully conjugated to Gd-DTPA with high purity.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Expression of human serum albumin in Pichia pastoris protease-deficient host and conjugation with gadolinium-diethylenetriamine pentaacetate for application as a contrast agent

Gaffar¹,

Anggraeni²,

Novianti³

et al. 2021

J Appl Pharm Sci

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Multifunctional and stimuli-responsive liposomes in hepatocellular carcinoma diagnosis and therapy

Samaei,

Daryab,

Gholami

et al. 2024

Translational Oncology

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Advances in Nanoliposomes for the Diagnosis and Treatment of Liver Cancer

Zhang

et al. 2022

IJN

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The mortality rate of liver cancer is gradually increasing worldwide due to the increasing risk factors such as fatty liver, diabetes, and alcoholic cirrhosis. The diagnostic methods of liver cancer include ultrasound (US), computed tomography (CT), and magnetic resonance imaging (MRI), among others. The treatment of liver cancer includes surgical resection, transplantation, ablation, and chemoembolization; however, treatment still faces multiple challenges due to its insidious development, high rate of recurrence after surgical resection, and high failure rate of transplantation. The emergence of liposomes has provided new insights into the treatment of liver cancer. Due to their excellent carrier properties and maneuverability, liposomes can be used to perform a variety of functions such as aiding in imaging diagnoses, combinatorial therapies, and integrating disease diagnosis and treatment. In this paper, we further discuss such advantages.

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Albumin-based nanoparticles as contrast medium for MRI: vascular imaging, tissue and cell interactions, and pharmacokinetics of second-generation nanoparticles

Cited by 3 publications

References 321 publications

Expression of human serum albumin in Pichia pastoris protease-deficient host and conjugation with gadolinium-diethylenetriamine pentaacetate for application as a contrast agent

Expression of human serum albumin in Pichia pastoris protease-deficient host and conjugation with gadolinium-diethylenetriamine pentaacetate for application as a contrast agent

Multifunctional and stimuli-responsive liposomes in hepatocellular carcinoma diagnosis and therapy

Advances in Nanoliposomes for the Diagnosis and Treatment of Liver Cancer

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