Albatross/FBF1 contributes to both centriole duplication and centrosome separation

Abstract

The centrosome is a small but important organelle that participates in centriole duplication, spindle formation, and ciliogenesis. Each event is regulated by key enzymatic reactions, but how these processes are integrated remains unknown. Recent studies have reported that ciliogenesis is controlled by distal appendage proteins such as FBF1, also known as Albatross. However, the precise role of Albatross in the centrosome cycle, including centriole duplication and centrosome separation, remains to be determined. Here, we report a novel function for Albatross at the proximal ends of centrioles. Using Albatross monospecific antibodies, full‐length constructs, and siRNAs for rescue experiments, we found that Albatross mediates centriole duplication by recruiting HsSAS‐6, a cartwheel protein of centrioles. Moreover, Albatross participates in centrosome separation during mitosis by recruiting Plk1 to residue S348 of Albatross after its phosphorylation. Taken together, our results show that Albatross is a novel protein that spatiotemporally integrates different aspects of centrosome function, namely ciliogenesis, centriole duplication, and centrosome separation.

<strong> </strong>Spatial and Functional Mapping of Spliceosome and Centrosome Proximity Revealed by Proteogenomics Profiling<strong> </strong>

“…was highly and selectively interconnected with a variety of splicing genes some of which found to be localized in human centrosomes (Figure 4B). These results are in line with recent studies showing that FBF1 and OFD1 could have a key role in modulating the translation of specific transcripts nearby centrosomes or integrating different centrosomal functions such as ciliogenesis and cell-cycle progression [14,49]. To further clarify the possible genetic co-regulation, we used a public RNAseq dataset from human immortalized cells induced in spontaneous or CDK4/6 inhibitor-induced quiescence [28].…”

Nek2 kinase displaces distal appendages from the mother centriole prior to mitosis

“…This suggests that either residual levels of LRRC45 are sufficient to keep FBF1 at mitotic centrioles or FBF1 might be recruited to mitotic centrioles in an LRRC45-independent manner. Indeed, FBF1 was recently reported to localize at the proximal ends of the centriole in RPE1 and HeLa cells ( Inoko et al, 2018 ). Super-resolution microscopy also indicated that FBF1 localization differs from that of other DAs as FBF1 forms bladelike structures that intercalate with DAs ( Yang et al, 2018 ).…”