Aislamiento de amebas de vida libre en piscinas públicas de Santiago de Chile

Muñoz, Vı́ctor; Reyes, Hugo Sánchez; Toche, Paola; Cárcamo, Carlos; Gottlieb, B

doi:10.4067/s0717-77122003000300003

Cited by 12 publications

(10 citation statements)

References 5 publications

(1 reference statement)

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“…For instance, two Venezuelan [52] and five Brazilian [29] PAM cases have been registered. Regarding environmental isolations in this area, it has been conducted generally at genus level without assessing the diversity at the species level, as reports of Naegleria presence in public swimming pools of Santiago, Chile [53]. Thus, our molecular findings compose a large advance to broaden the knowledge on Naegleria diversity at environmental samples, mainly in the Brazilian scenario due its lack of FLA information.…”

Section: Naegleria Spp Diversity In Brazilmentioning

confidence: 99%

Isolation of <em>Naegleria spp.</em> from a Brazilian Water Source

Bellini¹,

Fonseca²,

Reyes-Batllé³

et al. 2019

Preprint

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The genus Naegleria, of free-living amoeba (FLA) group, has been investigated mainly due to its human health impact resulting in deadly infections and their worldwide distribution on freshwater systems. Naegleria fowleri, colloquially known as the “brain-eating amoeba”, is the most studied Naegleria species because it causes Primary Amoebic Meningoencephalitis (PAM) of high lethality. The assessment of FLA biodiversity is fundamental to evaluate the presence of pathogenic species and the possibility of human contamination. However, the knowledge of FLA distribution in Brazil is unknown, and to rectify this situation we present a research on identifying Naegleria spp. in the Monjolinho River, as a model study. The river is a public Brazilian freshwater source that crosses the city of São Carlos. Five distinct sampling sites were examined through limnological features, trophozoites culturing and PCR against internal transcribed spacers (ITS) regions and 5.8S rRNA sequence. The results identified N. philippinensis, N. canariensisi, N. australiensis, N.gruberi, N. dobsoni sequences, as well as a Vahlkampfia sequence. The methodology delineated here represents the first Brazilian Naegleria spp. study on a freshwater system. Our result stresses the urgency of a large scale evaluation of the presence of free-living amoebas in Brazil.

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Section: Naegleria Spp Diversity In Brazilmentioning

confidence: 99%

Isolation of <em>Naegleria spp.</em> from a Brazilian Water Source

Bellini¹,

Fonseca²,

Reyes-Batllé³

et al. 2019

Preprint

View full text Add to dashboard Cite

show abstract

“…For instance, two Venezuelan [55] and five Brazilian [30] PAM cases have been registered. One additional Naegleria species has been identified in the public swimming pools of Santiago, Chile [56].…”

Section: Naegleria Spp Diversity In Brazilmentioning

confidence: 99%

Isolation of Naegleria spp. from a Brazilian Water Source

et al. 2020

View full text Add to dashboard Cite

The genus Naegleria, of the free-living amoeba (FLA) group, has been investigated mainly due to its human health impact, resulting in deadly infections and their worldwide distribution on freshwater systems. Naegleria fowleri, colloquially known as the “brain-eating amoeba,” is the most studied Naegleria species because it causes primary amoebic meningoencephalitis (PAM) of high lethality. The assessment of FLA biodiversity is fundamental to evaluate the presence of pathogenic species and the possibility of human contamination. However, the knowledge of FLA distribution in Brazil is unknown, and to rectify this situation, we present research on identifying Naegleria spp. in the Monjolinho River as a model study. The river is a public Brazilian freshwater source that crosses the city of São Carlos, in São Paulo state, Brazil. Five distinct sampling sites were examined through limnological features, trophozoites culturing, and PCR against internal transcribed spacer (ITS) regions and 5.8S rRNA sequences. The results identified N. philippinensis, N. canariensisi, N. australiensis, N. gruberi, N. dobsoni sequences, as well as a Hartmannella sequence. The methodology delineated here represents the first Brazilian Naegleria spp. study on a freshwater system. Our results stress the urgency of a large scale evaluation of the presence of free-living amoebas in Brazil.

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“…The presence of Acanthamoeba strains in swimming pools has been documented in several studies (Rivera et al 1993, Tsvetkova et al 2004, Gianinazzi et al 2009). In Poland (Górnik and Kuzna-Grygiel 2004) and Finland (Vesaluoma et al 1995), acanthamoebae were detected in swimming pool water, and in Chile, several genera of amoebae were detected, including Acanthamoeba (Muñoz et al 2003). In Brazil, Chaves et al (1985) found acanthamoebae in swimming pools located in the city of Campo Grande, state of Mato Grosso do Sul, CentralWestern Brazil.…”

Section: Introductionmentioning

confidence: 99%

“…In the Lagoa dos Patos estuary, Southern Brazil, Sassi et al (2010) detected the presence of Acanthamoeba in 91.7% (11/12) of the isolates obtained. Some authors report that these amoebae reach the pools through the water supply, by air or even by swimmers themselves (Muñoz et al 2003). In the state of Paraná, Southern Brazil, Ruthes et al (2004) diagnosed conjunctivitis caused by Acanthamoeba in four patients; one of them did not wear contact lenses but used to swim at the club weekly, which the authors suggested might have led to ocular infection with Acanthamoeba.…”

Section: Introductionmentioning

confidence: 99%

Detection and quantification of human adenovirus genomes in Acanthamoeba isolated from swimming pools

Staggemeier

Arantes

Caumo

et al. 2016

An. Acad. Bras. Ciênc.

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Acanthamoeba is the most common free-living environmental amoeba, it may serve as an important vehicle for various microorganisms living in the same environment, such as viruses, being pathogenic to humans. This study aimed to detect and quantify human adenoviruses (HAdV) in Acanthamoebas isolated from water samples collected from swimming pools in the city of Porto Alegre, Southern Brazil. Freeliving amoebae of the genus Acanthamoeba were isolated from water samples, and isolates (n=16) were used to investigate the occurrence of HAdVs. HAdV detection was performed by quantitative real-time polymerase chain reaction (qPCR). HAdVs were detected in 62.5% (10/16) of Acanthamoeba isolates, ranging from 3.24x10 3 to 5.14x10 5 DNA copies per milliliter of isolate. HAdV viral loads found in this study are not negligible, especially because HAdV infections are associated with several human diseases, including gastroenteritis, respiratory distress, and ocular diseases. These fi ndings reinforce the concept that Acanthamoeba may act as a reservoir and promote HAdV transmission through water.

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Aislamiento de amebas de vida libre en piscinas públicas de Santiago de Chile

Cited by 12 publications

References 5 publications

Isolation of <em>Naegleria spp.</em> from a Brazilian Water Source

Isolation of <em>Naegleria spp.</em> from a Brazilian Water Source

Isolation of Naegleria spp. from a Brazilian Water Source

Detection and quantification of human adenovirus genomes in Acanthamoeba isolated from swimming pools

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