Aislamiento y caracterización de Escherichia coli O157 en productos cárnicos bovinos y medias reses en la provincia de Tucumán

Abstract

Escherichia coli O157 is an emergent pathogen associated with diarrhea, hemorrhagic colitis and hemolytic uremic syndrome. Meat products constitute an important transmission source of this microorganism. The aims of this study were to characterize E. coli O157 isolated from cattle and meat products collected from abattoirs and retail stores, to establish the clonal relatedness among regional isolates and to compare them with those in the national database. Between 2004 and 2013, 169 minced meat, 35 sausage and 216 carcass samples were analyzed. Thirteen E. coli O157 isolates were identified; 6 of which were O157:H7 and characterized as stx2c(vh-a)/eae/ehxA (n = 5) and stx2/eae/ehxA (n = 1). The 7 remaining isolates were non-toxigenic E. coli strains, and serotyped as O157:NT (n = 4), O157:NM (n = 1), O157:ND (n = 1) and O157:H16 (n = 1). The strains yielded different XbaI-PFGE patterns. Compared to the E. coli O157 isolates in the National Database, none of these patterns have been previously detected in strains of different origin in Argentina.

Characterization of Shiga‐toxin producing Escherichia coli isolated from meat products sold in San Luis, Argentina

“…In this work, two STEC strains were isolated from fresh sausage samples, in agreement with results reported for other regions of the country (Jure et al, ). Therefore, this food could represent a human health risk when appropriate cooking temperatures are not applied.…”

“…Screening of stx by PCR is useful for the presumptive diagnosis of clinical infections triggered by STEC (Parsons, Zelyas, Berenger, & Chui, ), identification of reservoirs (Brusa et al, ) and monitoring of STEC contamination in foods (Etcheverría et al, ). Likewise, considering the importance of STEC illnesses in public health, the isolation, characterization and typing of strains is highly recommended for epidemiological purposes (Jure et al, ); therefore, effective protocols for STEC isolation and identification should be applied to food samples with stx ‐positive PCR signal (U.S. Food and Drug Administration, ). The duplex PCR protocol applied in the present study yielded positive results in the search of stx ‐positive pediatric feces until a detection limit of 10 4 STEC CFU g −1 of stools (Salinas‐Ibáñez et al, ).…”

“…However, during 2015, 337 HUS cases were reported, and even though E. coli O157:H7 was the predominant serotype isolated from patients, non‐O157 STEC strains were responsible for 25.1% of STEC infections (Brusa et al, ). Consumption of undercooked beef has been identified as a major risk factor, and meat products of different animal origin have been investigated as the most probable STEC transmission vehicles in this country (Brusa et al, ; Chinen et al, ; Jure et al, ).…”

Resistencia antimicrobiana de cepas aisladas de Escherichia coli en alimentos tipo BARF para perros en Lima, 2019

“…La ausencia de positivos para la cepa O157:H7 en este estudio pudo ser distinto con la utilización de otro tipo de serotipificación, tal como la separación inmunomagnética (Jure et al, 2015), test de concentración por inmunocaptura (Marzocca et al, 2006)  El 100% de las cepas presentaron resistencia a al menos a un antimicrobiano, siendo la resistencia a la ampicilina la frecuencia más alta (68.7%, 46/67) y la frecuencia más baja correspondió a la azitromicina con un 1.5% (1/67).…”

Escherichia coli o157:h7 en carne molida comercializada en los mercados de Guayaquil

“…Los resultados obtenidos del presente trabajo de investigación son comparables a los obtenidos en Argentina en que, de 169 muestras de carne molida de bovino, tomada de diferentes mercados y analizadas, un 5.32% fueron positivas para E. coli O157:H7 y en un segundo estudio en el 2015 reporto un 7.6% muestras positivas para E. coli O157:H7 (16).…”