Os conteĂșdos das glĂąndulas metatorĂĄcicas de insetos adultos do percevejo Dichelops melacanthus (Hemiptera: Pentatomidae) foram analisados. Os compostos de defesa foram identificados combinando as tĂ©cnicas de cromatografia gasosa (CG), cromatografia gasosa acoplada Ă espectrometria de massas (CG-EM) e por comparação de seus Ăndices de retenção com os de compostos padrĂ”es. Tridecano foi o componente majoritĂĄrio, seguido de quantidades menores e aproximadamente iguais de (E)-4-oxo-2-hexenal e (E)-2-octenal. TambĂ©m foram identificados (E)-2-hexenal, decano, acetato de (E)-2-hexenila, undecano, (E)-4-oxo-2-octenal, dodecano, acetato de (E)-2-octenila, 1-trideceno, tetradecano e pentadecano.The contents of metathoracic glands of adults of the stink bug Dichelops melacanthus (Hemiptera: Pentatomidae) were analyzed. Compounds were identified by gas chromatography (GC), coupled GC-mass spectrometry and matching retention indices and mass spectra with those of authentic samples. Tridecane was the major component followed by lesser and approximately equal amounts of (E)-4-oxo-2-hexenal and (E)-2-octenal. Other compounds identified include (E)-2-hexenal, decane, (E)-2-hexenyl acetate, undecane, (E)-4-oxo-2-octenal, dodecane, (E)-2-octenyl acetate, 1-tridecene, tetradecane and pentadecane.Keywords: Dichelops melacanthus, defensive compounds, short-chain unsaturated aldehydes, unsaturated acetates, tridecane
IntroductionInsects have evolved a multitude of chemical and behavioral defenses against other offensive organisms. Stink bugs, as their name suggests, produce large quantities of strong-smelling and irritating defensive chemicals, which are released when the bugs are disturbed or molested.
1In general, heteropterans produce allomones in dorsal abdominal scent glands as immatures, and in metathoracic scent glands as adults. [1][2][3] Numerous reports attest to the efficacy of the secretions of the metathoracic and abdominal scent glands as effective defenses against predation. 1,2,4 They also may have a role as alarm pheromones, 5 as has been demonstrated for similar types of compounds produced by bug species in other families. 6,7 Stink bug defensive compounds have received considerable study, and it has been shown that the types of compounds constituting the defensive chemical blends typically consist of alkane hydrocarbons and saturated and unsaturated aldehydes and esters. In adults, the defensive compounds are produced in large, well-defined, and usually colored metathoracic glands, which are not present in the immature stages.Sex pheromones have been identified from only a few phytophagous stink bug species to date. 8,9 In all cases reported, components from metathoracic glands have not been part of the pheromone blend. However, because stink bugs readily release defensive compounds when disturbed, it is difficult if not impossible to collect extracts of sex pheromones uncontaminated by these components.The main objective of this study was to characterize the defensive compounds produced in the metathoracic glands o...