Aktuelle Erkenntnisse zur Eigenfett Transplantation anhand der neuen Leitlinie „Autologe Fetttransplantation“

Prantl, Lukas; Rennekampff, Hans‐Oliver; Giunta, Riccardo E.; Harder, Yves; Heimburg, Dennis von; Heine, Norbert; Herold, Christian; Kneser, Ulrich; Lampert, F.; Machens, Hans‐Günther; Mirastschijski, Ursula; Müller, Daniela; Pallua, Norbert; Schantz, T; Schönborn, A; Ueberreiter, Klaus; Witzel, Christian; Bull, G; Rezek, D; Sattler, Gerhard; Vogt, Peter M.; Horch, Raymund E.

doi:10.1055/s-0042-117635

Cited by 23 publications

(8 citation statements)

References 2 publications

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“…Liposuction was performed as described in the S2K guideline. Briefly, a 0.9% ( w / v ) solution of sodium chloride containing adrenaline (1:200,000) was infiltrated using a 2.5-mm injection cannula (Human Med AG, Schwerin, Germany) [ 37 ]. Liposuction was performed water jet–assisted with an even negative pressure of less than 0.5 mbar (Body-Jet, Human Med AG) using 3.8-mm cannulas (Human Med AG).…”

Section: Methodsmentioning

confidence: 99%

Adipose Tissue-Derived Stem Cell Yield Depends on Isolation Protocol and Cell Counting Method

Prantl

Eigenberger

Brix

et al. 2021

Cells

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In plastic surgery, lipofilling is a frequent procedure. Unsatisfactory vascularization and impaired cell vitality can lead to unpredictable take rates in the fat graft. The proliferation and neovascularization inducing properties of adipose tissue-derived stem cells may contribute to solve this problem. Therefore, the enrichment of fat grafts with stem cells is studied intensively. However, it is difficult to compare these studies because many factors—often not precisely described—are influencing the results. Our study summarizes some factors which influence the cell yield like harvesting, isolation procedure and quantification. Stem cells were isolated after liposuction. Quantification was done using a cell chamber, colony counting, or flow cytometry with changes to one parameter, only, for each comparison. Quantification of cells isolated after liposuction at the same harvesting site from the same patient can vary greatly depending on the details of the isolation protocol and the method of quantification. Cell yield can be influenced strongly by many factors. Therefore, a comparison of different studies should be handled with care.

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Section: Methodsmentioning

confidence: 99%

Adipose Tissue-Derived Stem Cell Yield Depends on Isolation Protocol and Cell Counting Method

Prantl

Eigenberger

Brix

et al. 2021

Cells

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“…Die Liposuktion, als neueres Verfahren, bietet einerseits die Möglichkeit zur gezielten Fettdepotreduktion und dient andererseits der Fettgewinnung zur autologen Fettgewebetransplantation per Injektion. Aufgrund der insuffizienten Gefäßneubildung, der geringen Ischämietoleranz und der hohen Stoffwechselaktivität von Adipozyten zeigten Transplantationen kleiner Mengen körpereigenen Fettgewebes bislang unbefriedigende Ergebnisse mit hohen Resorptionsraten und großer Fibrosierungstendenz [5,6,7]. Auf diese Weise ließen sich bisher keine klinisch relevanten Gewebevolumina zur Deckung größerer Weichteildefekte erreichen [6].…”

Section: Operative Therapie Von Weichteildefektenunclassified

“…Während braunes Fettgewebe in erster Linie der Thermoregulation zu dienen scheint, wurde für weißes Fettgewebe lange Zeit -neben seiner Isolator-bzw. mechanischen Dämpfungseigenschaft -vornehmlich eine reine Energiespeicherfunktion angenommen [7]. Inzwischen mehren sich Hinweise darauf, dass es zwei unterschiedliche Arten von braunem Fettgewebe gibt.…”

Section: Physiologie Der Adipozytenunclassified

Tissue Engineering von Fettgewebe mittels bioabbaubaren Biomaterialien zur Weichteildefektdeckung

Klein

Aung

Haas

et al. 2018

Handchir Mikrochir plast Chir

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Soft tissue defects resulting from injuries, tumor resection, congenital anomalies or chronic wounds pose a great challenge to reconstructive surgery. The current gold standard in therapy of such defects is the tissue transplantation in terms of free or local flaps. Unfortunately, donor site morbidity remains a considerable risk of flap surgery. Therefore, tissue engineering of autologous vascularized long term stable adipose tissue constructs could enrich the therapeutic possibilities of soft tissue defects. De novo adipose tissue growing requires fundamental knowledge about this kind of tissue and its synthesis, closely linked to angiogenesis. Bioresorbable biomaterials (scaffolds) are of crucial importance for adipose tissue engineering. Simulation or replacement of extracellular matrix for tissue growth by scaffold application requires a profound understanding of cell-matrix interactions. A proper biomaterial should be capable of supporting cell adherence, proliferation and differentiation. Important features are biocompatibility and resorption without toxic metabolites. In this review, various scaffold materials are discussed and novel achievements are presented. Persisting problems of de novo adipose tissue formation are high resorption rates and small tissue volumes of adipose constructs. Adipose tissue formation in a tissue engineering chamber is an additional possibility for in vivo tissue engineering. Recent studies proof that long term stable de novo adipose tissue formation of clinically relevant tissue volumes is possible. This method, in our opinion, has the potential to improve therapeutic strategies of soft tissue defects significantly.

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“…Das Transplantat un terliegt somit nicht den Bestimmungen des Arzneimittelgesetzes ( § 4 Abs. 9 AMG), da kein industrielles Herstellungsverfahren er folgt, die etablierten Herstellungsschritte eingehalten werden und keine substantielle Verarbeitung des Gewebes stattfindet [25].…”

Section: Diskussionunclassified

Pilotstudie über die Wirkung und den Nutzen von autologen Fettgewebstransplantaten bei Rhizarthrose verglichen mit einer Kortisoninjektion – 3 Monatsergebnisse

Haas¹,

Volkmer²,

Giunta³

2017

Handchir Mikrochir plast Chir

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Preliminary data suggest that an intraarticular autologous fat transplant may be useful in osteoarthritis (OA) of the CMC-1 joint. This prospective, non-randomised pilot study investigates this new method with a view to clinical outcomes compared with intraarticular corticosteroid injection. 17 women and 7 men with an average age of 63.3 (47-75) years with symptomatic OA were included in the study. In 12 patients, a radiologically guided injection of 1-1.5 ml of adipose tissue was carried out after transumbilical liposuction of 10 ml of abdominal fat. Prior to that, the fat was homogenised using two Luer-Lock Syringes, but not centrifuged. Postoperatively, the thumb was immobilised for one week. The patients from the cortisone control group received an intraarticular injection of 10 mg (1 ml) of Triamcinolon® under sterile conditions without subsequent immobilisation. Preoperatively as well as 2, 6 and 12 weeks postoperatively, the QuickDASH and the Michigan-Hand-Questionnaire (MHQ) were analysed, the pain was measured by means of the visual analogue scale (VAS), and strength and grip force were measured. Both the cortisone group and the autologous fat group showed a significant reduction in stress pain after two weeks. Both methods had no significant effect on force. The evaluation of quality of life by means of QuickDASH and MHQ after cortisone treatment initially demonstrated an improvement in quality of life, which, however, fell below the previous level after 6 weeks. After autologous fat transplantation, quality of life increased continuously. In contrast to cortisone injection treatment, a persistent pain reduction was achieved in the fat injection group in the 3-month follow-up period, whereas cortisone injection only resulted in a short-term reduction of pain lasting about 6 weeks. Long-term follow-up is required now to show how long the effect of autologous fat transplantation in OA can be sustained.

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Aktuelle Erkenntnisse zur Eigenfett Transplantation anhand der neuen Leitlinie „Autologe Fetttransplantation“

Cited by 23 publications

References 2 publications

Adipose Tissue-Derived Stem Cell Yield Depends on Isolation Protocol and Cell Counting Method

Adipose Tissue-Derived Stem Cell Yield Depends on Isolation Protocol and Cell Counting Method

Tissue Engineering von Fettgewebe mittels bioabbaubaren Biomaterialien zur Weichteildefektdeckung

Pilotstudie über die Wirkung und den Nutzen von autologen Fettgewebstransplantaten bei Rhizarthrose verglichen mit einer Kortisoninjektion – 3 Monatsergebnisse

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