The use of satellite remote sensing images could be a valid alternative to the classical methods of bathymetric measurements for depths less than 30 meters. In this work, several pixels corresponding to different depths are considered to numerically evaluate the relation between the water spectral response and the real depth, in the Douro River Estuary (Porto, Portugal). The main concept relies on principal components analysis, which allows for combining the information of the n available spectral bands from the image into an equal number n of principal components. The dataset is composed by an IKONOS-2 image and bathymetric values. An initial analysis was performed in order to determine the viability of the data for bathymetric study of the Douro River estuary. It was proved that it was not possible to find any direct relationship between the DNs of the IKONOS-2 image and depth values. Therefore, a simple linear regression of the bathymetric values on the IKONOS-2 image principal components was considered. A significant correlation was found between the first principal component and the real depths. In the future, the use of simultaneous data and the use of other statistical models such as decision trees may also provide important contributes to improve this methodology.