Albumin Augmentation Improves Condition of Guinea Pig Hearts After 4 hr of Cold Ischemia

Jacob, Matthias; Paul, Oliver; Mehringer, L.; Chappell, Daniel; Rehm, Markus; Welsch, Ulrich; Kaczmarek, Ingo; Conzen, P.; Becker, Bernhard F.

doi:10.1097/tp.0b013e31819c83b5

Cited by 128 publications

(89 citation statements)

References 41 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…It has been used as a nonmetabolisable impermeate added to perfusates during post-ischemic reperfusion to reduces cell edema (i.e., cell swelling) by retaining water outside myocytes (22). In our Langendorffperfused ventricle preparations, mannitol perfusion was associated with increased V IS (Fig.…”

Section: Perfusate Effects On Myocardiummentioning

confidence: 88%

“…In contrast, mannitol is a small molecule (molecular weight, 182.17) that raised perfusate osmolarity (see METHODS) and should extravasate freely (22). It has been used as a nonmetabolisable impermeate added to perfusates during post-ischemic reperfusion to reduces cell edema (i.e., cell swelling) by retaining water outside myocytes (22).…”

Section: Perfusate Effects On Myocardiummentioning

confidence: 99%

See 1 more Smart Citation

Interstitial volume modulates the conduction velocity-gap junction relationship

Veeraraghavan

Salama

Poelzing

2012

American Journal of Physiology-Heart and Circulatory Physiology

126

View full text Add to dashboard Cite

Cardiac conduction through gap junctions is an important determinant of arrhythmia susceptibility. Yet, the relationship between degrees of Gj uncoupling and conduction velocity (θ) remains controversial. Conflicting results in similar experiments are normally attributed to experimental differences. We hypothesized that interstitial volume modulates conduction velocity and its dependence on Gj. Interstitial volume (VIS) was quantified histologically from guinea pig right ventricle. Optical mapping was used to quantify conduction velocity and anisotropy (ARθ). Albumin (4 g/l) decreased histologically assessed VIS, increased transverse θ by 71 ± 10%, and lowered ARθ. Furthermore, albumin did not change isolated cell size. Conversely, mannitol increased VIS, decreased transverse θ by 24 ± 4%, and increased ARθ. Mannitol also decreased cell width by 12%. Furthermore, mannitol was associated with spontaneous ventricular tachycardias in three of eight animals relative to zero of 15 during control. The θ-Gj relationship was assessed using the Gj uncoupler carbenoxolone (CBX). Whereas 13 μM CBX did not significantly affect θ during control, it slowed transverse θ by 38 ± 9% during mannitol (edema). These data suggest changes in VIS modulate θ, ARθ, and the θ-Gj relationship and thereby alter arrhythmia susceptibility. Therefore, VIS may underlie arrhythmia susceptibility, particularly in diseases associated with gap junction remodeling.

show abstract

Section: Perfusate Effects On Myocardiummentioning

confidence: 88%

Section: Perfusate Effects On Myocardiummentioning

confidence: 99%

Interstitial volume modulates the conduction velocity-gap junction relationship

Veeraraghavan

Salama

Poelzing

2012

American Journal of Physiology-Heart and Circulatory Physiology

126

View full text Add to dashboard Cite

show abstract

“…Albumin attenuated glycocalyx shedding during 4 h of cold ischaemia and reperfusion in guinea pig hearts, reducing interstitial oedema and intracoronary adhesion of leucocytes [40]. Albumin dilates coronary arteries compared with HES, possibly by enhancing NO production in response to shear stress: vasodilation by albumin was reduced when NO synthase was blocked and when the glycocalyx was damaged [18].…”

Section: Damage To the Endothelial Surface Layermentioning

confidence: 99%

The endothelial glycocalyx: a review of the vascular barrier

Alphonsus¹,

2014

View full text Add to dashboard Cite

SummaryThe endothelial glycocalyx is an important part of the vascular barrier. The glycocalyx is intimately linked to the homoeostatic functions of the endothelium. Damage to the glycocalyx precedes vascular pathology. In the first part of this paper, we have reviewed the structure, physiology and pathology of the endothelial glycocalyx, based on a literature search of the past five years. In the second part, we have systematically reviewed interventions to protect or repair the glycocalyx. Glycocalyx damage can be caused by hypervolaemia and hyperglycaemia and can be prevented by maintaining a physiological concentration of plasma protein, particularly albumin.

show abstract

“…95 Albumin can be used in severe sepsis and septic shock when patients require substantial amounts of crystalloids, 1 but it should not be used in patients with traumatic brain injury. 95,96 Moreover, instead of its theoretical benefits in septic patient, 97,98 the SAFE study showed no significant difference between saline and albumin in ICU patients. 96 Semisynthetic colloids as hydroxyethyl starch solutions should not be used because of the increased mortality and risk of renal replacement therapy.…”

Section: Septic Shock: Pathophysiology and Hemodynamic Resuscitationmentioning

confidence: 99%

Management of sepsis: from evidence to clinical practice

et al. 2016

View full text Add to dashboard Cite

Sepsis is one of the leading causes of death in hospitalized patients and its management involves a lot of specialist. Internist is required to demonstrate his competence since the beginning when the diagnosis is not so easy to be clarified. A rapid clinical suspicion permits a prompt management of the patient that means important mortality reduction. However, it is essential to understand the source of infection and echography represents a rapid, economic, useful and widespread tool with whom Internist should become more and more confident. The following review is a practical guide to manage septic patients according to the most recent literature, underlining aspects of antibiotic therapy, hemodynamic stabilization and supportive therapy. To limit sepsis mortality, a valid Internist should be culturally prepared and especially able to cooperate with other specialists, because a strong enemy requires a strong team.

show abstract

Albumin Augmentation Improves Condition of Guinea Pig Hearts After 4 hr of Cold Ischemia

Abstract: Augmenting HTK with human albumin improves endothelial integrity and heart performance after 4 hr cold ischemia, because of a marked protection of the endothelial glycocalyx. For the prevention of acute and chronic graft failure, the glycocalyx might represent a new target.

Cited by 128 publications

References 41 publications

Interstitial volume modulates the conduction velocity-gap junction relationship

Interstitial volume modulates the conduction velocity-gap junction relationship

The endothelial glycocalyx: a review of the vascular barrier

Management of sepsis: from evidence to clinical practice

Contact Info

Product

Resources

About