Aktivitas In Vitro Penurun Gula Darah dari Ekstrak Buah Parijoto (Medinilla speciosa B.)

Abstract

Diabetes mellitus is one of the highest causes of death in the world, with symptoms of increased blood glucose levels (hyperglycemia). One of the efforts made as a treatment and prevention of DM is through complementary therapy using natural ingredients. Parijoto fruit (<em>Medinilla speciosa</em>) contains flavonoid compounds potential to reduce glucose levels. The study aimed to analyze the effectiveness of ethanol extracts and its fractions of parijoto fruit (EEBP) to decrease glucose levels in vitro. The study was conducted at the Ngudi Waluyo University Laboratory in April to August 2018. Identification and screening of flavonoid compounds from ethanol extracts and its fractions of parijoto fruit were performed qualitatively and by thin-layer chromatography (TLC). <em>In vitro</em> testing for antidiabetic activity of EEBP was performed with non-enzymatic reaction by Nelson Somogyi method (ethanol extract and its fractions). Experiment was arranged in a randomized block design, with 12 treatments and three replications. The concentrations of parijoto fruit tested were 10 ppm, 20 ppm, 30 ppm, 40 ppm, 50 ppm, and 60 ppm. The results of qualitative identification and TLC showed that the ethanol extract and its fraction of EEBP contained flavonoid compounds. At a concentration of 30 ppm, EEBP was able to reduce glucose levels by 42.43 %, while the ethanol fraction decreased glucose levels by 83.38 %. The results showed parijoto fruit ethanol extract and its fractions were sources of antidiabetic. Hence its pharmacologically effect was necessary to be further studied.

Alginate-Based Nanoencapsulation on Ultrasonic-Assisted Extraction of Parijoto Fruit (Medinilla Speciosa Blume) and Its Antioxidant Activity

“…Another study by Muhammad et al, (2021) also stated that ethanol solvents produce a greater yield than other organic solvents. The results of the phytochemical screening in Table 2 show the presence of secondary metabolites of flavonoids, tannins, and saponins in Parijoto fruit extract, it is in line with research conducted by Vifta and Advistasari (2018). The resulting Parijoto fruit extract contains a water content by the quality of the extract, which is equal to 2.86%, and the resulting extract is free from ethanol solvent.…”

“…It is also known that the Parijoto fruit extracts and fractions have extremely strong antioxidant activities (Vifta & Luhurningtyas, 2019). According to research by Vifta and Advistasari (2018) the Parijoto fruit's ethanol and ethyl acetate fractions both contain secondary metabolites of flavonoids, tannins, and saponins. The majority of pharmaceutical substances produced from natural ingredients have flaws, such as limited water solubility, a lack of permeability to cross the absorption barrier, and large particle sizes that reduce their bioavailability.…”

Antibacterial Activity of Scurrula ferruginea (Roxb. Ex Jack) Leaves Extract Against Staphylococcus aureus and Escherichia coli

“…Therefore, careful selection is required, as the proper solvent can attract active compounds perfectly. The principle of solubility of substances is a similar polarity, namely 'like dissolve like', where the compounds with similar polarity dissolve each other (Suhendra et al, 2019;Vifta & Advistasari, 2018). As shown in Table 2, total flavonoid, phenolic, and tannin content of Scurrula ferruginea leaf ethanolic extract are 7.41 ± 0.38 mg QE/g extract, 1672 ± 50.99 mg GAE/g extract, and 1.43 ± 0.15 mg TAE/g extract, respectively.…”

Penetapan Kadar Fenolik dan Flavonoid Total Ekstrak Etanol Buah Parijoto (Medinilla speciosa Blume) dengan Metode Spektrofotometri UV-Vis

“…Ekstrak etanol dari buah parijoto diketahui mengandung flavonoid yang berperan dalam penurunan kadar gula dalam darah (Vifta & Advistasari, 2018). Ekstrak etanol buah parijoto juga diketahui memiliki aktivitas sebagai antioksidan (Pujiastuti & Saputri, 2019;Vifta et al, 2019). Selain itu, ekstrak etanol buah parijoto juga memiliki aktivitas sebagai antikanker pada sel kanker payudara (T47D) (Tusanti et al, 2014).…”