Airway microbiota in children with bronchial mucus plugs caused by Mycoplasma pneumoniae pneumonia

Xu, Xuefeng; Wu, Lei; Sheng, Yuanjian; Liu, Jinling; Xu, Zhufei; Kong, Wei-Xing; Tang, Lanfang; Chen, Zhimin

doi:10.1016/j.rmed.2020.105902

Cited by 10 publications

(15 citation statements)

References 44 publications

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“…Bronchoalveolar lavage fluid (BAL) collections were based on our previous study [ 16 ]. Bronchoscopy was transnasally performed using a flexible video-bronchoscope (XP260F, Olympus, Tokyo, Japan) following general anesthesia.…”

Section: Methodsmentioning

confidence: 99%

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Follow-up study of airway microbiota in children with persistent wheezing

Shen

Chen

et al. 2021

Respir Res

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Background Increasing evidence revealed that airway microbial dysbiosis was associated with increased risk of asthma, or persistent wheezing (PW). However, the role of lung microbiota in PW or wheezing recurrence remains poorly understood. Methods In this prospective observational study, we performed a longitudinal 16S rRNA-based microbiome survey on bronchoalveolar lavage (BAL) samples collected from 35 infants with PW and 28 age-matched infants (control group). A 2-year follow-up study on these PW patients was conducted. The compositions of lower airway microbiota were analyzed at the phylum and genus levels. Results Our study showed a clear difference in lower airway microbiota between PW children and the control group. Children with PW had a higher abundance of Elizabethkingia and Rothia, and lower abundance of Fusobacterium compared with the control group. At the end of the 2-year follow-up, 20 children with PW (57.1%) experienced at least one episode of wheezing, and 15 (42.9%) did not suffer from wheezing episodes. Furthermore, PW children with recurrence also had increased abundances of Elizabethkingia and Rothia relative to those who had no recurrence. Additionally, wheezing history, different gender, and caesarean section demonstrated a greater impact in airway microbiota compositions. Conclusion This study suggests that the alterations of lower airway microbiota could be strongly associated with the development of wheezing, and early airway microbial changes could also be associated with wheezing recurrence later in life.

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Section: Methodsmentioning

confidence: 99%

“…DNA extraction and 16S rRNA gene sequencing methods were based on our previous study [ 16 ]. Briefly, total genomic DNA was extracted using a commercial kit according to the manufacturer’s instruction (DP382, Tiangen, Beijing, China).…”

Section: Methodsmentioning

confidence: 99%

Follow-up study of airway microbiota in children with persistent wheezing

Shen

Chen

et al. 2021

Respir Res

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“…Bronchoalveolar lavage fluid (BAL) collections were based on our previous studies [ 4 , 20 ]. Bronchoscopy was transnasally performed using a flexible video-bronchoscope (XP260F, Olympus) following general anesthesia.…”

Section: Methodsmentioning

confidence: 99%

Metabolomics of bronchoalveolar lavage in children with persistent wheezing

Liang

Chen

et al. 2022

Respir Res

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Background Recent studies have demonstrated the important role of metabolomics in the pathogenesis of asthma. However, the role of lung metabolomics in childhood persistent wheezing (PW) or wheezing recurrence remains poorly understood. Methods In this prospective observational study, we performed a liquid chromatography/mass spectrometry-based metabolomic survey on bronchoalveolar lavage samples collected from 30 children with PW and 30 age-matched infants (control group). A 2-year follow-up study on these PW children was conducted. Results Children with PW showed a distinct characterization of respiratory metabolome compared with control group. Children with PW had higher abundances of choline, oleamide, nepetalactam, butyrylcarnitine, l-palmitoylcarnitine, palmitoylethanolamide, and various phosphatidylcholines. The glycerophospholipid metabolism pathway was the most relevant pathway involving in PW pathophysiologic process. Additionally, different gender, prematurity, and systemic corticoids use demonstrated a greater impact in airway metabolite compositions. Furthermore, for PW children with recurrence during the follow-up period, children who were born prematurely had an increased abundance of butyrylcarnitine relative to those who were carried to term. Conclusions This study suggests that the alterations of lung metabolites could be associated with the development of wheezing, and this early alteration could also be correlated with wheezing recurrence later in life.

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“…Mycoplasma pulmonis (M. pulmonis) infection in mice is an excellent model to study the pathogenesis of mycoplasma respiratory infection [14]. A number of studies have shown patterns of imbalanced lung microbiota in children with M. pneumoniae pneumonia [15][16][17][18]. Wang et al reported that the bacterial diversity in M. pneumoniae pneumonia was lower than that in adenovirus pneumonia and tracheomalacia, and a high load of multi-drug-resistant Acinetobacter baumannii in the lung microbiota of several inpatients might be associated with the long hospitalization length [15].…”

Section: Introductionmentioning

confidence: 99%

“…A number of studies have shown patterns of imbalanced lung microbiota in children with M . pneumoniae pneumonia [15–18]. Wang et al reported that the bacterial diversity in M .…”

Section: Introductionmentioning

confidence: 99%

Biological functions of IL‐17‐producing cells in mycoplasma respiratory infection

et al. 2021

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Mycoplasmas are the smallest and simplest bacteria that lack a cell wall but have the capability of self-replication. Among them, Mycoplasma pneumoniae is one of the most common causes of community-acquired pneumonia. The hallmark of mycoplasma respiratory diseases is the persistence of lung inflammation that involves both innate and adaptive immune responses. In recent years, a growing body of evidence demonstrates that IL-17 plays an important role in respiratory mycoplasma infection, and associates with the pathologic outcomes of infection, such as pneumonitis and asthma. Numerous studies have shown that a variety of cells, in particular Th17 cells, in the lung can secrete IL-17 during respiratory mycoplasma infection. In this article, we review the biological functions of distinct IL-17-producing cells in mycoplasma respiratory infection with a focus on the effect of IL-17 on the outcomes of infection.

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Airway microbiota in children with bronchial mucus plugs caused by Mycoplasma pneumoniae pneumonia

Cited by 10 publications

References 44 publications

Follow-up study of airway microbiota in children with persistent wheezing

Follow-up study of airway microbiota in children with persistent wheezing

Metabolomics of bronchoalveolar lavage in children with persistent wheezing

Biological functions of IL‐17‐producing cells in mycoplasma respiratory infection

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