AKTIVITAS SENYAWA BIOAKTIF SELADA LAUT (Ulva lactuca) SEBAGAI ANTIOKSIDAN PADA MINYAK IKAN The Activity of Bioactive Compounds from Sea Lettuce (Ulva lactuca) as Antioxidant in Fish Oil

Abstract

ABSTRAK Minyak ikan adalah produk perikanan yang mempunyai asam lemak tak jenuh tinggi yang rentan akan oksidasi. Penghambatan oksidasi dilakukan dengan penambahan antioksidan pada minyak. Penggunaan bahan alami sebagai antioksidan dari U. lactuca mampu menghambat oksidasi tersebut. Tujuan penelitian ini adalah untuk mengetahui efektifitas antioksidan selada laut (U. lactuca) dalam menghambat oksidasi. Materi yang digunakan dalam penelitian ini adalah ekstrak U. lactuca, dan minyak ikan. Metode penelitian yang digunakan adalah experimental laboratories dengan menggunakan Rancangan Acak Lengkap (RAL) faktorial yaitu faktor konsentrasi ekstrak U. lactuca (0%; 0,1%; 0,2% dan 0,3%) dan faktor lama penyimpanan (hari ke-0, hari ke-5 dan hari ke-10). Data peroxide value (PV) dan thiobarbituric acid (TBA) dianalisis menggunakan uji ANOVA, dilakukan uji lanjut Beda Nyata Jujur (BNJ), jika ada interaksi perlakuan. Hasil penelitian pendahuluan didapatkan rendemen ekstrak U. lactuca dengan pelarut etanol 96% sebesar 13,549%, nilai fenol 4,59%, flavonoid 0,59% dan aktivitas antioksidan IC50 sebesar 60,975 ppm (kuat). Hasil uji asam lemak pada minyak ikan dengan GC-MS menunjukkan kandungan asam lemak yang paling banyak adalah asam lemak tak jenuh (asam linoleat (omega -6) dan (omega-3)). Hasil penelitian utama didapatkan nilai PV berkisar antara 20,141 sampai 38,196 mEq/kg, dan nilai TBA berkisar antara 10,794 sampai 32,592 mg malonaldehid/kg pada hari ke-0. Berdasarkan hasil penelitian menunjukkan bahwa adanya interaksi antara konsentrasi ekstrak U. lactuca dan lama penyimpanan berpengaruh nyata (P<0,05). Ekstrak U. lactuca 0,2% menghasilkan angka peroksida terendah yaitu 25,606±0,116 mEq/kg, dan 0,1% menghasilkan angka TBA terendah yaitu 26,802 ±0,309 malonaldehide/kg pada hari ke-10. Kata kunci: Ekstrak U. lactuca, Oksidasi, Minyak Ikan, Antioksidan ABSTRACT Fish oil is a fishery product that contains high amount of unsaturated fatty acids, which was easily oxidated. This oxidation process can be inhibited by adding antioxidants. The use of natural antioxidants from U. lactuca known can inhibits the oxidation. The aim of this research was examinated the effectiveness of antioxidants from sea lettuce (U. lactuca) in inhibiting oxidation. Materials used in this research were U. lactuca extract, and fish oil. The methods used are experimental laboratories, and analyzed using Completely Randomized Design (CRD) factorial pattern is divided by a factor of U. lactuca extract concentrations (0%, 0.1%, 0.2% and 0.3%) and factor storage time (days 0, day 5 and day 10). The peroxide value (PV) and thiobarbituric acid (TBA) were analyzed using ANOVA test, then further carried test Honestly Significant Difference (HSD), a treatment interaction. Preliminary results obtained were the yield of U. lactuca extract with 96% ethanol was 13.549%, the phenol content was 4.59%, flavonoids was 0.59%, and the antioxidant activity of IC50 was 60.975 ppm (strong). The results analyzing of fatty acids in fish oil by GC-MS showed that the fatty acid content is at most unsaturated fatty acids. The main research results were PV values obtained were range from 20.141 to 38.196 mEq/kg, and TBA values were ranged from 10.794 to 32.592 mg/kg. Based on the research results show that the interaction between U. lactuca extract concentration and storage time significantly (P<0,05) . The result of 0,2% U. lactuca exctract contain peroxide value 25,606±0,116 mEq/kg, and 0,1 % exctract containt TBA value 26,802±0,309 malonaldehide/kg in the tenth day. Keywords: U. lactuca, Oxidation, Fish Oil, Antioxidant

Comparative Study on Antioxidant Activities, Total Phenolic Compound and Pigment Contents of Tropical Spirulina platensis, Gracilaria arcuata and Ulva lactuca Extracted in Different Solvents Polarity

“…Every species has a different compounds which affect its antioxidant activity and depends on extraction method, season, location and the species used in the study [42]. In this research, antioxidant activity of GAE was better than of G. verrucosa from Pok Tunggal water (IC50= 188.53 ppm) [13], while antioxidant activity of ULE was still relatively low compared by U. lactuca extract [43] that have strong antioxidant activity (IC50= 60.975 ppm). The different result occurs because the antioxidant activity are varied, depends on the species of seaweed [44], location [45] and seasons [14], respectively.…”

Antibacterial activity of crude extracts Ulva lactuca against Staphyloccocus aureus and Propionibacterium acne

“…As a result, polar or non-polar compounds are also extracted [26]. In addition, ethanol solvent can attract flavonoid compounds optimally [27].…”

Pendugaan Umur Simpan Ikan Patin Salai Menggunakan Metode Akselerasi Dengan Kemasan HDPE dan Teknik Pengemasan Aluminium Foil

“…Satu molekul MDA akan berikatan dengan dua molekul TBA yang kemudian membentuk kompleks MDA-TBA yang menghasilkan warna merah (Hunaefi dan Ulfat, 2019). Menurut Arbi et al, (2017) nilai TBA merupakan indeks yang digunakan untuk menentukan derajat oksidasi lemak dan dihitung berdasarkan jumlah malonaldehid (MDA) yang terbentuk.…”