Airborne dust collected from Kuwait in 1991–1992 augments peroxidation of cellular membrane lipids and enhances DNA damage

Athar, Mohammad; Iqbal, Mohammad; Beg, M. U.; Al‐Ajmi, D.; Al‐Muzaini, Saleh

doi:10.1016/s0160-4120(97)00137-2

Cited by 13 publications

(7 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Desert dust collected in Kuwait in 1991Kuwait in , 1992, and 1995 was found to cause cellular membrane and DNA damage (Athar et al, 1998). In another Kuwait study, atmospheric dust samples containing post-oil-fire pollutants were shown to inhibit host immune responses (Ezeamuzie et al, 1998).…”

Section: Health Implications Of Airborne Soils and Soil-associated Tomentioning

confidence: 99%

Dust Storms and Their Impact on Ocean and Human Health: Dust in Earth?s Atmosphere

2004

View full text Add to dashboard Cite

Satellite imagery has greatly influenced our understanding of dust activity on a global scale. A number of different satellites such as NASA's Earth-Probe Total Ozone Mapping Spectrometer (TOMS) andSea-viewing Field-of-view Sensor (SeaWiFS) acquire daily global-scale data used to produce imagery for monitoring dust storm formation and movement. This global-scale imagery has documented the frequent transmission of dust storm-derived soils through Earth's atmosphere and the magnitude of many of these events. While various research projects have been undertaken to understand this normal planetary process, little has been done to address its impact on ocean and human health. This review will address the ability of dust storms to influence marine microbial population densities and transport of soil-associated toxins and pathogenic microorganisms to marine environments. The implications of dust on ocean and human health in this emerging scientific field will be discussed.

show abstract

Section: Health Implications Of Airborne Soils and Soil-associated Tomentioning

confidence: 99%

Dust Storms and Their Impact on Ocean and Human Health: Dust in Earth?s Atmosphere

2004

View full text Add to dashboard Cite

show abstract

“…Deoxyribose oxidation was assayed by the method of Halliwell and Gutteridge 7 as described by Athar et al 35 Damage to the deoxyribose sugar moiety of DNA was assessed by determining the TBARS formed on incubating DNA with Fe-NTA and hydrogen peroxide in the . The reaction mixture was incubated for 60 min at 37°C in a shaking water bath.…”

Section: Assay Of Dna Damagementioning

confidence: 99%

Probucol as a potent inhibitor of oxygen radical-induced lipid peroxidation and DNA damage:in vitrostudies

2004

Self Cite

View full text Add to dashboard Cite

Probucol, a clinically used cholesterol lowering and antioxidant drug, was investigated for possible protection against lipid peroxidation and DNA damage induced by iron nitrilotriacetate (Fe-NTA) plus hydrogen peroxide (H2O2). Fe-NTA is a potent nephrotoxic agent and induces acute and subacute renal proximal tubular necrosis by catalyzing the decomposition of H2O2-derived production of hydroxyl radicals, which are known to cause lipid peroxidation and DNA damage. Fe-NTA is associated with a high incidence of renal adenocarcinoma in rodents. Lipid peroxidation and DNA damage are the principal manifestation of Fe-NTA induced toxicity, which could be mitigated by probucol. Incubation of renal microsomal membrane and/or calf thymus DNA with H2O2 (40 mM) in the presence of Fe-NTA (0.1 mM) induces renal microsomal lipid peroxidation and DNA damage to about 2.4-fold and 5.9-fold, respectively, as compared to control (P < 0.05). Induction of renal microsomal lipid peroxidation and DNA damage was inhibited by probucol in a concentration-dependent manner. In lipid peroxidation protection studies, probucol treatment showed a concentration-dependent inhibition (10-34% inhibition; P < 0.05) of Fe-NTA plus H2O2-induced lipid peroxidation as measured by thiobarbituric acid reacting species' (TBARS) formation in renal microsomes. Similarly, in DNA damage protection studies, probucol treatment also showed a concentration-dependent strong inhibition (36-71% inhibition; P < 0.05) of DNA damage. From these studies, it was concluded that probucol inhibits peroxidation of microsomal membrane lipids and DNA damage induced by Fe-NTA plus H2O2. However, because the lipid peroxidation and DNA damage studied here are regarded as early markers of carcinogenesis, we suggest that probucol may be developed as a cancer chemopreventive agent against renal carcinogenesis and other adverse effects of Fe-NTA exposure in experimental animals, in addition to being a cholesterol-lowering drug, useful for the control of hypercholestrolemia.

show abstract

“…The activity of S1 nuclease was assayed by estimating the acid soluble nucleotide released from DNA as a result of enzymatic digestion [22] as described by Athar et al [23]. Briefly, the reaction mixture in a total volume of 0.5 ml contained 0.25 ml calf thymus DNA (2.0 mg/ml), 0.025 ml peroxides/organic hydroperoxides (40 mM of each), enzyme S1 nuclease (100 U), which were mixed and the reaction was started by the addition of ferric nitrilotriacetate (0.1 mM).…”

Section: Assay Of S1 Nuclease Activitymentioning

confidence: 99%

“…The deoxyribose degradation was assayed by the method of Halliwell and Gutteridge [25], as described by Athar et al [23]. The damage to deoxyribose sugar moiety of DNA was assessed by determining the thiobarbituric acid reacting product formed on incubating DNA with ferric nitrilotriacetate in the presence of peroxides/ organic hydroperoxides.…”

Section: Assay Of Deoxyribose Degradationmentioning

confidence: 99%

Differential role of hydrogen peroxide and organic hydroperoxides in augmenting ferric nitrilotriacetate (Fe-NTA)-mediated DNA damage: Implications for carcinogenesis

Iqbal

Sharma

Mizote

et al. 2003

Teratog. Carcinog. Mutagen.

Self Cite

View full text Add to dashboard Cite

An iron chelate, ferric nitrilotriacetate (Fe-NTA), is a potent nephrotoxic agent, and induces acute and subacute renal proximal tubular necrosis, a consequence of the Fenton-like reaction that eventually leads to a high incidence of renal adenocarcinoma in rodents. In order to examine the possible mechanism for carcinogenic activity, we investigated the DNA damage with Fe-NTA in the presence of various peroxides/organic hydroperoxides. S1 nuclease hydrolysis and deoxyribose degradation assays were performed. Incubation of calf thymus DNA with ferric nitrilotriacetate (0.1 mM) in the presence of peroxides/organic hydroperoxides at a final concentration of 40 mM of each in phosphate buffer (0.1 M, pH 7.4) augmented DNA damage severalfold as compared to the damage caused by individual treatments. Fe-NTA in the presence of hydrogen peroxide caused DNA single-strand breaks and damage to its deoxyribose sugar moiety as measured, respectively, by S1 nuclease hydrolysis and deoxyribose degradation using calf thymus DNA. However, only deoxyribose degradation could be recorded in the presence of other peroxide/organic hydroperoxides. No DNA single-strand break was observed by this treatment. The observed differences in DNA damage by hydrogen peroxide and organic hydroperoxides/peroxide have been ascribed to the differential reactivity of DNA with hydroxyl and alkoxy/aryloxy free radicals produced, respectively, from these inorganic and organic peroxides. These studies suggest that Fe-NTA not only mediated the production of reactive oxygen species, but also catalysed the decomposition of these peroxides and organic hydroperoxides, which may cause a clastogenic change in DNA. This reactivity enhances the clastogenic activity in DNA. These changes in the DNA structure may ultimately be responsible, at least in part, for the induction of carcinogenesis in Fe-NTA-exposed animals.

show abstract

Airborne dust collected from Kuwait in 1991–1992 augments peroxidation of cellular membrane lipids and enhances DNA damage

Cited by 13 publications

References 13 publications

Dust Storms and Their Impact on Ocean and Human Health: Dust in Earth?s Atmosphere

Dust Storms and Their Impact on Ocean and Human Health: Dust in Earth?s Atmosphere

Probucol as a potent inhibitor of oxygen radical-induced lipid peroxidation and DNA damage:in vitrostudies

Differential role of hydrogen peroxide and organic hydroperoxides in augmenting ferric nitrilotriacetate (Fe-NTA)-mediated DNA damage: Implications for carcinogenesis

Contact Info

Product

Resources

About