Aktivitas Antibakteri Ekstrak Etanol Dari Lima Tanaman Terhadap Bakteri Staphylococcus Epidermidis Dengan Metode Mikrodilusi M7 – A6CLSI

Abstract

Abstrak Antibacterial Activity Of Five Plant Ethanol Extract Against Staphylococcus Epidermidis Bacteria With Microdilution M7 -A6CLSI Method AbstractThe antibacterial activity of Leucaena leucocephala, Camellia sinensis, Psidium guajava L, Anredera cordifolia (Ten.) Steenis and Solanum nigrum L. leafs ethanol extract against Staphylococcus epidermidis using microdillution method has been studied. Using microdillution method with tetracycline, chloramfenicol and amoxicillin as antibiotic reference. Leucaena leucocephala, Camellia sinensis and Psidium guajava L. leafs ethanol extract had activity to inhibit bacteria. And the other leafs ethanol extract had unsignificant activity againts bacteria. The Minimum Inhibitory Concentration (MIC) of Leucaena leucocephala leafs ethanol extract againts Staphylococcus epidermidis were 62,5 µg/ml. The Minimum Bactericidal Concentration (MBC) of Camellia sinensis and Psidium guajava L were 125 µg/ ml.

Uji Efektivitas Ekstrak Biji Lamtoro (Leucaena leucocephala) sebagai Insektisida terhadap Kecoa Amerika (Periplaneta americana)

“…Chahyono dan Suryana mengemukakan bahwa biji lamtoro mengandung semyawa aktif seperti flavonoid, tanin, saponin, alkaloid dan mimosin [7], [8] . Namun, sampai saat ini belum ada informasi mengenai pemanfaatan biji lamtoro sebagai insektisida alami.…”

UJI AKTIVITAS ANTIBAKTERI FRAKSI N-HEKSAN, ETIL ASETAT, DAN BUTANOL DAUN PETAI CINA (Leucaena leucocephala (Lam.) de Wit) TERHADAP BAKTERI Propionibacterium acnes DAN Staphylococcus epidermidis SECARA IN VITRO

“…Pada penelitian terdahulu diketahui bahwa ekstrak etanol daun petai cina terbukti mempunyai aktivitas antibakteri terhadap Staphylococcus epidermidis [5] dan Staphylococcus aureus [6]. Penelitian antibakteri pada fraksi daun petai cina belum banyak dilakukan, oleh karena itu dinilai penting untuk melakukan penelitian lebih lanjut terhadap fraksi senyawa daun petai cina yang memiliki aktivitas antibakteri paling baik.…”

Antibacterial Activity of Leucaena leucocephala Leaf Extract Ointment against Staphylococcus aureus and Staphylococcus epidermidis

“…The antibacterial activity of LLE ointment is caused by the ethanol extract of L. leucocephala leaf that contains secondary metabolites. Previous research has found that LLE comprises flavonoids, tannins, and saponins that have antibacterial properties (Suryana et al, 2017). Also, it possesses phytol (terpene compounds) and squalene (triterpene compounds), which are efficacious as antibacterial (Zayed and Samling, 2016;Suryana et al, 2017).…”

“…Another study with Gas Chromatography (GC) has identified the presence of terpenes, along with squalene as a secondary metabolite in LLE (Awe, 2013;Mohammed et al, 2015;Negi et al, 2016). Flavonoids, tannins, terpenes, and triterpene are known as phytochemical substances that have antibacterial properties (Aderibigbe, 2011;Mohammed et al, 2015;Negi et al, 2016;Zayed and Samling, 2016;Suryana, 2017). In other words, previous studies have confirmed that L. leucocephala leaf extract (LLE) has antibacterial properties.…”

“…Several studies have shown that L. leucocephala leaf extract (LLE) possesses several chemical compounds. Research using Thin Layer Chromatography (TLC) has proved that the ethanol extract of L. leucocephala leaf contains alkaloids, flavonoids, saponins, and tannins (Suryana, 2017). Another study with Gas Chromatography (GC) has identified the presence of terpenes, along with squalene as a secondary metabolite in LLE (Awe, 2013;Mohammed et al, 2015;Negi et al, 2016).…”