Aktivitas Antibiofilm Ekstrak dan Fraksi-Fraksi Biji Pinang (Areca catechu L.) Terhadap Staphylococcus aureus ATCC 25923

Tobi, Claudius Hendraman B.; Saptarini, Opstaria; Rahmawati, Ismi

doi:10.20961/jpscr.v7i1.43698

Cited by 3 publications

(2 citation statements)

References 7 publications

(9 reference statements)

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“…Kuersetin merupakan senyawa flavonoid yang banyak ditemukan pada sayuran dan buahbuahan. Selain memiliki aktivitas antioksidan yang sangat kuat [8], [9], [10], kuersetin juga memiliki aktivitas biologi lainnya seperti antibakteri [11], antibiofilm [12], antiinflamasi [13], dan antikanker [14]. Beberapa penelitian telah membuktikan bahwa kuersetin memiliki aktivitas yang signifikan dalam menghambat beberapa sel kanker seperti kanker payudara, prostat, kolon dan paru-paru [14], [15].…”

Section: Pendahuluanunclassified

Identifikasi Senyawa Flavonoid dan Uji Aktivitas Antibakteri Ekstrak Terpurifikasi Daun Beluntas (Pluchea Indica L.) terhadap Staphylococcus aureus

Tobi,

Pratiwi

2023

J. Sains Kes.

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One of the native Indonesian plants that can be used as medicine is beluntas (Pluchea indica L.). The biological activities in beluntas leaves cannot be separated from the role of secondary metabolite compounds contained in them, one of which is flavonoid compounds. The aim of this research was to determine the presence and levels of flavonoid compounds in purified extracts of beluntas leaves, as well as to determine the antibacterial activity of the extracts. The research began with extraction and purification of the extract, qualitative testing of flavonoid compounds using Thin Layer Chromatography (TLC), and determination of total flavonoid levels and testing of antibacterial activity against Staphylococcus aureus using the solid dilution method. The results of the research showed that the purified extract of beluntas leaves contained flavonoid compounds which were marked by stains on the TLC plate with an Rf value that was almost the same as the pure quercetin compound, namely 0.96 (quercetin) and 0.93 (sample of purified beluntas leaf extract). The total flavonoid level obtained in the sample was 27.9 mg/g quercetin. The purified extract of beluntas leaves has antibacterial activity with a minimum kill concentration of 5%. The conclusion that obtained from the results of the research showed that the purified extract of beluntas leaves contains flavonoid compounds and can be used and developed as a medicinal ingredient, one of which is antibacterial. Keywords: Beluntas, Flavonoid, Kualitatif, kuantitatif, Staphylococcus aureus Abstrak Salah satu tanaman asli Indonesia dapat dimanfaatkan sebagai obat yaitu beluntas (Pluchea indica L.). Aktivitas biologis pada daun beluntas tidak terlepas dari peranan senyawa metabolit sekunder yang terkandung di dalamnya, salah satunya senyawa flavonoid. Tujuan penelitian ini untuk mengetahui keberadaan dan kadar senyawa flavonoid pada ekstrak terpurifikasi daun beluntas, serta mengetahui aktivitas antibakteri ekstrak. Penelitian dimulai dari proses ektraksi dan purifikasi ekstrak, pengujian senyawa flavonoid secara kualitatif menggunakan Kromatografi Lapis Tipis (KLT), dan penentuan kadar flavonoid total serta pengujian aktivitas antibakteri terhadap Staphylococcus aureus menggunakan metode dilusi padat. Hasil penelitian menunjukkan bahwa ekstrak terpurifikasi daun beluntas memiliki senyawa flavonoid yang ditandai dengan noda kuning-kecokelatan pada plat KLT dengan nilai Rf yang hampir sama dengan senyawa kuersetin murni yaitu 0,96 (kuersetin) dan 0,93 (sampel ekstrak terpurifikasi daun beluntas). Kadar flavonoid total yang diperoleh pada sampel yaitu 27,9 mg/g kuersetin. Ekstrak terpurifikasi daun beluntas memiliki aktivitas antibakteri dengan Konsentrasi Bunuh Minimum sebesar 2,5%. Kesimpulan yang dapat diambil dari hasil penelitian menunjukkan ekstrak terpurifikasi daun beluntas mengandung senyawa flavonoid dan dapat dimanfaatkan serta dikembangkan sebagai bahan obat, salah satunya sebagai antibakteri. Kata Kunci: Beluntas, Flavonoid, Kualitatif, Kuantitatif, Staphylococcus aureus

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Section: Pendahuluanunclassified

Identifikasi Senyawa Flavonoid dan Uji Aktivitas Antibakteri Ekstrak Terpurifikasi Daun Beluntas (Pluchea Indica L.) terhadap Staphylococcus aureus

Tobi,

Pratiwi

2023

J. Sains Kes.

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“…S. aureus is a Gram-positive bacterium that causes infectious diseases of the skin [1]. These bacteria can cause various types of infections ranging from minor skin infections, food poisoning, to systemic infections [2]. Infection is one of the complications that are often found in the care of patients with DM.…”

Section: Introductionmentioning

confidence: 99%

Bioactivity Tracing of the Ethanol Extract of Bajakah Tampala (Spatholobus littoralis Hassk.) Typical Plant of Kalimantan Island as Antibiofilm of Staphylococcus aureus

Hamzah¹,

Pratiwi

Jabbar

et al. 2023

Open Access Maced J Med Sci

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BACKGROUND: Free radicals (oxidants) can cause skin irritation/damage which can be a manifestation of minor skin infections. Not only that, one of the complications of the disease that can arise is diabetes mellitus (DM) with diabetic foot ulcers (LKD). LKD is very susceptible to exposure to microorganisms and develops into diabetic foot infection (DFI). DFI is associated with the presence of biofilms in wounds especially those caused by Staphylococcus aureus infection. Bajakah tampala (Spatholobus littoralis hassk) is one of the native plants of Indonesia which has been known to have antibacterial activity, while its antibiofilm activity has not been studied. Evaluation of antibiofilms from the tampala bajakah plant can be of good novelty value, as well as support success in the treatment of infectious. AIM: This study aims to determine the effectiveness of the ethanol extract of the Bajakah tampala plant from East Kalimantan in inhibiting and eradicating the formation of S. aureus biofilms. MATERIALS AND METHODS: The planktonic and biofilm inhibition tests were carried out using the microtiter broth method. Antibiofilm activity of Bajakah tampala ethanol extract against S. aureus was analyzed by calculating the minimum biofilm inhibitor concentration (MBIC50) and the biofilm eradication activity calculating the minimum biofilm eradication concentration (MBEC50). In addition, we also carried out additional verification tests using the DPPH method by calculating the inhibitory concentration (IC50) parameter value. RESULTS: The results showed that the 1% bajakah tampala extract gave mid-phase antibiofilm activity of 80.23% w/v ± 0.01, maturation phase of 77.23% w/v ± 0.01 and eradication with a large inhibition of 75.56% w/v ± 0.01. In the DPPH test, the IC50 value was 5.9 ppm with a very strong category. CONCLUSION: Thus, it can be concluded that the ethanolic extract of the Bajakah tampala plant has a high potential to be developed as a candidate for new antibiofilm drugs against S. aureus biofilms.

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Potential of Indonesian Plants as Polymicrobial Anti-Biofilm

Sari,

Pratiwi,

Murti

et al. 2024

Borneo J Pharm

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Biofilm infection occurs in 80% of chronic infections caused by 60% of biofilms from plankton cells and polymicrobial biofilms. Due to synergistic interactions between species, infections caused by polymicrobial biofilms are more virulent than monospecies biofilm infections. New anti-biofilm candidates are constantly being developed by tracing the content of active compounds from medicinal plants native to Indonesia. The need to find new plant sources that have the potential as anti-biofilms is increasingly needed along with increasing microbial resistance. Various studies show that active compounds that have anti-biofilm potential are polyphenols, quercetin, curcumin, gallic acid, and ferulic acid. The mechanism of action of anti-biofilms is through the prevention of attachment and formation of biofilms, inhibition of quorum sensing, and inhibition of gene expression in microbes.

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Aktivitas Antibiofilm Ekstrak dan Fraksi-Fraksi Biji Pinang (Areca catechu L.) Terhadap Staphylococcus aureus ATCC 25923

Cited by 3 publications

References 7 publications

Identifikasi Senyawa Flavonoid dan Uji Aktivitas Antibakteri Ekstrak Terpurifikasi Daun Beluntas (Pluchea Indica L.) terhadap Staphylococcus aureus

Identifikasi Senyawa Flavonoid dan Uji Aktivitas Antibakteri Ekstrak Terpurifikasi Daun Beluntas (Pluchea Indica L.) terhadap Staphylococcus aureus

Bioactivity Tracing of the Ethanol Extract of Bajakah Tampala (Spatholobus littoralis Hassk.) Typical Plant of Kalimantan Island as Antibiofilm of Staphylococcus aureus

Potential of Indonesian Plants as Polymicrobial Anti-Biofilm

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