AKT Signaling Mediates IGF-I Survival Actions on Otic Neural Progenitors

Aburto, María Rodríguez; Magariños, Marta; León, Yolanda; Varela-Nieto, Isabel; Sánchez-Calderón, Hortensia

doi:10.1371/journal.pone.0030790

Cited by 54 publications

(64 citation statements)

References 64 publications

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“…The other genes were involved in two critical signaling pathway, namely PI3K/Akt pathway and Wnt signal pathway. The PI3K-Akt pathway participated in early infection of some exogenous avian leucosis viruses [46] and could mediate IGF-1 survival during the otic neuronal progenitor phase of early inner ear development [47]. The GPC1 gene involved in Wnt pathway was of great importance in chick, which can regulate the signaling mechanisms in early formation of the trigeminal sensory system and cell proliferation hence affected reproduction performance [48,49].…”

Section: Discussionmentioning

confidence: 99%

Genome-Wide Detection of Selective Signatures in Chicken through High Density SNPs

Liu

Sun

et al. 2016

PLoS ONE

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Chicken is recognized as an excellent model for studies of genetic mechanism of phenotypic and genomic evolution, with large effective population size and strong human-driven selection. In the present study, we performed Extended Haplotype Homozygosity (EHH) tests to identify significant core regions employing 600K SNP Chicken chip in an F2 population of 1,534 hens, which was derived from reciprocal crosses between White Leghorn and Dongxiang chicken. Results indicated that a total of 49,151 core regions with an average length of 9.79 Kb were identified, which occupied approximately 52.15% of genome across all autosomes, and 806 significant core regions attracted us mostly. Genes in candidate regions may experience positive selection and were considered to have possible influence on beneficial economic traits. A panel of genes including AASDHPPT, GDPD5, PAR3, SOX6, GPC1 and a signal pathway of AKT1 were detected with the most extreme P-values. Further enrichment analyses indicated that these genes were associated with immune function, sensory organ development and neurogenesis, and may have experienced positive selection in chicken. Moreover, some of core regions exactly overlapped with genes excavated in our previous GWAS, suggesting that these genes have undergone positive selection may affect egg production. Findings in our study could draw a comparatively integrate genome-wide map of selection signature in the chicken genome, and would be worthy for explicating the genetic mechanisms of phenotypic diversity in poultry breeding.

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Section: Discussionmentioning

confidence: 99%

Genome-Wide Detection of Selective Signatures in Chicken through High Density SNPs

Liu

Sun

et al. 2016

PLoS ONE

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“…During inner ear development, PI3K/Akt signaling has also been associated with insulin-like growth factor 1 (IGF-1). Mutation of the gene encoding its receptor (Igfr) is associated with severe cochlear defects and deafness [18], [19].…”

Section: Introductionmentioning

confidence: 99%

Conditional Deletion of Pten Leads to Defects in Nerve Innervation and Neuronal Survival in Inner Ear Development

et al. 2013

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All cellular phenomena and developmental events, including inner ear development, are modulated through harmonized signaling networks. Phosphatase and tensin homolog deleted on chromosome 10 (PTEN), a tumor suppressor, is a major signaling component involved in cross talk with key regulators of development; i.e., Wnt, Notch, and bone morphogenetic proteins. Although Pten function has been studied in various systems, its role in inner ear development is poorly understood. Here, we used inner ear-specific Pten conditional knockout mice and examined the characteristics of the inner ear. In a detailed analysis of the phenotype, reduced cochlear turning and widened epithelia were observed. Phalloidin staining of sensory epithelium revealed that hair cell patterns were disturbed; i.e., additional rows of hair cells were discovered. The neural abnormality revealed a reduction in and disorganization of nerve fibers, including apoptosis at the neural precursor stage. Pten deficiency induced increased phosphorylation of Akt at Ser473. The elevation of inhibitory glycogen synthase kinase 3β Ser9 phosphorylation (pGSK3β) was sustained until the neuronal differentiation stage at embryonic day 14.5, instead of pGSK3β downregulation. This is the first report on the influence of Pten/Akt/GSK3β signaling on the development of spiral ganglia. These results suggest that Pten is required for the maintenance of neuroblast number, neural precursors, and differentiation in the inner ear.

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“…In addition, the fraction of injured urethras whose damage extended to corpus spongiosum tissue was the lowest in group 1, suggesting IGF‐1 prevented spongiofibrosis. IGF‐1 has been shown to have beneficial effects on antifibrosis and tissue regeneration, which are plausible explanations for this result . We should investigate the mechanisms of cell proliferation and antifibrotic effects of IGF‐1.…”

Section: Discussionmentioning

confidence: 95%

Insulin‐like growth factor 1 sustained‐release collagen on urethral catheter prevents stricture after urethral injury in a rabbit model

et al. 2019

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Objectives To evaluate the preventive effect of an insulin‐like growth factor 1 sustained‐release collagen urethral catheter on urethral stricture after urethral injury in a rabbit model. Methods We made urethral catheters coated either with insulin‐like growth factor 1 impregnated collagen or with only collagen, and we divided 19 male Japanese white rabbits into three groups according to the kind of catheter inserted immediately after the rabbit's urethra was injured by electrocoagulation. Group 1 (n = 7) had a catheter coated with insulin‐like growth factor 1 impregnated collagen inserted; group 2 (n = 7) had a catheter coated with only collagen inserted; and group 3 (n = 5) had an uncoated catheter inserted. A total of 14 days later, the injured urethras were evaluated by urethrography and urethroscopy, and were also histologically examined. Results Urethrography showed that the ratio of the urethral lumen diameter in injured urethra to that in normal urethra was the largest in group 1 (P < 0.0001). In addition, five of the seven rabbits in group 1 (71.4%) had a urethral lumen large enough for passage of a urethroscope, a fraction larger than the corresponding fractions in groups 2 (57.1%) and 3 (20%). On histological analysis, the injured area not covered with regenerated urethral epithelium tended to be smaller in group 1 than the other two groups, but the mean difference was not significant (P = 0.19). Conclusions An insulin‐like growth factor 1 sustained‐release collagen urethral catheter significantly improves wound healing and prevents urethral stricture after urethral injury.

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AKT Signaling Mediates IGF-I Survival Actions on Otic Neural Progenitors

Cited by 54 publications

References 64 publications

Genome-Wide Detection of Selective Signatures in Chicken through High Density SNPs

Genome-Wide Detection of Selective Signatures in Chicken through High Density SNPs

Conditional Deletion of Pten Leads to Defects in Nerve Innervation and Neuronal Survival in Inner Ear Development

Insulin‐like growth factor 1 sustained‐release collagen on urethral catheter prevents stricture after urethral injury in a rabbit model

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