Alanine to serine substitutions drive thermal adaptation in a psychrophilic diatom cytochrome c6

Metal coordination governs the antimicrobial efficacy of calcitermin derivatives

“…Ala-to-Ser substitutions are introduced with the aim of verifying their impact on metal coordination, and whether this can affect the antimicrobial activity of calcitermin. [19][20][21][22][23][24] Metal coordination and antimicrobial properties have also been investigated for the calcitermin variant truncated between positions 7 and 8 and protected at the amino and carboxyl termini (Ac-AHYHTHKE-NH 2 , WT (8)(9)(10)(11)(12)(13)(14)(15)). Compared to wild-type calcitermin, in WT (8)(9)(10)(11)(12)(13)(14)(15) the more lipophilic portion of the molecule, not directly involved in metal coordination, was excluded in order to clarify whether the coordination site -HYHTH-is sufficient to form Cu 2+ and Zn 2+ complexes with microbicidal activity.…”