Akkermansia muciniphila exerts immunomodulatory and anti-inflammatory effects on gliadin-stimulated THP-1 derived macrophages

Molaaghaee-Rouzbahani, Sara; Asri, Nastaran; Sapone, Anna; Baghaei, Kaveh; Yadegar, Abbas; Amani, Davar; Rostami-Nejad, Mohammad

doi:10.1038/s41598-023-30266-y

Cited by 9 publications

(2 citation statements)

References 39 publications

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“…Faecalibacterium and Streptococcus inhibits the production of proinflammatory cytokines by macrophages [48,49]. Akkermansia enhances the anti-inflammatory type of macrophages [50], and Prevotella treatment reduces the macrophage M1/M2 ratio [51]. These results suggest that SZEK regulates hematopoiesis through the intestinal flora, which may be associated with macrophage differentiation.…”

Section: Discussionmentioning

confidence: 88%

Impact of macrophage differentiation on hematopoietic function enhancement by Shenzhu ErKang Syrup

Li,

Teng,

Yang

et al. 2024

Aging

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Shenzhu Erkang Syrup (SZEK) is a traditional Chinese medicine that improves spleen and stomach function, tonifying the Qi and activating the blood; however, its therapeutic effects in hematopoietic dysfunction and their underlying mechanism remain unexplored. In this study, mice were given cyclophosphamide (100 mg/kg) by intraperitoneal injections for three days to produce hematopoietic dysfunction model. We investigated the hematopoietic effect and mechanism of SZEK in mice with hematopoietic dysfunction via histopathological examination, flow cytometry, enzyme-linked immunosorbent assay, and Western blotting combined with intestinal flora and serum metabolomics analysis. In mice with hematopoietic dysfunction, SZEK (gavage, 0.3 mL/25 g) alleviated pathological damage to the bone marrow and spleen; increased the number of naïve cells (Lin − ), hematopoietic stem cells (Lin − Sca-1 + c-Kit + ), long-term self-renewing hematopoietic stem cells (Lin − Sca-1 + c-Kit + CD48 − CD150 + ), B lymphocytes (CD45 + CD19 + ), and macrophages (CD11b + F4/80 + ) in the bone marrow; and reduced inflammation. Preliminary intestinal flora and serum metabolome analyses indicated that the prohematopoietic mechanism of SZEK was associated with macrophage differentiation. Further validation revealed that SZEK promoted hematopoiesis by decreasing the number of M2 macrophages and inhibiting the secretion of negative hematopoietic regulatory factors in mice with hematopoietic dysfunction.

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Section: Discussionmentioning

confidence: 88%

Impact of macrophage differentiation on hematopoietic function enhancement by Shenzhu ErKang Syrup

Li,

Teng,

Yang

et al. 2024

Aging

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“…In fact, in Fig. 4 panel A , we observed a decrease in TRAFD1 mRNA amount in LPS-stimulated cells [ 11 , 15 ]. Moreover, we verified that these changes in TRAFD1 expression were associated with the reduction of FLN29 protein synthesis, as indicated in panel B .…”

Section: Resultsmentioning

confidence: 99%

Changes upon the gluten-free diet of HLA-DQ2 and TRAFD1 gene expression in peripheral blood of celiac disease patients

Laezza,

Pisapia,

Toro

et al. 2024

Journal of Translational Autoimmunity

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The role of tryptophan metabolism and tolerogenic dendritic cells in maintaining immune tolerance: Insights into celiac disease pathogenesis

Asgari,

Khodadoust,

Nikzamir

et al. 2024

Immunity Inflam & Disease

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BackgroundIn mammals, amino acid metabolism has evolved to control immune responses. Tryptophan (Trp) is the rarest essential amino acid found in food and its metabolism has evolved to be a primary regulatory node in the control of immune responses. Celiac disease (CeD) is a developed immunological condition caused by gluten intolerance and is linked to chronic small intestine enteropathy in genetically predisposed individuals. Dendritic cells (DCs), serving as the bridge between innate and adaptive immunities, can influence immunological responses in CeD through phenotypic alterations.ObjectiveThis review aims to highlight the connection between Trp metabolism and tolerogenic DCs, and the significance of this interaction in the pathogenesis of CeD.ResultsIt is been recognized that various DC subtypes contribute to the pathogenesis of CeD. Tolerogenic DCs, in particular, are instrumental in inducing immune tolerance, leading to T‐reg differentiation that helps maintain intestinal immune tolerance against inflammatory responses in CeD patients and those with other autoimmune disorders. T‐regs, a subset of T‐cells, play a crucial role in maintaining intestinal immunological homeostasis by regulating the activities of other immune cells. Notably, Trp metabolism, essential for T‐reg function, facilitates T‐reg differentiation through microbiota‐mediated degradation and the kynurenine pathway.ConclusionTherefore, alterations in Trp metabolism could potentially influence the immune response in CeD, affecting both the development of the disease and the persistence of symptoms despite adherence to a gluten‐free diet.

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Akkermansia muciniphila exerts immunomodulatory and anti-inflammatory effects on gliadin-stimulated THP-1 derived macrophages

Cited by 9 publications

References 39 publications

Impact of macrophage differentiation on hematopoietic function enhancement by Shenzhu ErKang Syrup

Impact of macrophage differentiation on hematopoietic function enhancement by Shenzhu ErKang Syrup

Changes upon the gluten-free diet of HLA-DQ2 and TRAFD1 gene expression in peripheral blood of celiac disease patients

The role of tryptophan metabolism and tolerogenic dendritic cells in maintaining immune tolerance: Insights into celiac disease pathogenesis

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