Despite providing health care to others every day, people who work in a hospital are usually ignored about their healthy issue. This study is aimed to investigate the prevalence of sick building syndrome (SBS)-related symptoms among hospital workers. All participants in this study completed questionnaires during the year 2013. Indoor air pollutants, Carbon monoxide (CO), Carbon Dioxide (CO 2 ), particulate matter (PM) and Volatile organic compounds (VOCs), as well as bacteria and fungi concentrations were recorded during sampling. Associations between SBS-related symptoms, individual characteristics, and chemical concentrations were evaluated by multiple linear regression. About 84% of the subjects suffered from at least one SBS-related symptom, among which the most frequently reported symptom was nasal symptoms, accounting for 66%. Chemicals such as CO 2 , PM, VOCs tended to be significantly associated with SBS-related symptoms. There are various effects depending on the type of chemicals present and whether the working spaces are open or confined.