ALA-induced photodynamic effect on vitality, apoptosis, and secretion of vascular endothelial growth factor (VEGF) by colon cancer cells in normoxic environment in vitro

Kawczyk‐Krupka, Aleksandra; Sieroń–Stołtny, Karolina; Latos, Wojciech; Czuba, Zenon; Kwiatek, B.; Potempa, Magdalena; Wasilewska, Katarzyna; Król, Wojciech; Stanek, Agata

doi:10.1016/j.pdpdt.2015.09.003

Cited by 18 publications

(14 citation statements)

References 37 publications

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“…Interestingly, ALA treatment also inhibited tumor growth to some extent, which might be related to its potential photodynamic efficacy on tumors. 52 − 54 As a control, the tolerance of the combined treatment of artesunate and ALA was confirmed by the comparable body weight reduction among different groups ( Figure S5c ). Consistently, the ART activation level in the mouse tumor tissue was shown to be elevated by the combination treatment, as revealed by in-gel fluorescence scan ( Figure 6 c).…”

Section: Resultsmentioning

confidence: 62%

“… 52 It has been reported to have minimal side effects and is clinically safe for cancer treatment. 52 − 54 Therefore, we introduced ALA as a potential sensitizer to enhance the efficacy of artesunate by increasing heme levels to enhance artesunate activation. We examined cancer cell viability after treatments with artesunate, artesunate + ALA, and artesunate + ALA + SA (succinylacetone, an inhibitor of heme synthesis) respectively.…”

Section: Resultsmentioning

confidence: 99%

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Mechanistic Investigation of the Specific Anticancer Property of Artemisinin and Its Combination with Aminolevulinic Acid for Enhanced Anticolorectal Cancer Activity

et al. 2017

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The antimalarial artemisinin (ART) possesses anticancer activity, but its underlying mechanism remains largely unclear. Using a chemical proteomics approach with artemisinin-based activity probes, we identified over 300 specific ART targets. This reveals an anticancer mechanism whereby ART promiscuously targets multiple critical biological pathways and leads to cancer cell death. The specific cytotoxicity of ART against colorectal cancer (CRC) cells rather than normal colon epithelial cells is due to the elevated capacity of heme synthesis in the cancer cells. Guided by this mechanism, the specific cytotoxicity of ART toward CRC cells can be dramatically enhanced with the addition of aminolevulinic acid (ALA), a clinically used heme synthesis precursor, to increase heme levels. Importantly, this novel ART/ALA combination therapy proves to be more effective than an ART monotherapy in a mouse xenograft CRC model. Thus, ART can be repurposed and potentiated by exploitation of its mechanism of action and the metabolic features of the CRC cells.

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Section: Resultsmentioning

confidence: 62%

Section: Resultsmentioning

confidence: 99%

Mechanistic Investigation of the Specific Anticancer Property of Artemisinin and Its Combination with Aminolevulinic Acid for Enhanced Anticolorectal Cancer Activity

et al. 2017

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“…On the other hand, it is important to mention that the investigation in the field of PDT in relation to a tumor angiogenic mechanism was performed primarily with animal experimental models (see Table 1 and Table 2 ). However, the utilization of less relevant 2D cell models also occurred [ 28 , 60 , 61 , 62 , 63 , 64 , 65 ] (see Table 3 ).…”

Section: Experimental Models and Their Potential Limitations Andmentioning

confidence: 99%

Potentiality, Limitations, and Consequences of Different Experimental Models to Improve Photodynamic Therapy for Cancer Treatment in Relation to Antiangiogenic Mechanism

Majerník

Jendželovský

Fedoročko

2020

Cancers

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The relevance of experimentally gained information represents a long-term debating issue in the field of molecular biology research. The loss of original conditions in the in vitro environment affects various biological mechanisms and cellular interactions. Consequently, some biochemical mechanisms are lost or critically altered. Analyses in these modified conditions could, therefore, distort the relevancy of experimentally gained information. In some cases, the similarities with original conditions are so small that utilization of simpler in vitro models seems impossible, or could occur in a very limited way. To conclude, the study of more complex phenomena places higher demands on the complexity of the experimental model. The latest information highlights the fact that the tumor angiogenesis mechanism has very complex features. This complexity can be associated with a wide range of angiogenic factors expressed by a variety of malignant and non-malignant cells. Our article summarizes the results from various experimental models that were utilized to analyze a photodynamic therapy effect on tumor angiogenic mechanisms. Additionally, based on the latest information, we present the most important attributes and limitations of utilized experimental models. We also evaluate the essential problems associated with angiogenic mechanism induction after photodynamic therapy application.

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“…The direct cytotoxic effect of PDT results from the generation of reactive forms of oxygen, which, as a result, leads to cell death through necrosis or apoptosis. Moreover, PDT applied in smaller doses of energy shows immunomodulating activity through stimulation of the immune system and influence on auto-and paracrine activity of cancer cells [45,46]. The effectiveness of PDT has been proven in the treatment of many types of cancer such as head and neck, urinary bladder, skin and colon cancer [44].…”

Section: Terapia Spersonalizowana Raka Jelita Grubegomentioning

confidence: 99%

“…Bezpośredni efekt cytotoksyczny PDT wynika z generacji reaktywnych form tlenu, co w efekcie prowadzi do śmierci komórki poprzez nekrozę lub apoptozę. Ponadto PDT stosowana w mniejszych dawkach energii wykazuje działanie immunomodulujące przez stymulację układu odpornościowego oraz wpływ na auto-i parakrynną aktywność komórek nowotworowych [45,46]. Skuteczność PDT udowodniono w leczeniu wielu nowotworów, m.in.…”

Section: Terapia Spersonalizowana Raka Jelita Grubegounclassified

Colon cancer – new strategies of treatment

Międzybrodzka

Gmyrek²,

Cieślar³

et al. 2019

Ann. Acad. Med. Siles.

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Colon cancer is one of the most frequent causes of death in the world. Despite the easily accessible diagnostic tools, the disease is still diagnosed at its advanced stage, when radical treatment is no longer possible and only palliative therapy can be provided. Cancer recurrence, which worsens the patient's prognosis, is a separate problem. Currently, scientists are seeking modern, multidirectional treatment methods, which would enable the inhibition of neoplasia, elongation of patients' lives and improvement in their quality. Immunotherapy and photodynamic therapy seem to be promising as they provide good results, also at advanced stages of cancer. Due to its cytotoxic and immunomodulating effect, photodynamic therapy exhibits an antineoplastic effect. Using monoclonal antibodies in the targeted therapy of colon cancer is a new, promising concept, which requires further studies.

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ALA-induced photodynamic effect on vitality, apoptosis, and secretion of vascular endothelial growth factor (VEGF) by colon cancer cells in normoxic environment in vitro

Cited by 18 publications

References 37 publications

Mechanistic Investigation of the Specific Anticancer Property of Artemisinin and Its Combination with Aminolevulinic Acid for Enhanced Anticolorectal Cancer Activity

Mechanistic Investigation of the Specific Anticancer Property of Artemisinin and Its Combination with Aminolevulinic Acid for Enhanced Anticolorectal Cancer Activity

Potentiality, Limitations, and Consequences of Different Experimental Models to Improve Photodynamic Therapy for Cancer Treatment in Relation to Antiangiogenic Mechanism

Colon cancer – new strategies of treatment

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