AKAP3 degradation in sperm capacitation is regulated by its tyrosine phosphorylation

Vizel, Ruth; Hillman, Pnina; Ickowicz, Debby; Breitbart, Haim

doi:10.1016/j.bbagen.2015.06.005

Cited by 22 publications

(21 citation statements)

References 53 publications

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“…However, AKAP3 is a scaffold protein, synthesized in round sperm cells and involved in the formation of the basic structure of the fibrous sheath (35). It is localized in the main segments of the sperm flagella and the acrosome region of sperm heads, and different studies have suggested its involvement in the regulation of sperm motility, capacitation and acrosome reaction acting as a scaffold protein that assembles multiprotein signal complexes (35,36). Although AKAP3 is known to be tyrosine phosphorylated during human sperm capacitation, the agonist showed to decrease the phosphorylation levels of AKAP3 in the Ser612, a phosphosite previously described by Ficarro et al (37).…”

Section: Discussionmentioning

confidence: 99%

“…2D). AKAPs family is one of the main components of sperm fibrous sheath, and it is involved in sperm fertility by sequestering PKA as it has a putative motif for binding cAMP-dependent protein kinase A (RIIa) (16,36). In fact, this protein family may serve as a platform for the integration of cAMP and other signaling pathways by the binding with other protein kinases, protein phosphatases, ion channels and small GTP binding proteins (41).…”

Section: Molecular and Cellular Proteomics 1813 S127mentioning

confidence: 99%

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Phosphoproteomic and Functional Analyses Reveal Sperm-specific Protein Changes Downstream of Kappa Opioid Receptor in Human Spermatozoa

Urizar-Arenaza

Osinalde

Akimov

et al. 2019

Molecular & Cellular Proteomics

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The phosphoproteome downstream KOR has been studied in human spermatozoa by applying quantitative MS-based proteomics. This studies have been combined with functional approaches to analyze the role of the receptor in the sperm physiology. Results reported phosphorylation changes in spermspecific proteins as well as the inhibition of sperm motility and acrosome after the addition of U50488H, the specific ligand. Graphical Abstract Highlights• Insights into molecular mechanisms underlying GPCR in human spermatozoa.• Quantitative phosphoproteomics downstream Kappa opioid receptor in human spermatozoa.• U50488H agonist induces phosphorylation changes in sperm-specific proteins.• U50488H inhibits human sperm motility and acrosome reaction.

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Section: Discussionmentioning

confidence: 99%

Section: Molecular and Cellular Proteomics 1813 S127mentioning

confidence: 99%

Phosphoproteomic and Functional Analyses Reveal Sperm-specific Protein Changes Downstream of Kappa Opioid Receptor in Human Spermatozoa

Urizar-Arenaza

Osinalde

Akimov

et al. 2019

Molecular & Cellular Proteomics

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“…A recent paper confirmed various cancers have deleterious impact on semen quality on large-scale [297]. Although some genes and proteins related to capacitation were reported express in cancer or involved in tumorgenesis [298–300]. A recent paper demonstrated prostasomes, the main sources of cholesterol in seminal fluid and inhibit capacitation [301], can regard as a source of diagnostic biomarkers for prostate cancer [302], reminded us indirect links between capacitation and cancer.…”

Section: Capacitation Related To Assisted Reproductive Technology Andmentioning

confidence: 99%

Factors and pathways involved in capacitation: how are they regulated?

Jin

Yang

2016

Oncotarget

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In mammals, fertilization occurs via a comprehensive progression of events. Freshly ejaculated sperm have yet to acquire progressive motility or fertilization ability. They must first undergo a series of biochemical and physiological changes, collectively known as capacitation. Capacitation is a significant prerequisite to fertilization. During the process of capacitation, changes in membrane properties, intracellular ion concentration and the activities of enzymes, together with other protein modifications, induce multiple signaling events and pathways in defined media in vitro or in the female reproductive tract in vivo. These, in turn, stimulate the acrosome reaction and prepare spermatozoa for penetration of the egg zona pellucida prior to fertilization. In the present review, we conclude all mainstream factors and pathways regulate capacitation and highlight their crosstalk. We also summarize the relationship between capacitation and assisted reproductive technology or human disease. In the end, we sum up the open questions and future avenues in this field.

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“…It has been believed for a long time that the progress of the capacitation process is coincident with the increase of tyrosine-phosphorylated proteins in mammals, including cattle, [84][85][86] although the detailed functions of the tyrosinephosphorylated proteins are poorly understood in the fertilizationrelated events of bull spermatozoa. 87 This event is regulated pivotally by the intracellular cyclic adenosine monophosphate (cAMP) signaling cascades that are composed of the bicarbonate/Ca 2+ -activated adenylyl cyclase (a soluble-type of adenylyl cyclase, ADCY10), protein kinase A (PKA), protein phosphatases, and protein tyrosine kinases. 81,[88][89][90] Moreover, various ion channels and pumps play regulatory roles in the multiple changes during capacitation.…”

Section: Capacitation-related Proteinsmentioning

confidence: 99%

Protein biomarkers for male artificial insemination subfertility in bovine spermatozoa

Harayama

Minami

Kishida

et al. 2017

Reprod Medicine & Biology

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BackgroundAlthough artificial insemination (AI) technique is an established biotechnology for bovine reproduction, the results of AI (conception rates) have a tendency to decline gradually. To our annoyance, moreover, AI‐subfertile bulls have been occasionally found in the AI centers. To resolve these serious problems, it is necessary to control the sperm quality more strictly by the examinations of sperm molecules.MethodsWe reviewed a number of recent articles regarding potentials of bovine sperm proteins as the biomarkers for bull AI‐subfertility and also showed our unpublished supplemental data on the bull AI‐subfertility associated proteins.Main findingsBull AI‐subfertility is caused by the deficiency or dysfunctions of various molecules including regulatory proteins of ATP synthesis, acrosomal proteins, nuclear proteins, capacitation‐related proteins and seminal plasma proteins.ConclusionIn order to control the bovine sperm quality more strictly by the molecular examinations, it is necessary to select suitable sperm protein biomarkers for the male reproductive problems which happen in the AI centers.

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AKAP3 degradation in sperm capacitation is regulated by its tyrosine phosphorylation

Cited by 22 publications

References 53 publications

Phosphoproteomic and Functional Analyses Reveal Sperm-specific Protein Changes Downstream of Kappa Opioid Receptor in Human Spermatozoa

Phosphoproteomic and Functional Analyses Reveal Sperm-specific Protein Changes Downstream of Kappa Opioid Receptor in Human Spermatozoa

Factors and pathways involved in capacitation: how are they regulated?

Protein biomarkers for male artificial insemination subfertility in bovine spermatozoa

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