AhNRAMP1 Enhances Manganese and Zinc Uptake in Plants

Wang, Nanqi; Qiu, Wei; Dai, Jing; Guo, Xiaotong; Lu, Qiaofang; Wang, Tianqi; Li, Shiqin; Liu, Tongtong; Zuo, Yuanmei

doi:10.3389/fpls.2019.00415

Cited by 42 publications

(23 citation statements)

References 40 publications

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“…Under Mn-deficient conditions, the expression of AtNRAMP1 is moderately up-regulated, and nramp1 knockout mutants accumulate less Mn in shoots under Mn deficiency, which points to a function of this protein as a high-affinity Mn 2+ uptake transporter (Cailliatte et al, 2010). NRAMP1 orthologs in cacao, rapeseed, and peanut are also expressed in roots and complement a Mn uptake-deficient yeast strain, smf1, lacking a Mn transporter located in the plasma membrane (Meng et al, 2017;Ullah et al, 2018;Wang et al, 2019). In addition, active Mn 2+ uptake may be accomplished by the ZIP transporter AtIRT1 (Castaings et al, 2016), that is considered as the major Fe uptake transporter of dicots.…”

Section: Manganese Uptake From the Soil And Translocation To The Shootmentioning

confidence: 99%

Manganese in Plants: From Acquisition to Subcellular Allocation

et al. 2020

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Manganese (Mn) is an important micronutrient for plant growth and development and sustains metabolic roles within different plant cell compartments. The metal is an essential cofactor for the oxygen-evolving complex (OEC) of the photosynthetic machinery, catalyzing the water-splitting reaction in photosystem II (PSII). Despite the importance of Mn for photosynthesis and other processes, the physiological relevance of Mn uptake and compartmentation in plants has been underrated. The subcellular Mn homeostasis to maintain compartmented Mn-dependent metabolic processes like glycosylation, ROS scavenging, and photosynthesis is mediated by a multitude of transport proteins from diverse gene families. However, Mn homeostasis may be disturbed under suboptimal or excessive Mn availability. Mn deficiency is a serious, widespread plant nutritional disorder in dry, well-aerated and calcareous soils, as well as in soils containing high amounts of organic matter, where bio-availability of Mn can decrease far below the level that is required for normal plant growth. By contrast, Mn toxicity occurs on poorly drained and acidic soils in which high amounts of Mn are rendered available. Consequently, plants have evolved mechanisms to tightly regulate Mn uptake, trafficking, and storage. This review provides a comprehensive overview, with a focus on recent advances, on the multiple functions of transporters involved in Mn homeostasis, as well as their regulatory mechanisms in the plant's response to different conditions of Mn availability.

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Section: Manganese Uptake From the Soil And Translocation To The Shootmentioning

confidence: 99%

Manganese in Plants: From Acquisition to Subcellular Allocation

et al. 2020

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“…Influx of Fe (III)-phytosiderophore complexes; primary iron uptake from soil Curie et al (2001), Inoue et al (2009), Schaaf et al (2004) Arachis hypogaea AhNRAMP1 Fe, Zn, and Mn transporter and responsible for Fe, Zn, and Mn acquisition and distribution Wang et al (2019), Xiong et al (2012) Hordeum vulgare ZmYS1 Influx of Fe (III)-phytosiderophore complexes; primary iron uptake from soil Curie et al (2001), Inoue et al (2009), Schaaf et al (2004) Zea mays ZmYS1 Influx of Fe (III)-phytosiderophore complexes; primary iron uptake from soil Curie et al (2001), Inoue et al (2009), Schaaf et al (2004) Malus xiaojinensis…”

Section: Atnramp1mentioning

confidence: 99%

Potential of microbes in the biofortification of Zn and Fe in dietary food grains. A review

Singh

Prasanna

2020

Agron. Sustain. Dev.

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Micronutrients are essential factors for human health and integral for plant growth and development. Among the micronutrients, zinc (Zn) and iron (Fe) deficiency in dietary food are associated with malnutrition symptoms (hidden hunger), which can be overcome through biofortification. Different strategies, such as traditional and molecular plant breeding or application of chemical supplements along with fertilizers, have been employed to develop biofortified crop varieties with enhanced bioavailability of micronutrients. The use of microorganisms to help the crop plant in more efficient and effective uptake and translocation of Zn and Fe is a promising option that needs to be effectively integrated into agronomic or breeding approaches. However, this is less documented and forms the subject of our review. The major findings related to the mobilization of micronutrients by microorganisms highlighted the significance of (1) acidification of rhizospheric soil and (2) stimulation of secretion of phenolics. Plantmicrobe interaction studies illustrated novel inferences related to the (3) modifications in the root morphology and architecture, (4) reduction of phytic acid in food grains, and (5) upregulation of Zn/Fe transporters. For the biofortification of Zn and Fe, formulation(s) of such microbes (bacteria or fungi) can be explored as seed priming or soil dressing options. Using the modern tools of transcriptomics, metaproteomics, and genomics, the genes/proteins involved in their translocation within the plants of major crops can be identified and engineered for improving the efficacy of plant-microbe interactions. With micronutrient nutrition being of global concern, it is imperative that the synergies of scientists, policy makers, and educationists focus toward developing multipronged approaches that are environmentally sustainable, and integrating such microbial options into the mainframe of integrated farming practices in agriculture. This can lead to better quality and yields of produce, and innovative approaches in food processing can deliver cost-effective nutritious food for the undernourished populations.

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“…The entry of Cd into plant cells often accompanies the uptake of some essential micronutrients, such as iron (Fe), zinc (Zn), and manganese (Mn), because plant cells do not have a specific channel that binds to Cd only (Himeno et al, 2002;Verbruggen et al, 2009;Cai et al, 2019;Zou et al, 2020). The natural resistance-associated macrophage protein (NRAMP) family is one type of transporter responsible for metal uptake and translocation, such as Cd, Zn, Fe, and Mn (Cai et al, 2019;Wang et al, 2019c;Zou et al, 2020). OsNRAMP6 in rice functions as a Fe and Mn transporter (Peris-Peris et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

“…TpNRAMP3 in polish wheat transports Cd and Mn, but not Fe or Zn (Peng et al, 2018). AhNRAMP1 is responsible for Mn and Zn uptake in peanut (Wang et al, 2019c). Due to the response to metal uptake and transport, NRAMPs regulate many environmental stress responses.…”

Section: Introductionmentioning

confidence: 99%

MhNRAMP1 From Malus hupehensis Exacerbates Cell Death by Accelerating Cd Uptake in Tobacco and Apple Calli

et al. 2020

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Excessive cadmium (Cd) damages plants by causing cell death. The present study discusses the function of natural resistance-associated macrophage protein (NRAMP) on cell death caused by Cd in Malus hupehensis. MhNRAMP1 was isolated from M. hupehensis roots, and its protein was located in the cell membrane as a transmembrane protein characterized by hydrophobicity. MhNRAMP1 expression in the roots was induced by Cd stress and calcium (Ca) deficiency. MhNRAMP1 overexpression increased Cd concentration in yeasts and enhanced their sensitivity to Cd. Phenotypic comparisons of plants under Cd stress revealed that the growth of transgenic tobacco and apple calli overexpressing MhNRAMP1 was worse than that of the wild type (WT). The Cd 2+ influx of transgenic tobacco roots and apple calli was higher, and the recovery time of the Cd 2+ influx to a stable state in transgenic apple calli was longer than that of the WT. Cd accumulation and the percentage of apoptotic cells in transgenic lines were higher. Correspondingly, the caspase-1-like and vacuolar processing enzyme (VPE) activities and MdVPEg expression were higher in transgenic apple calli, but the expression levels of genes that inhibit cell death were lower than those in the WT under Cd stress. Moreover, the Cd translocation from the roots to leaves was increased after MhNRAMP1 overexpression, but the Cd translocation from the leaves to seeds was not affected. These results suggest that MhNRMAP1 exacerbated Cd-induced cell death, which was accomplished by mediating Cd 2+ uptake and accumulation, as well as stimulating VPE.

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AhNRAMP1 Enhances Manganese and Zinc Uptake in Plants

Cited by 42 publications

References 40 publications

Manganese in Plants: From Acquisition to Subcellular Allocation

Manganese in Plants: From Acquisition to Subcellular Allocation

Potential of microbes in the biofortification of Zn and Fe in dietary food grains. A review

MhNRAMP1 From Malus hupehensis Exacerbates Cell Death by Accelerating Cd Uptake in Tobacco and Apple Calli

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