Aktivitas Antibakteri Ekstrak Metanol Kulit Buah Kawista (Limonia acidissima) Terhadap Bakteri Staphylococcus aureus

Abstract

Kawista (Limonia acidissima) adalah tumbuhan famili Rutaceae yang tidak hanya ada di India tetapi juga ada di Indonesia. Kulit buah kawista diketahui mengandung senyawa flavonoid seperti rutin, naringin, hesperidin, sinensetin yang diketahui memiliki aktivitas antibakteri. Penelitian antibakteri dari ekstrak metanol kulit buah kawista di Indonesia terhadap Staphylococcus aureus belum diuji. Penelitian ini bertujuan untuk mengetahui aktivitas antibakteri ekstrak metanol kulit buah kawista terhadap bakteri S.aureus. Metode yang digunakan meliputi pembuatan ekstrak secara maserasi dengan metanol dan pengujian aktivitas antibakteri dengan metode difusi cakram pada ekstrak dengan konsentrasi 25%, 50% dan 75%, kontrol positif klindamisin dan kontrol negatif aquadest. Hasil penelitian menunjukkan bahwa ekstrak metanol kulit buah kawista memiliki aktivitas antibakteri terhadap S. aureus pada konsentrasi 25%, 50% dan 75% dengan diameter daya hambat secara berurut yaitu 15,49 mm, 17,37 mm, dan 22,49 mm. Ekstrak metanol kulit buah kawista konsentrasi 75% memiliki efektivitas yang sama dengan kontrol positif klindamisin (20,05 mm).

Methanol Extract Inhibition’s Effectiveness of Kawista Fruit Var. Dompu on E-Coli Through in-Vitro

“…The yield presentation is a comparison of the final weight with the initial weight multiplied by 100%, the yield is also related to the amount of bioactive content contained in the simplisia (Dewatisari et al, 2018). The extraction results of 373.27 g of dried fruit flesh and Kawista seed powder using 800 mL of solvent produced a thick extract of fruit flesh and Kawista seeds as much as 23.5 grams, then calculated the percentage of extract yield and obtained as much as 6.296%, while the percentage of extract yield obtained by previous research, namely research conducted by Kusuma et al (2022), a yield of 24.8% was obtained from the powder of Kawista fruit flesh weighing 273 g. The difference in yield percentage results obtained with research conducted by Kusuma et al This is because in this study the sample used is estimated to still contain a lot of fat so that it cannot be drawn by methanol and the difference in the weight of the extracted powder. Particle size also affects the percentage of yield produced, where research by Maulida and Guntarti (2015) states that reducing the particle size of simplisia can increase the amount of active substance and the amount of extract yield.…”

“…This difference can occur due to the difference in polarity of the solvent. Methanol has a polarity of 34, while water has a polarity of 74 (I. M. Kusuma et al, 2022). The more polar the solvent of the chemical components in the plant sample will be perfectly extracted, resulting in more extracts (I. M. Kusuma et al, 2022).…”

“…Testing antibacterial activity in Kawista fruit flesh using methanol extracts of fruit flesh and Kawista seeds with various concentrations, namely 25%, 50%, 75% and 100%. The concentration was taken based on research conducted by Kusuma and Jastian (2021) and Kusuma et al (2022) the two studies used methanol extracts of Kawista fruit peels with concentrations of 25%, 50% and 75% but used different bacteria, namely Propionibacterium acnes and Staphylococcus aureus bacteria, respectively. The results of Kusuma and Jastian's (2021) research show that methanol extract of Kawista fruit peel at concentrations of 25%, 50% and 75% has anti-bacterial activity on P.acnes with inhibitory diameter values sequentially, namely 10.80 mm, 12.40 mm and 13.31 mm, in the strong category.…”

EFEKTIVITAS SIRUP EKSTRAK DAUN MURBEI (Morus alba L.) TERHADAP PENURUNAN ASAM URAT PADA MENCIT (Mus Musculus)

“…Sampel darah di ambil dari bagian ujung ekor mencit yang dipotong pada jam ke 1,5 dihitung setelah pemberian hati ayam. Persentase penurunan kadar asam urat dihitung berdasarkan rumus (Kusuma, et al 2019):…”

“…Penurunan kadar asam urat mencit setelah pemberian obat atau perlakuan dapat terlihat pada jam ke t1,5. Hal ini sesuai dengan penelitian Kusuma, et al (2019) bahwa kerja allopurinol 10 mg/kg BB efektif pada jam Jurnal Tumbuhan Obat Indonesia, 13(2): 70-77, Desember 2020 ke1,5 (t1,5). Data pengecekan kadar asam urat darah pada jam ke2 (t2) sampai dengan t5 (5 jam berikutnya) tidak dapat dianalisis karena banyak hasil pengecekan kadar asam urat darah yang tidak terdeteksi oleh alat.…”