Alarm Test: A Novel Chemical-Free Behavioural Assessment Tool for Zebrafish

Abstract

Zebrafish (ZF) is an incredible animal for the study of neurological disorders. Its behaviour is like higher vertebrate animals, which makes it gainful and robust. Understanding the psychological and biological implications of housing settings for ZFs is very crucial in improving the replicability and dependability of ZF behavioural research. Individual housing triggers depression-like symptoms that suggest that housing conditions have negative effects on ZF and can result in the data discrepancy. Based on various behavioural analyses, we have evaluated that the ZFs kept in isolation and the ZFs kept in herd conditions exhibit different behavioural patterns. Interestingly, normal isolated subjects exhibit similar behavioural patterns as Alzheimer disease (AD)-induced subjects; hence, this can have serious implications on any study concerning behaviour of ZFs. Therefore, we have reported a new behavioural test named "Alarm Test", which effectively discriminates normal isolated subjects from AD subjects. Alarm Test is observed to be better than other tests used for studying fear and anxiety in ZFs as it uses the indigenous compound released by ZFs during fear and makes use of the same for analysis. This can reduce the involvement of chemicals during behavioural analysis as well as sacrifice of ZFs for collection of alarm substance.

Evaluation of toxicity and teratogenicity of anti-psychotic drugs followed by determination of anti-psychotic property of Mangifera indica bark through behavioral assay on Danio rerio model

“…For analysis and recording, we utilized a videorecording camera. Before beginning the test, it must be guaranteed that the water is at the optimum temperature (Akhmola-Mani et al, 2020;Pham et al, 2012).…”

“…During pre-preparing, we put a group of ishes from a similar holding tank into a pre-treatment tank for in about 5 to 20 minutes. During this period, drugs that are to be examined could be blended with the water of the pre-treatment tank before presenting the ishes (Akhmola-Mani et al, 2020;Pham et al, 2012).…”

“…The base and the mouth of the tank had a length of 26 cm, giving a rectangular appearance to the tank. The tank was practically partitioned into several zones to help with information recording and perceptions (Akhmola-Mani et al, 2020;Pham et al, 2012). The mirror biting test was performed utilizing two methods that give a powerful way of assessing the social practices in zebra ishes (Pham et al, 2012).…”

“…At that point, we Introduced the mirror into the tank without making signi icant disturbance to the surrounding or the ish. After that, we watched the behaviour of the ish in response to the mirror for about 5 minutes (Akhmola-Mani et al, 2020;Pham et al, 2012).…”

“…This convention utilizes strong novelty of both the mirror and the tank to watch their reaction. But this convention may not be reasonable for stressed ishes (Akhmola-Mani et al, 2020;Pham et al, 2012).…”