Air-liquid interface culture changes surface properties of A549 cells

Öhlinger, Kristin; Kolesnik, Tatjana; Meindl, Claudia; Gallé, Birgit; Absenger-Novak, Markus; Kolb-Lenz, Dagmar; Frőhlich, Eleonore

doi:10.1016/j.tiv.2019.06.014

Cited by 37 publications

(42 citation statements)

References 41 publications

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“…TEER values of A549 cells were not measured at all time points because they were already low (around 50 Ohm/cm 2 ) at the start of the culture. The lack of tight barrier formation is in line with published data, e.g., [8].…”

Section: Effect Of Particles On Viability and Barrier Functionsupporting

confidence: 92%

“…Description of the particles as indicated by the producer together with hydrodynamic sizes and zeta potentials suspended in the exposure fluids are summarized in Table 2. Size and zeta potentials of particles suspended in DMEM w/o have been reported previously [8,24]. Zeta sizes were in the negative range for CPS20, CPS200 and PPS200 particles and positive for AMI200 particles.…”

Section: Characterization Of Polystyrene Particles (Hydrodynamic Sizesupporting

confidence: 63%

“…Depending on the subtype and the culture condition, they can form monolayers or multiple layers on Transwell membranes [7]. The more slowly proliferating subtype (doubling time of 24 h as used in this study) cultured in ALI with a minimum amount of medium in the basolateral compartment of the Transwell to reduce hydrostatic pressure induces the formation of monolayers [8]. For realistic in vitro testing, it would be good to include also other relevant cell types of the lungs.…”

Section: Introductionmentioning

confidence: 99%

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Screening for Effects of Inhaled Nanoparticles in Cell Culture Models for Prolonged Exposure

et al. 2021

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Respiratory exposure of humans to environmental and therapeutic nanoparticles repeatedly occurs at relatively low concentrations. To identify adverse effects of particle accumulation under realistic conditions, monocultures of Calu-3 and A549 cells and co-cultures of A549 and THP-1 macrophages in the air–liquid interphase culture were exposed repeatedly to 2 µg/cm2 20 nm and 200 nm polystyrene particles with different functionalization. Particle accumulation, transepithelial electrical resistance, dextran (3–70 kDa) uptake and proinflammatory cytokine secretion were determined over 28 days. Calu-3 cells showed constant particle uptake without any change in barrier function and cytokine release. A549 cells preferentially ingested amino- and not-functionalized particles combined with decreased endocytosis. Cytokine release was transiently increased upon exposure to all particles. Carboxyl-functionalized demonstrated higher uptake and higher cytokine release than the other particles in the A549/THP-1 co-cultures. The evaluated respiratory cells and co-cultures ingested different amounts and types of particles and caused small (partly transient) effects. The data suggest that the healthy cells can adapt to low doses of non-cytotoxic particles.

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Section: Effect Of Particles On Viability and Barrier Functionsupporting

confidence: 92%

Section: Characterization Of Polystyrene Particles (Hydrodynamic Sizesupporting

confidence: 63%

Section: Introductionmentioning

confidence: 99%

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Screening for Effects of Inhaled Nanoparticles in Cell Culture Models for Prolonged Exposure

et al. 2021

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“…A few other studies have also indicated larger effects in ALI compared to submerged cultures after exposure to different chemicals (not NPs), such as sodium metavanadate and carbendazim (Gohlsch et al 2019;Tollstadius et al 2019;Hu et al 2020). Additionally, Ohlinger et al (2019) have shown that culturing A549 cells at ALI increased the expression of several alveolar type II specific genes (for example SLCO2B1, ALB, CYP3A5, CFI) compared to when cultured in submerged, further indicating the advantages with ALI culture exposures, which more readily allows the effects of NPs on alveolar type II cell behavior. Future developments of ENM respiratory exposure models could also combine ALI cultures with mechanical stretch (simulating breathing) for even more realistic evaluations (Doryab et al 2019).…”

Section: Discussionmentioning

confidence: 98%

Toxicological effects of zinc oxide nanoparticle exposure: an in vitro comparison between dry aerosol air-liquid interface and submerged exposure systems

et al. 2021

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“…The values of surface tension were obtained from the ratio between the diameter ( D o ) of an initial droplet from the nozzle and the contact diameter ( D ) on the surface. [ 30,31 ]…”

Section: Methodsmentioning

confidence: 99%

All‐Inkjet‐Printed 3D Alveolar Barrier Model with Physiologically Relevant Microarchitecture

et al. 2021

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With the outbreak of new respiratory viruses and high mortality rates of pulmonary diseases, physiologically relevant models of human respiratory system are urgently needed to study disease pathogenesis, drug efficacy, and pharmaceutics. In this paper, a 3D alveolar barrier model fabricated by printing four human alveolar cell lines, namely, type I and II alveolar cells (NCI-H1703 and NCI-H441), lung fibroblasts (MRC5), and lung microvascular endothelial cells (HULEC-5a) is presented. Automated high-resolution deposition of alveolar cells by drop-on-demand inkjet printing enables to fabricate a three-layered alveolar barrier model with an unprecedented thickness of ≈10 µm. The results show that the 3D structured model better recapitulate the structure, morphologies, and functions of the lung tissue, compared not only to a conventional 2D cell culture model, as expected, but also a 3D non-structured model of a homogeneous mixture of the alveolar cells and collagen. Finally, it is demonstrated that this thin multilayered model reproduce practical tissue-level responses to influenza infection. Drop-on-demand inkjet-printing is an enabling technology for customization, scalable manufacturing, and standardization of their size and growth, and it is believed that this 3D alveolar barrier model can be used as an alternative to traditional test models for pathological and pharmaceutical applications.

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Air-liquid interface culture changes surface properties of A549 cells

Cited by 37 publications

References 41 publications

Screening for Effects of Inhaled Nanoparticles in Cell Culture Models for Prolonged Exposure

Screening for Effects of Inhaled Nanoparticles in Cell Culture Models for Prolonged Exposure

Toxicological effects of zinc oxide nanoparticle exposure: an in vitro comparison between dry aerosol air-liquid interface and submerged exposure systems

All‐Inkjet‐Printed 3D Alveolar Barrier Model with Physiologically Relevant Microarchitecture

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