Air‐drying of human leucocytes for scanning electron microscopy using the GTGO procedure

Gamliel, Haim; Gurfel, Dorit; Leizerowitz, Rachel; Polliack, Aaron

doi:10.1111/j.1365-2818.1983.tb04234.x

Cited by 50 publications

(13 citation statements)

References 4 publications

(2 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…For SEM, 5 mL cultures were harvested and fixed with glutaric dialdehyde for 24 h. Then the cells were embedded using the glutaraldehyde, tannic acid, guanidine hydrochloride, osmium tetroxide (GTGO) technique [12], coated with gold, and examined under the SEM microscope (Quanta200; FEI, Hillsboro, OR, USA). For the TEM analysis, the harvested cells were fixed with 1% glutaric dialdehyde for 24 h, separated by centrifugation for 5 min at 1000× g, then washed five times with 0.1 M phosphate buffer.…”

Section: Electron Microscopy Morphology Analysismentioning

confidence: 99%

Isolation and Characterization of a Marine Microalga for Biofuel Production with Astaxanthin as a Co-Product

Liu

Hou

et al. 2013

Energies

View full text Add to dashboard Cite

Microalgae have been considered as a promising biomass for biofuel production, but freshwater resource consumption during the scaled-up cultivation are still a challenge. Obtaining robust marine strains capable of producing triacylglycerols and high value-added metabolites are critical for overcoming the limitations of water resources and economical feasibility. In this study, a marine microalga with lipid and astaxanthin accumulation capability was isolated from Bohai Bay, China. The alga was named as Coelastrum sp. HA-1 based on its morphological and molecular identification. The major characteristics of HA-1 and the effects of nitrogen on its lipid and astaxanthin accumulations were investigated. Results indicated that the highest biomass, lipid and astaxanthin yields achieved were 50.9 g m , respectively, after cultivation for 24 days. The fatty acids of HA-1, identified in their majority as oleic acid (56.6%) and palmitic acid (25.9%), are desirable biofuel feedstocks. In addition, this alga can be harvested with simple sedimentation, achieving 98.2% removal efficiency after settling for 24 h. These results suggest that Coelastrum sp. HA-1 has several desirable key features that make it a potential candidate for biofuel production.

show abstract

Section: Electron Microscopy Morphology Analysismentioning

confidence: 99%

Isolation and Characterization of a Marine Microalga for Biofuel Production with Astaxanthin as a Co-Product

Liu

Hou

et al. 2013

Energies

View full text Add to dashboard Cite

show abstract

“…These appearances, on the other hand, might be better ascribed to the mordant activity of TA, which has been related to non-polar van der Waals forces (Simionescu & Simionescu, 1976a, b;Bird, 1977;Singley & Solursh, 1980;Gamliel et al, 1983).…”

Section: Discussionmentioning

confidence: 99%

“…1968, Art. 773) and was used for EM preparations according to Gamliel et al (1983). However, when, hydrolysates of collagen-GAGs, which were obtained using crude TA, were subjected to amino sugar analysis (see below, protocol II, for the preparation of collagen-GAG complexes), the peaks of both N-acetylglucosamine (GlcNAc) and GalNAc were found to have been overridden by contaminating peaks.…”

Section: Reagentsmentioning

confidence: 99%

“…Protocol I (for the SEM) consisted of treating purified collagen-GAGs, which had been fixed in glutaraldehyde as above, with crude 2% TA and 2% guanidine-HC1 in PBS for I h, washing with PBS, and post-fixation in 1% OsO4 in PBS for 1 h (Gamliel et al, 1983). The specimens were dehydrated in ascending ethanols, soaked in hexamethyldisilazane (Nation, 1983;Levanon & Stein, 1991, 1992, and air-dried.…”

Section: Preparation Of Collagen-gag Complexes For Em and For Amino Smentioning

confidence: 99%

“…In these studies, we treated our specimens with tannic acid (TA) subsequent to glutaraldehyde fixation, according to the GTGO method of Gamliel et al (1983), and thus succeeded in avoiding the production of surface irregularities during preparation.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Quantitative analysis of chondroitin sulphate retention by tannic acid during preparation of specimens for electron microscopy

Levanon

Stein

1995

Histochem J

View full text Add to dashboard Cite

The ability of tannic acid to enhance binding of glycosaminoglycans to purified collagen was analysed in an in vitro system using amino sugar analysis on an amino acid analyser, transmission electron microscopy, and scanning electron microscopy. Collagen was purified by digestion with trypsin, papain, and hyaluronidase. Purified collagen was incubated with hyaluronic acid or with chondroitin sulphate glycosaminoglycan and then treated with tannic acid. Tannic acid was found to enhance retention during preparation for electron microscopy of either of the glycosaminoglycans onto collagen fibres. The ability of tannic acid to enhance binding of collagen and glycosaminoglycans might explain, at least in part, its structural reinforcement effect on resected synovial joint-apposing surfaces during preparation for scanning electron microscopy.

show abstract

Effects of dibromochloropropane and ethylene dibromide on biochemical events and ultramorphology of ejaculated ram spermatozoa in vitro

Bartoov¹,

Ventura²,

Ailenberg³

et al. 1987

American J Industrial Med

View full text Add to dashboard Cite

The effects of dibromochloropropane (DBCP) and ethylene dibromide (EDB) on the function and ultrastructure of freshly ejaculated washed ram spermatozoa were assessed. These two compounds inhibited the collective motility of the sperm in a dose-dependent fashion when the sperm cells generated their energy either by mitochondrial respiration (2-deoxyglucose-treated sperm cells) or by the intact energy production system. DBCP and EDB inhibited the oxygen uptake by the sperm in a dose-dependent manner. No change in lactic acid accumulation and glucose utilization by the sperm cells was noted following DBCP and EDB addition. No change in the collective motility of the sperm was noted when DBCP or EDB were added to spermatozoa treated with the electron transfer inhibitor, antimycin A. Electron microscopy studies of sperm cells treated with DBCP revealed lesions in the plasma membrane adjacent to the acrosome and in the acrosomal membrane forming vesiculations. The inner membrane and the matrix space of the mitochondria were condensed following DBCP treatment, leaving a large mitochondrial peripheral space, compared with the control. EDB, at the concentration studied, caused no change in the ultramorphological structure of the sperm. DBCP was more potent, at least 4-fold, compared with EDB. An in vitro direct effect of DBCP and EDB on ram spermatozoa was established. It is suggested that quantitative measurements of sperm collective motility derived by different metabolic pathways can be used as an in vitro toxicological model for evaluation of toxicological and environmental factors affecting biological systems.

show abstract

Air‐drying of human leucocytes for scanning electron microscopy using the GTGO procedure

Cited by 50 publications

References 4 publications

Isolation and Characterization of a Marine Microalga for Biofuel Production with Astaxanthin as a Co-Product

Isolation and Characterization of a Marine Microalga for Biofuel Production with Astaxanthin as a Co-Product

Quantitative analysis of chondroitin sulphate retention by tannic acid during preparation of specimens for electron microscopy

Effects of dibromochloropropane and ethylene dibromide on biochemical events and ultramorphology of ejaculated ram spermatozoa in vitro

Contact Info

Product

Resources

About