Agronomic Traits and Molecular Marker Identification of Wheat–Aegilops caudata Addition Lines

Gong, Wenping; Han, Ran; Li, Haosheng; Jiang, Song; Yan, Huihuang; Li, Genying; Liu, Aifeng; Cao, Xinyou; Guo, Jun; Zhai, Shengnan; Cheng, Dungong; Zhao, Zhihui; Liu, Cheng; Liu, Jing

doi:10.3389/fpls.2017.01743

Cited by 35 publications

(41 citation statements)

References 41 publications

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“…caudata in a wheat background. More recently, Gong et al (2017) developed EST-based markers and Niu et al (2018) developed SSR markers for the detection of Ae. caudata chromatin in a wheat background.…”

Section: Discussionmentioning

confidence: 99%

“…The variation observed in the results indicates the presence of genomic rearrangements. Recently, studies by Danilova et al (2017), Gong et al (2017) and Niu et al (2018) on the Alcedo- Ae. caudata additions lines found that the C-genome chromosomes carried several inversions and translocations but did not agree on the rearrangements carried by each chromosome.…”

Section: Discussionmentioning

confidence: 99%

“…caudata addition lines for agronomic traits such as resistance to rust and powdery mildew diseases. Addition lines D (6C), F (3C), and G (4C) showed some resistance to powdery mildew isolates (Niu et al, 2018) while addition line E (7C) showed a higher resistance to powdery mildew (Gong et al, 2017; Niu et al, 2018). Gong et al (2017) suggested chromosome D (6C) carried leaf rust resistance while Niu et al (2018), suggested that chromosome B (2C) exhibited leaf rust resistance at a level comparable to the Ae.…”

Section: Discussionmentioning

confidence: 99%

“…Ae. caudata carries resistance genes for several diseases (Makkouk et al, 1994), especially against stripe rust (Baldauf et al, 1992; Toor et al, 2016; Valkoun et al, 1985), leaf rust (Gong et al, 2017; Iqbal et al, 2007; Riar et al, 2012), stem rust (Dyck et al, 1990; Gong et al, 2017) and powdery mildew (Baldauf et al, 1992; Gill et al, 1985; Valkoun et al, 1985; Weidner et al, 2012) and resistance against pests such as greenbug and hessian fly (Baldauf et al, 1992; Gill et al, 1985). Moreover, some accessions of Ae.…”

Section: Introductionmentioning

confidence: 99%

“…Alcedo)- Ae. caudata addition lines B-G (Schubert and Blüthner, 1992; Schubert and Blüthner, 1995) have also been characterised extensively in previous studies using cytogenetic markers (Friebe et al, 1992), isozyme analysis (Schmidt et al, 1993), Simple Sequence Repeat (SSR) markers (Gong et al, 2017; Niu et al, 2018; Peil et al, 1998), FISH with cDNA probes (Danilova et al, 2017), Conserved Orthologous Sequence (COS) and PCR-based Landmark Unique Gene (PLUG) markers (Gong et al, 2017), and sequential FISH and genomic in situ hybridization (GISH) (Niu et al, 2018). Many of these studies found that the Alcedo- Ae.…”

Section: Introductionmentioning

confidence: 99%

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Development of Wheat-Aegilops caudataIntrogression Lines and Their Characterisation Using Genome-Specific KASP Markers

Grewal

Othmeni

Walker

et al. 2020

Preprint

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22Aegilops caudata L. [syn. Ae. markgrafii (Greuter) Hammer], a diploid wild relative of wheat 23 (2n = 2x = 14, CC), is an important source for new genetic variation for wheat improvement 24 due to a variety of disease resistance factors along with tolerance for various abiotic stresses. 25 Its practical utilisation in wheat improvement can be facilitated through the generation of 26 genome-wide introgressions leading to a variety of different wheat-Ae. caudata recombinant 27 lines. In this study, we report the generation of nine such wheat-Ae. caudata recombinant lines 28 which were characterized using wheat genome-specific KASP (Kompetitive Allele Specific 29 PCR) markers and multi-colour genomic in situ hybridization (mcGISH). Of these, six lines 30 have stable homozygous introgressions from Ae. caudata and will be used for future trait 31 analysis. Through a combination of molecular and cytological analysis of all the recombinant 32 lines, we were able to physically map 182 KASP markers onto the seven Ae. caudata 33 chromosomes, of which 155 were polymorphic specifically with only one wheat subgenome. 34Comparative analysis of the physical positions of these markers in the Ae. caudata and wheat 35 genomes confirmed that the former had chromosomal rearrangements with respect to wheat, 36 as previously reported. These wheat-Ae. caudata recombinant lines and KASP markers 37 provide a useful genetic resource for wheat improvement with the latter having a wider impact 38 as a tool for detection of introgressions from other Aegilops species into wheat. 39 40 Introgression of Aegilops caudata into wheat 71 diversity in wheat breeding. Over the past decades, however, efforts have been made to study 72 the molecular organisation of the Ae. caudata genome and its homology with wheat 73 homoeologous groups. Friebe et al. (1992) showed that Ae. caudata has a highly asymmetric 74 karyotype distinct from the metacentric and submetacentric chromosomes of most Triticeae 75 species suggesting the distortion of chromosome collinearity compared to wheat. Fluorescence 76 in situ hybridisation (FISH) and molecular marker analysis of flow-sorted C-genome 77 chromosomes confirmed genome rearrangements (Molnár et al., 2016; Molnár et al., 2015). A 78 set of wheat (cv. Alcedo)-Ae. caudata addition lines B-G (Schubert and Blüthner, 1992; 79 Schubert and Blüthner, 1995) have also been characterised extensively in previous studies 80 using cytogenetic markers (Friebe et al., 1992), isozyme analysis (Schmidt et al., 1993), Simple 81 Sequence Repeat (SSR) markers (Gong et al., 2017; Niu et al., 2018; Peil et al., 1998), FISH 82 with cDNA probes (Danilova et al., 2017), Conserved Orthologous Sequence (COS) and PCR-83 based Landmark Unique Gene (PLUG) markers (Gong et al., 2017), and sequential FISH and 84 genomic in situ hybridization (GISH) (Niu et al., 2018). Many of these studies found that the 85 Introgression of Aegilops caudata into wheat Alcedo-Ae. caudata addition lines carried several inversions and translocations although ...

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Development of Wheat-Aegilops caudataIntrogression Lines and Their Characterisation Using Genome-Specific KASP Markers

Grewal

Othmeni

Walker

et al. 2020

Preprint

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Aegilops L.

Feldman,

Levy

2023

Wheat Evolution and Domestication

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This chapter discusses the classification of the genus Aegilops, and presents a detailed description of its sections and species. It shows the morphology, geographical distribution, ecological affinities, cytology, and cytogenetic aspects of the species. Similarly, the structure and distribution of repetitious DNA in the various species, evolution of the diploid and genome analyses of the allopolyploid species, and relationships between them and to species of Triticum, are presented. The occurrence of gametocidal (GC) genes in species of Aegilops, their mode of action, evolutionary significance, and use in the production of deletion and dissection bread wheat lines, are also being reviewed.

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Uncovering homeologous relationships between tetraploid Agropyron cristatum and bread wheat genomes using COS markers

et al. 2019

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Key messageUsing COS markers, the study reveals homeologous relationships between tetraploid Agropyron cristatum and bread wheat to support alien introgression breeding of wheat.AbstractCrested wheatgrass (Agropyron cristatum L. Gaertn.) is a wild relative of wheat that possesses many genes that are potentially useful in wheat improvement. The species comprises a complex of diploid, tetraploid and hexaploid forms. In this study, wheat–A. cristatum chromosome, telosome and translocation lines were used to characterize syntenic relationships between tetraploid A. cristatum and bread wheat. Prior to mapping COS markers, the cytogenetic stock lines were characterized for fertility and by FISH and GISH for karyotype stability. Out of 328 COS markers selected for the study, 279 consistently amplified products in tetraploid A. cristatum, and, out of these, 139 were polymorphic between tetraploid crested wheatgrass and wheat. Sixty-nine markers were found to be suitable for the detection of tetraploid A. cristatum chromosomes 1P–6P in wheat, ranging from 6 to 17 markers per chromosome. BLASTn of the source ESTs resulted in significant hits for 67 markers on the wheat pseudomolecules. Generally, COS markers of the same homeologous group were detected on similar arms in both Agropyron and wheat. However, some intragenomic duplications and chromosome rearrangements were detected in tetraploid A. cristatum. These results provide new insights into the structure and evolution of the tetraploid A. cristatum genome and will facilitate the exploitation of the wild species for introgression breeding of bread wheat.Electronic supplementary materialThe online version of this article (10.1007/s00122-019-03394-1) contains supplementary material, which is available to authorized users.

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Agronomic Traits and Molecular Marker Identification of Wheat–Aegilops caudata Addition Lines

Cited by 35 publications

References 41 publications

Development of Wheat-Aegilops caudataIntrogression Lines and Their Characterisation Using Genome-Specific KASP Markers

Development of Wheat-Aegilops caudataIntrogression Lines and Their Characterisation Using Genome-Specific KASP Markers

Aegilops L.

Uncovering homeologous relationships between tetraploid Agropyron cristatum and bread wheat genomes using COS markers

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