Air pollution and ascorbic acid

Keller, Th.; Schwager, H.

doi:10.1111/j.1439-0329.1977.tb00603.x

Cited by 404 publications

(148 citation statements)

References 25 publications

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“…The leaves were then blotted dry and weighed to get turgid weight. Dry weight was determined after the leaves were kept at 105 C for 24 h. The ascorbic acid contents of leaves were determined by the colorimetric 2.6-dichlorophenol-indophenol (DIP)-method (Keller and Schwager, 1977). Briefly, 0.5 g of leaf sample was ground in liquid nitrogen and homogenized in 20 mL of 55.54 mM oxalic acid e 2.17 mM Na 2 EDTA extracting solution for 30 s in an ice-bath.…”

Section: Air Pollution Tolerance Indexmentioning

confidence: 99%

PAHs accumulation on leaves of six evergreen urban shrubs: A field experiment

Fellet

Poščić

Licen

et al. 2016

Atmospheric Pollution Research

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Section: Air Pollution Tolerance Indexmentioning

confidence: 99%

PAHs accumulation on leaves of six evergreen urban shrubs: A field experiment

Fellet

Poščić

Licen

et al. 2016

Atmospheric Pollution Research

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“…Estimation of chlorophyll, carotenoids, anthocyanin and flavonoid contents was done by the methods described by Rathore et al (2003). Leaf samples were homogenized in oxalic acid and NaEDTA extraction solution for ascorbic acid content determination as described by Keller and Schwager (1977). Determination of proline was performed by the ninhydrin assay using the method of Plummer (1979).…”

Section: Methodsmentioning

confidence: 99%

Interactive effects between supplemental ultraviolet-B radiation and heavy metals on the growth and biochemical characteristics of Spinacia oleracea L.

Mishra

Agrawal

2006

Braz. J. Plant Physiol.

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The impact of supplemental UV-B (sUV-B) radiation and heavy metals: Cd (68 µmol.kg -1 soil) and Ni (68 μmol.kg -1 soil) singly and in combination was studied on photosynthetic and non-photosynthetic pigments, metabolites, enzyme activities, biomass accumulation and the net primary productivity of spinach (Spinacia oleracea L. var. All Green), a common leafy vegetable plant. Both the stresses individually and in combination resulted in the reduction of photosynthetic pigments, ascorbic acid, and catalase activity, whereas a reverse trend was observed for anthocyanin, flavonoids and proline content, lipid peroxidation and peroxidase activity. The interactive effects of both the stresses were, however, less than additive. Combined treatment of sUV-B + Cd was more deleterious as compared to sUV-B + Ni and other individual treatments. Key words: Spinaicia oleracea, biomass, cadmium, enzymes, nickel, pigments, ultraviolet-B.Efeitos interativos entre radiação ultravioleta B suplementar e metais pesados no crescimento e características bioquí-micas de Spinacia oleracea L.: Estudou-se o impacto de radiação UV-B suplementar (sUV-B) e dos metais pesados Cd (68 µmol.kg -1 solo) e Ni (68 μmol.kg -1 solo), isoladamente ou em combinação, sobre pigmentos fotossintéticos e não fotossintéti-cos, metabólitos, atividades enzimáticas, acúmulo de biomassa e produtividade primária de espinafre (Spinacia oleracea L. var. All Green). Ambos estresses, individualmente ou em combinação, resultaram na redução dos pigmentos fotossintéticos, áci-do ascórbico, e atividade de catalase, sendo que uma tendência oposta foi observada para o conteúdo de antocianina, flavonói-des e prolina, peroxidação lipídica e atividade de peroxidase. Os efeitos interativos de ambos estresses foram, no entanto, menos que se fossem aditivos. O tratamento combinado de sUV-B + Cd foi o mais deletério quando comparado com sUV-B + Ni e outros tratamentos individuais.

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“…Leituras foram realizadas em espectrofotômetro (λ = 520 nm), após o acréscimo 2,5 mL de solução 2,6 diclorofenol-indofenol sal de sódio dihidratado (DCPiP) (0,02%) (primeira leitura) e 0,05 mL de ácido ascórbico 1% ao sobrenadante (segunda leitura). O conteúdo de ácido ascórbico foi estimado a partir da diferença entre o branco e o resultado da diferença dessas leituras (Keller & Schwager 1977).…”

Section: Introductionunclassified

Avaliação da sensibilidade de plantas jovens de quiabo (Abelmoschus esculentus (L.) Moench. - Malvaceae) ao ozônio

Bulbovas

Sant'Anna

Moraes

et al. 2008

Hoehnea

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-(Assessment of the sensitivity of okra (Abelmoschus esculentus (L.) Moench. -Malvaceae) to ozone). The sensitivity of Abelmoschus esculentus to ozone (O 3 ) was assessed in plants exposed during four days, six hours/day, to filtered air (AF) and to AF plus 80 ppb of O 3 (AF+O 3 ) into fumigation chambers, by determining visible leaf symptoms and changes on gas exchange and on antioxidants. Visible symptoms were daily evaluated. Gas exchange and antioxidants (ascorbic acid and superoxide dismutase) were analyzed at the end of the experiment. All plants from AF+O 3 treatment showed leaf injury, characterized by interveinal reddish punctuations on the adaxial surfaces. In average, 15% of leaf injury index and 62% of severity were estimated. Net photosynthesis, stomatal conductance, transpiration rate, ascorbic acid level and superoxide dismutase activity were significantly reduced in those plants, compared to measurements performed in plants exposed to AF only. The results suggest that Abelmoschus esculentus is sensitive to ozone, showing low levels of antixidative defenses and physiological distubances. Key words: Abelmoschus esculentus, antioxidants, gas exchange, ozone, visible foliar symptoms RESUMO -(Avaliação da sensibilidade de plantas jovens de quiabo (Abelmoschus esculentus (L.) Moench. -Malvaceae) ao ozônio). A sensibilidade de Abelmoschus esculentus ao ozônio (O 3 ) foi determinada em plantas expostas por quatro dias, seis horas/dia, ao ar filtrado (AF) e ao AF enriquecido com 80 ppb de O 3 (AF+O 3 ), em câmaras de fumigação, analisando-se sintomas foliares visíveis e alterações nas trocas gasosas e em antioxidantes. Avaliaram-se os sintomas foliares diariamente e as trocas gasosas e antioxidantes (ácido ascórbico e superóxido dismutase) ao fim do experimento. Todas as plantas em AF+O 3 apresentaram sintomas foliares, caracterizados por pontuações avermelhadas na superfície adaxial, entre as nervuras. Em média, o índice de injúria foliar foi de 15% e a severidade de 62%. Fotossíntese líquida, condutância estomática, transpiração, concentração de ácido ascórbico e atividade da superóxido dismutase foram reduzidas significativamente nestas plantas, em comparação com as mantidas sob ar filtrado. Os resultados sugerem que Abelmoschus esculentus é sensível ao O 3 , apresentando baixos níveis de defesas antioxidativas e distúrbios fisiológicos.

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Air pollution and ascorbic acid

Cited by 404 publications

References 25 publications

PAHs accumulation on leaves of six evergreen urban shrubs: A field experiment

PAHs accumulation on leaves of six evergreen urban shrubs: A field experiment

Interactive effects between supplemental ultraviolet-B radiation and heavy metals on the growth and biochemical characteristics of Spinacia oleracea L.

Avaliação da sensibilidade de plantas jovens de quiabo (Abelmoschus esculentus (L.) Moench. - Malvaceae) ao ozônio

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