AKR2A participates in the regulation of cotton fibre development by modulating biosynthesis of very‐long‐chain fatty acids

Hu, Wenjun; Chen, Lin; Qiu, Xing‐Biao; Jia, Wei; Lu, Haojie; Sun, Guochang; Ma, Xiongfeng; Yang, Zuoren; C, Zhu; Hou, Yuqi; Han, Xiao; Sun, Chunyan; Hu, Rongbin; Cai, Yifan; Zhang, Hong; Li, Fuguang; Shen, Guoxin

doi:10.1111/pbi.13221

Cited by 23 publications

(13 citation statements)

References 51 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The identified DELs and DEGs in comparisons of 0 DPA vs -2 DPA and 5 DPA vs 0 DPA of ZM24 and fl indicated that many lncRNAs and coding genes are involved in the fiber initiation and primary development, while few lncRNAs and coding genes may involve the ovule development. The analysis of the DEGs further showed that fatty acid metabolism, very long strain fatty acid synthesis and sugar metabolism play important roles in the fiber initiation of ZM24, supporting the previous results [15,39]. Moreover, some MYB family, bHLH type TFs encoding genes were also identified the important roles in fiber initiation, which is in agreement with the function of these TFs in previous research [10,16,29,36,40,49,50].…”

Section: Discussionsupporting

confidence: 89%

RNA-Seq with a novel glabrous-ZM24fl reveals some key lncRNAs and the associated targets in fiber initiation of cotton

et al. 2022

Self Cite

View full text Add to dashboard Cite

Background Cotton fiber is an important natural resource for textile industry and an excellent model for cell biology study. Application of glabrous mutant cotton and high-throughput sequencing facilitates the identification of key genes and pathways for fiber development and cell differentiation and elongation. LncRNA is a type of ncRNA with more than 200 nt in length and functions in the ways of chromatin modification, transcriptional and post-transcriptional modification, and so on. However, the detailed lncRNA and associated mechanisms for fiber initiation are still unclear in cotton. Results In this study, we used a novel glabrous mutant ZM24fl, which is endowed with higher somatic embryogenesis, and functions as an ideal receptor for cotton genetic transformation. Combined with the high-throughput sequencing, fatty acid pathway and some transcription factors such as MYB, ERF and bHLH families were identified the important roles in fiber initiation; furthermore, 3,288 lncRNAs were identified, and some differentially expressed lncRNAs were also analyzed. From the comparisons of ZM24_0 DPA vs ZM24_-2 DPA and fl_0 DPA vs ZM24_0 DPA, one common lncRNA MSTRG 2723.1 was found that function upstream of fatty acid metabolism, MBY25-mediating pathway, and pectin metabolism to regulate fiber initiation. In addition, other lncRNAs MSTRG 3390.1, MSTRG 48719.1, and MSTRG 31176.1 were also showed potential important roles in fiber development; and the co-expression analysis between lncRNAs and targets showed the distinct models of different lncRNAs and complicated interaction between lncRNAs in fiber development of cotton. Conclusions From the above results, a key lncRNA MSTRG 2723.1 was identified that might mediate some key genes transcription of fatty acid metabolism, MYB25-mediating pathway, and pectin metabolism to regulate fiber initiation of ZM24 cultivar. Co-expression analysis implied that some other important lncRNAs (e.g., MSTRG 3390.1, MSTRG 48719.1, and MSTRG 31176.1) were also showed the different regulatory model and interaction between them, which proposes some valuable clues for the lncRNAs associated mechanisms in fiber development.

show abstract

Section: Discussionsupporting

confidence: 89%

RNA-Seq with a novel glabrous-ZM24fl reveals some key lncRNAs and the associated targets in fiber initiation of cotton

et al. 2022

Self Cite

View full text Add to dashboard Cite

show abstract

“…A previous study showed that the phenotypic changes of kcs1-1 mutants included relatively thin stems and reduced resistance to low-humidity stress at a young age 56 . Moreover, a recent study showed that the transcription of most lipid biosynthesis genes, such as KCS6 , increases during the rapid elongation stage and is maintained at a high level; moreover, the fatty acid biosynthetic pathway is also significantly induced in AKR2A-57 fibres 57 . In the present study, KCS-6 accumulation was downregulated, which indicated that tea plants could improve drought resistance by inhibiting the accumulation of KCS-6.…”

Section: Discussionmentioning

confidence: 99%

Drought stress triggers proteomic changes involving lignin, flavonoids and fatty acids in tea plants

Wang

Xie

et al. 2020

Sci Rep

View full text Add to dashboard Cite

Drought stress triggers a series of physiological and biochemical changes in tea plants. It is well known that flavonoids, lignin and long-chain fatty acids play important roles in drought resistance. However, changes in proteins related to these three metabolic pathways in tea plants under drought stress have not been reported. We analysed the proteomic profiles of tea plants by tandem mass tag and liquid chromatography-tandem mass spectrometry. A total of 4789 proteins were identified, of which 11 and 100 showed up- and downregulation, respectively. The proteins related to the biosynthesis of lignin, flavonoids and long-chain fatty acids, including phenylalanine ammonia lyase, cinnamoyl-CoA reductase, peroxidase, chalcone synthase, flavanone 3-hydroxylase, flavonol synthase, acetyl-CoA carboxylase 1,3-ketoacyl-CoA synthase 6 and 3-ketoacyl-CoA reductase 1, were downregulated. However, the contents of soluble proteins, malondialdehyde, total phenols, lignin and flavonoids in the tea plants increased. These results showed that tea plants might improve drought resistance by inhibiting the accumulation of synthases related to lignin, flavonoids and long-chain fatty acids. The proteomic spectrum of tea plants provides a scientific basis for studying the pathways related to lignin, flavonoid and long-chain fatty acid metabolism in response to drought stress.

show abstract

“…3 and 4 , Table S8). The biosynthesis of VLCFAs and their transport are required for fiber development (Hu et al 2007 ). The heterologous gene KCS of cotton expressed in Arabidopsis thaliana had promoted the elongation of stem cells (Qin et al 2007 ; Shi et al 2006 ).…”

Section: Discussionmentioning

confidence: 99%

QTL controlling fiber quality traits under salt stress in upland cotton (Gossypium hirsutum L.)

Guo

Huang

et al. 2021

Theor Appl Genet

View full text Add to dashboard Cite

Key message QTL for fiber quality traits under salt stress discerned candidate genes controlling fatty acid metabolism. Abstract Salinity stress seriously affects plant growth and limits agricultural productivity of crop plants. To dissect the genetic basis of response to salinity stress, a recombinant inbred line population was developed to compare fiber quality in upland cotton (Gossypium hirsutum L.) under salt stress and normal conditions. Based on three datasets of (1) salt stress, (2) normal growth, and (3) the difference value between salt stress and normal conditions, 51, 70, and 53 QTL were mapped, respectively. Three QTL for fiber length (FL) (qFL-Chr1-1, qFL-Chr5-5, and qFL-Chr24-4) were detected under both salt and normal conditions and explained 4.26%, 9.38%, and 3.87% of average phenotypic variation, respectively. Seven genes within intervals of two stable QTL (qFL-Chr1-1 and qFL-Chr5-5) were highly expressed in lines with extreme long fiber. A total of 35 QTL clusters comprised of 107 QTL were located on 18 chromosomes and exhibited pleiotropic effects. Thereinto, two clusters were responsible for improving five fiber quality traits, and 6 influenced FL and fiber strength (FS). The QTL with positive effect for fiber length exhibited active effects on fatty acid synthesis and elongation, but the ones with negative effect played passive roles on fatty acid degradation under salt stress.

show abstract

AKR2A participates in the regulation of cotton fibre development by modulating biosynthesis of very‐long‐chain fatty acids

Cited by 23 publications

References 51 publications

RNA-Seq with a novel glabrous-ZM24fl reveals some key lncRNAs and the associated targets in fiber initiation of cotton

RNA-Seq with a novel glabrous-ZM24fl reveals some key lncRNAs and the associated targets in fiber initiation of cotton

Drought stress triggers proteomic changes involving lignin, flavonoids and fatty acids in tea plants

QTL controlling fiber quality traits under salt stress in upland cotton (Gossypium hirsutum L.)

Contact Info

Product

Resources

About