AIE-active polysiloxane-based fluorescent probe for identifying cancer cells by locating lipid drops

Lipid Droplet-Specific Fluorescent Probe for In Vivo Visualization of Polarity in Fatty Liver, Inflammation, and Cancer Models

“…15−17,42−49 The polarity-sensitive property might be due to the fact that large charge separation and the excited-state energy of LD-TTP were dissipated in high-polarity media, leading to nonradiative relaxation of the excited energy to quench the fluorescence substantially. 47 Meanwhile, a good linearity between fluorescence intensities (F max ) and polarity (Δf) was obtained within a wide range from 0.258 (10% water) to 0.312 (80% water) according to the linear equation: F = 873,651.7 − 2,821,060 × Δf, (R 2 = 0.9829) (Figure 1b), confirming the high sensitivity of LD-TTP toward micropolarity changes. Moreover, LD-TTP had a remarkably large Stokes shift more than 124 nm that helps to reduce self-absorption and avoid the excitation interference.…”

“…Significantly, the fluorescence enhancement was about 278-fold, which was much more sensitive than most of the reported LD-polarity probes (Table S1 and Figure S2). − ,− The polarity-sensitive property might be due to the fact that large charge separation and the excited-state energy of LD-TTP were dissipated in high-polarity media, leading to nonradiative relaxation of the excited energy to quench the fluorescence substantially . Meanwhile, a good linearity between fluorescence intensities ( F max ) and polarity (Δ f ) was obtained within a wide range from 0.258 (10% water) to 0.312 (80% water) according to the linear equation: F = 873,651.7 – 2,821,060 × Δ f , ( R 2 = 0.9829) (Figure b), confirming the high sensitivity of LD-TTP toward micropolarity changes.…”

“…Because of its noninvasiveness, high sensitivity, superior spatiotemporal resolution, and simple operation, the fluorescence imaging technique has become a powerful tool for the real-time and in situ detection of biomolecules at subcellular levels and in vivo . − Until now, although a large number of fluorescent probes have been reported for specific imaging LDs, ,− few are polarity-sensitive. − ,− In addition, most of the polarity-sensitive probes are only limited for dynamic imaging of LDs. − Due to the lack of research on various disease models, the clear elucidation of the intrinsic relationship between diseases and LD polarity has not been really achieved. Currently, the disease model using LD polarity mainly focuses on the diagnosis of cancer at the level of cells or animal tissues/organs. ,,− To our knowledge, the visualization of LD polarity in vivo models, such as inflammation or clinical cancer patient samples, remains underexplored. Such limited disease models prevent us from in-depth understanding of the relationship between LD polarity and diseases.…”

A New Lipid‐Droplets‐Targeted Fluorescence Probe with Dual‐Reactive Sites for Specific Detection of ClO− in Living Cells

“…It has become clear that LDs are dynamic organelles, integral to cell metabolism, with a fascinating cycle of biogenesis, [7][8][9] consumption, [10] and highly associated with obesity, [11] diabetes, [12,13] inflammatory disorders, [14][15][16] and cancer. [17,18] The traditional imaging techniques, such as Raman microscopy, transmission electron microscopy, and immunofluorescence microscopy, [19][20][21] have been employed for the purpose to visualize lipid droplets, but some challenges have still been existed, such as complicated procedures and poor cellular permeability and thus may disturb the functions of cells. [22][23][24] Keeping these disadvantages in mind, in recent years, fluorescence imaging has been disclosed as a reliable tool for visualizing the localization and the dynamics of cellular compartments.…”

A water-soluble polymer fluorescent probeviaRAFT polymerization for dynamic monitoring of cellular lipid droplet levels and zebrafish imaging

“…Lipid droplets as highly dynamic organelles are mobilized not only to maintain the balance between energy supply and demand in the cell but also to buffer potentially toxic lipids, fulfilling an important role in the prevention of cytotoxicity and oxidative stress. [7][8][9][10][11][12][13][14] Therefore, a breakthrough in the study of monitoring the dynamic levels of intracellular lipid droplets will play an irreplaceable role in the prevention and therapeutic monitoring of major diseases in the future.…”