Akurasi Alat Deteksi Sodium Dalam Urin Secara Otomatis Dengan Metode Electroda Seleksi Ion Kering

Abstract

Inductively Couple Plasma (ICP) is a standard method to detect urinary sodium. However, the urinary sodium analysis method has a limitation in which the process to free sodium from molecules is not digest thoroughly. Ionic Selected Electrode (ISE) method is the better option. A New authomatic instrument Na-K-Cl automatic digital analyser that is "Spotchem EL 1520" using ISE method (dry ISE) was developed but the application to detect urinary sodium has not yet done. The purpose of this trial was to test the accuracy of the instrument in detecting urinary sodium. A number of 100 people aged 20-64 years participated in the trial by collecting around 50 mL fresh urine. Urinary sodium was analysed by ICP method in chemical laboratory and by ISE method using the digital analyser instrument. A number of 6 out of 100 urine sample did not meet a minimum volume requirement to be analysed by ICP method but it was able to be analysed using ISE method. The instrument was able to detect urinary sodium more than 132 (SD:77,81) mmol/L compared to Laboratory ICP method 79.38 (SD: 47,50) mmol/L. The Sensitivity of the instrument to detect sodium in urine was 97.6% (95% CI : 87.1-99.9%) and the Specivicity was 58 % (95% CI: 44.7-71.9). The trial also analysed urinary creatinine with aution instrument. A number of 10 urine sample was analysed by private clinical laboratory as quality control. Aution is semi quantitave digital analyser and the result was inline with quantitative (controlled sample). The automatic digital analyser can be used as alternative for conventional analyses of urinary sodium.