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Grewal, Burrinder S.; Naik, Aarti; Irwin, W. J.; Gooris, Gert S.; Grauw, C.J. de; Gerritsen, Hans; Bouwstra, Joke A.

doi:10.1023/a:1007595822786

Cited by 32 publications

(3 citation statements)

References 10 publications

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“…Furthermore, FLIM can be used to study percutaneous penetration. A major challenge in assessing the efficacy of topical products is to derive real-world local efficacy and toxicity data. , A number of papers have used confocal and multiphoton native fluorescence to describe the penetration of chemicals and NPs. − Therefore, in some experiments, a combination of multispectral imaging and FLIM is preferred in studying the skin penetration of xenobiotics and NPs.…”

Section: Other Fluorescence-based Applicationsmentioning

confidence: 99%

Chemistry, Biology, and Medicine of Fluorescent Nanomaterials and Related Systems: New Insights into Biosensing, Bioimaging, Genomics, Diagnostics, and Therapy

2014

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Section: Other Fluorescence-based Applicationsmentioning

confidence: 99%

Chemistry, Biology, and Medicine of Fluorescent Nanomaterials and Related Systems: New Insights into Biosensing, Bioimaging, Genomics, Diagnostics, and Therapy

2014

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“…This allows their visualization and quantification in the skin, as well as an estimation of the effect of oleic acid as a penetration enhancer [ 137 ], and the visualization of the intracellular penetration pathway [ 138 ]. Skin penetration was determined for topically applied highly fluorescent 6-carboxyfluorescein in a core droplet of the tailorable nano-emulsions [ 114 ], fluorescently labelled dextran in combination with topical enhancers [ 139 ], gold nanoparticle dispersion [ 140 ], zinc oxide nanoparticles [ 141 ], nanobeads [ 142 ], FITC dextran, Texas Red [ 120 ], FITC-labeled PLGA nanoparticles [ 136 ], and sunscreen labeled with fluorescein dye [ 143 ]. A representative image of the latter is shown in Figure 6 A.…”

Section: Multi-photon Tomography (Mpt)mentioning

confidence: 99%

Optical Methods for Non-Invasive Determination of Skin Penetration: Current Trends, Advances, Possibilities, Prospects, and Translation into In Vivo Human Studies

Darvin

2023

Pharmaceutics

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Information on the penetration depth, pathways, metabolization, storage of vehicles, active pharmaceutical ingredients (APIs), and functional cosmetic ingredients (FCIs) of topically applied formulations or contaminants (substances) in skin is of great importance for understanding their interaction with skin targets, treatment efficacy, and risk assessment—a challenging task in dermatology, cosmetology, and pharmacy. Non-invasive methods for the qualitative and quantitative visualization of substances in skin in vivo are favored and limited to optical imaging and spectroscopic methods such as fluorescence/reflectance confocal laser scanning microscopy (CLSM); two-photon tomography (2PT) combined with autofluorescence (2PT-AF), fluorescence lifetime imaging (2PT-FLIM), second-harmonic generation (SHG), coherent anti-Stokes Raman scattering (CARS), and reflectance confocal microscopy (2PT-RCM); three-photon tomography (3PT); confocal Raman micro-spectroscopy (CRM); surface-enhanced Raman scattering (SERS) micro-spectroscopy; stimulated Raman scattering (SRS) microscopy; and optical coherence tomography (OCT). This review summarizes the state of the art in the use of the CLSM, 2PT, 3PT, CRM, SERS, SRS, and OCT optical methods to study skin penetration in vivo non-invasively (302 references). The advantages, limitations, possibilities, and prospects of the reviewed optical methods are comprehensively discussed. The ex vivo studies discussed are potentially translatable into in vivo measurements. The requirements for the optical properties of substances to determine their penetration into skin by certain methods are highlighted.

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“…184 Yu and co-workers further showed that, due to skin heterogeneity, high throughput large area multiphoton imaging is critical to minimize errors in determining penetrant transport properties. 185 Finally, they have also quantitatively evaluated the effect of ultrasound in skin transport enhancement. 186 …”

Section: Applications Of Multiphoton Microscopy In Different Areasmentioning

confidence: 99%

Application of multiphoton microscopy in dermatological studies: A mini-review

Yew

Rowlands

2014

J. Innov. Opt. Health Sci.

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This review summarizes the historical and more recent developments of multiphoton microscopy, as applied to dermatology. Multiphoton microscopy offers several advantages over competing microscopy techniques: there is an inherent axial sectioning, penetration depths that compete well with confocal microscopy on account of the use of near-infrared light, and many two-photon contrast mechanisms, such as second-harmonic generation, have no analogue in one-photon microscopy. While the penetration depths of photons into tissue are typically limited on the order of hundreds of microns, this is of less concern in dermatology, as the skin is thin and readily accessible. As a result, multiphoton microscopy in dermatology has generated a great deal of interest, much of which is summarized here. The review covers the interaction of light and tissue, as well as the various considerations that must be made when designing an instrument. The state of multiphoton microscopy in imaging skin cancer and various other diseases is also discussed, along with the investigation of aging and regeneration phenomena, and finally, the use of multiphoton microscopy to analyze the transdermal transport of drugs, cosmetics and other agents is summarized. The review concludes with a look at potential future research directions, especially those that are necessary to push these techniques into widespread clinical acceptance.

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Abstract: Use of a positively charged enhancer may improve J(ss) of anionic macromolecular penetrants during passive and iontophoretic delivery. On-line visualisation of iontophoresis across SC was possible and can provide mechanistic insight into SC transport pathways.

Cited by 32 publications

References 10 publications

Chemistry, Biology, and Medicine of Fluorescent Nanomaterials and Related Systems: New Insights into Biosensing, Bioimaging, Genomics, Diagnostics, and Therapy

Chemistry, Biology, and Medicine of Fluorescent Nanomaterials and Related Systems: New Insights into Biosensing, Bioimaging, Genomics, Diagnostics, and Therapy

Optical Methods for Non-Invasive Determination of Skin Penetration: Current Trends, Advances, Possibilities, Prospects, and Translation into In Vivo Human Studies

Application of multiphoton microscopy in dermatological studies: A mini-review

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