Akkumulation von Chlorogensäure in isolierten Mesophyllzellen von Ipomoea batatas Poir.

Wernicke, W.; Kohlenbach, Hans Willy

doi:10.1016/s0044-328x(76)80019-0

Cited by 4 publications

(2 citation statements)

References 10 publications

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“…Nevertheless, isolated cells like protoplasts give the opportunity to study fundamental processes, namely for example the dedifferentiation of cells, the transition from formerly quiescent cells (Go) to dividing ones (Kohlenbach 1966, Jullien 1974 and wound reactions (Wernicke and Kohlenbach 1976). Actually protoplasts are mainly the material of choice in plant micropropagation and for crop improvement.…”

Section: Summary Remarksmentioning

confidence: 99%

Plant Tissue Culture

Laimer¹,

Rücker²

2003

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PrefaceOn February 6 th , 1902 appeared Gottlieb Haberlandts publication on "Culturversuche mit isolierten Pflanzenzellen" by the Imperial Academy of Sciences in Vienna, Austria. This paper contains Haberlandt's vision on the totipotency of plant cells, an idea with worldwide dimensions representing the actual beginning of tissue culture. When Haberlandt started culturing isolated plant cells in artificial nutrient media, he was mainly interested in cell to cell relationships within a complex multicellular organism. Haberlandt had studied the physiology of the plant tissues for a long time, and such profound dedication allowed him to understand the connection between physiology and anatomy. On that basis he has been able to make such a daring vision.In fact, it was only in 1912 that A Carrel succeeded in establishing the first animal cell cultures -which was awarded a Nobel prize -and in 1934 and 1939 respectively, the first plant tissue cultures were achieved by P.A White, R.J. Gautheret and P. Nobecourt. Commercial applications of plant tissue culture followed only in the seventies. We consider this an excellent example for the time frame required from a vision to its realisation and further to its development and broad range application.We take this anniversary also as an opportunity to remind all of us, that for developing ideas time is needed together with right conditions and atmosphere. Universities in the past used to provide, what now we are risking to lose with our hectic life style and short-term planning.This book pays homage to a great Austrian scientist, describing his life and his work and the further development, success and application of his idea. Most students and scientists relate the name Haberlandt with the first lectures of plant tissue culture. But only few are acquainted with the original pieces of work, which apart from their scientific interest are true artistic masterpieces. That is why the first part of the book contains a facsimile of the original paper and its first translation into English by AD. Krikorian and D.L. Berquam in 1969. Furthermore we find it worthwhile to pay some closer attention to the way experiments were planned, carried out and evaluated by Haberlandt in his time.It was also our intention to show the development which the initial idea has made in the past spreading into the various methods and fields of application. The second and third part of the book describe Haberlandt's life and work and historical aspects of the development of plant tissue culture in the beginnings. Who could give a better glance into the early days of plant tissue culture than R.J. Gautheret? It was from his laboratory in Paris that the senior editor brought this technique and knowledge to Vienna and established this field of Preface research here in Austria. The forth part of the book contains an overview of important topics of plant tissue culture representing the most promising areas of application at present time and giving an outlook into the future. The areas range from micropropagati...

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Section: Summary Remarksmentioning

confidence: 99%

Plant Tissue Culture

Laimer¹,

Rücker²

2003

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“…The content ranges from ca 200 fmol to 5 pmol (cf. Kojima et al 1979, Moskowitz and Hrazdina 1982, Niemann et al 1983, Oba et al 1981, Wernicke and Kohlenbach 1976.…”

Section: Quantitation Of Flavonoidsmentioning

confidence: 99%

Flavonol content of guard cell and mesophyll cell protoplasts isolated from Vicia faba leaves

Weissenböck

Schnabl

Sachs

et al. 1984

Physiologia Plantarum

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O. 1984. Flavonol content of guard cell and mesophyll cell protoplasts isolated from Vicia faba leaves. -Physiol. Plant. 62: 356-362.Guard cells of the lower epidermis of leaflets of Vicia faba L. cv. WeiBkernige Hangdown contain several kaempferol 3,7-O-glycosides. This was demonstrated for the first time by the use of isolated, highly purified guard cell protoplasts for flavonol estimation and quantitation. From a total of ca 12 kaempfero! glycosides, three were identified by comparative thin layer chromatography and high perfonnance liquid chromatography as kaempferol 3-O-glucoside 7-O-rhamnoside (major component), 3-0-rhamnogalactoside 7-O-rhamnoside and 3,7-O-bisglucoside (minor components). On average, the total flavonol content was estimated to be 85 fmol protoplast-'. From comparative investigations including alkaline-induced (green) fluorescence characteristics of flavonols and UV-microscopicai studies we suggest that kaempferol glycosides are present in guard ceils and epidermal cells in simiiar quantities, and that these compounds are in the vacuole. By contrast, mesophyll protoplasts have a iow flavonol content (one sixth that of guard cells). In spite of the different total flavonoi contents, individual components of each cell-type arc the same. However, they show differences in their quantitative distribution.Additional key words -Guard ceii protoplasts, kaempferol glycosides, mesophyll protoplasts, tissue distribution.C Weissenbock (reprint requests} and G, Sachs, Botanisches Inst. Spectrum suggests the presence of flavonoid or relatedReceived

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The developmental potentials of isolated mesophyll cells and protoplasts

Kohlenbach¹

2003

Plant Tissue Culture

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Akkumulation von Chlorogensäure in isolierten Mesophyllzellen von Ipomoea batatas Poir.

Cited by 4 publications

References 10 publications

Plant Tissue Culture

Plant Tissue Culture

Flavonol content of guard cell and mesophyll cell protoplasts isolated from Vicia faba leaves

The developmental potentials of isolated mesophyll cells and protoplasts

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