Evaluation of carbon dioxide (CO 2) sinks in forest areas of East and Southeast Asia (especially tropical regions) is important for assessing CO 2 budgets at the regional scale. To evaluate the CO 2 flux of large forest areas, we collected vertical CO 2 profiles over the forest using a CO 2 sonde and measured surface CO 2 concentrations around the forest using continuous CO 2 measurement equipment. These observations were performed over a typical northern forest (Hokkaido) in Japan, a subtropical forest island (Iriomote Island) in Japan, and a tropical forest in Borneo Island. We detected the differences in CO 2 vertical profiles between dawn and daytime, and at the upwind and downwind sites of the forests with the observational results from the CO 2 sonde. We also clarified that CO 2 concentrations during daytime at the downwind sites (affected by the forest) were systematically lower than those at the upwind sites (not affected by the forest). In contrast, CO 2 concentrations during dawn at the downwind sites were larger than those at the upwind site. We estimated the CO 2 fluxes (μmol m −2 s −1) at dawn and daytime of the forests from these observational results. The CO 2 fluxes of Borneo's forest were very large (16.5 and −37.7 at dawn and daytime, respectively), whereas the CO 2 fluxes of the forests in Hokkaido and Iriomote were lower (3.9 to 11.8 at dawn and −11.8 to −15.0 at daytime). These evaluated values were consistent with fluxes measured by the eddy-covariance method in the same region. Thus, use of the CO 2 sonde to collect observations of CO 2 vertical profiles was considered to be an effective method to verify CO 2 absorption and emission in large forest areas. This method can also be used to evaluate dynamic CO 2 absorption and emission processes in tropical forests.