Akkermansia muciniphila Cell-Free Supernatant Improves Glucose and Lipid Metabolisms in Caenorhabditis elegans

Abstract

To explore the mechanism by which Akkermansia muciniphila cell-free supernatant improves glucose and lipid metabolisms in Caenorhabditis elegans, the present study used different dilution concentrations of Akkermansia muciniphila cell-free supernatant as an intervention for with Caenorhabditis elegans under a high-glucose diet. The changes in lifespan, exercise ability, level of free radicals, and characteristic indexes of glucose and lipid metabolisms were studied. Furthermore, the expression of key genes of glucose and lipid metabolisms was detected by qRT-PCR. The results showed that A. muciniphila cell-free supernatant significantly improved the movement ability, prolonged the lifespan, reduced the level of ROS, and alleviated oxidative damage in Caenorhabditis elegans. A. muciniphila cell-free supernatant supported resistance to increases in glucose and triglyceride induced by a high-glucose diet and downregulated the expression of key genes of glucose metabolism, such as gsy-1, pygl-1, pfk-1.1, and pyk-1, while upregulating the expression of key genes of lipid metabolism, such as acs-2, cpt-4, sbp-1, and tph-1, as well as down-regulating the expression of the fat-7 gene to inhibit fatty acid biosynthesis. These findings indicated that A. muciniphila cell-free supernatant, as a postbiotic, has the potential to prevent obesity and improve glucose metabolism disorders and other diseases.

A purified membrane protein Amuc_1098 from Akkermansia muciniphila blunts acute pancreatitis by modulation of intestinal flora and glycerophospholipid metabolism

“…Lipids were lowered and immune function was modulated by four weeks of treatment with Akkermansia muciniphila 41 . Metabolism of glucose and lipids is improved by cell-free supernatants from Akkermansia muciniphila 42 .…”

Effect of Thermal Inactivation on Antioxidant, Anti-Inflammatory Activities and Chemical Profile of Postbiotics

“…Khmaladze et al demonstrated that cell lysates of Lactobacillus reuteri DSM 17938 reduced UV-R-induced inflammatory cytokines IL-6 and IL-8 and protected the skin barrier [24]. Wu et al found that the cell-free supernatant of Akkermansia muciniphila, as a postbiotic, has the potential to prevent obesity and improve disorders of glucose metabolism [25].…”

Geroprotective potential of microbiome modulators in the Caenorhabditis elegans model

“…Therefore, increased autophagy of aging or senescent cells may have improved lifespan in L. fermentum postbiotic-fed C. elegans , as this has been studied as a mediator of lifespan extension in multiple studies and organisms [ 133 ]. Recently, cell-free supernatants of Akkermansia muciniphila extended lifespan while reducing ROS levels and oxidative damage [ 134 ]. A. muciniphila postbiotic also modulated metabolic functions, which was indicated by upregulation of glucose metabolism (i.e., gsy-1 , pygl-1 , and pyk-1 ) and lipid metabolism (i.e., acs-2 , cpt-4 , and tph-1 ) genes and, conversely, a downregulation of fat-7 , which regulates fatty acid biosynthesis [ 134 ].…”

“…Recently, cell-free supernatants of Akkermansia muciniphila extended lifespan while reducing ROS levels and oxidative damage [ 134 ]. A. muciniphila postbiotic also modulated metabolic functions, which was indicated by upregulation of glucose metabolism (i.e., gsy-1 , pygl-1 , and pyk-1 ) and lipid metabolism (i.e., acs-2 , cpt-4 , and tph-1 ) genes and, conversely, a downregulation of fat-7 , which regulates fatty acid biosynthesis [ 134 ].…”