Agronomic characterization of autotetraploid banana plants derived from ‘Pisang Lilin’ (AA) obtained through chromosome doubling

Effects of In Vitro Polyploidization on Agronomic Characteristics and Fruit Carotenoid Content; Implications for Banana Genetic Improvement

“…Plant height and girth at flowering was not significantly affected by doubling, but 47% less suckering was observed in induced tetraploids than in diploid plants. This was in contrast to observations by Do Amaral et al (2015), who reported that plant height and pseudostem diameter increased while number of suckers decreased with polyploidization in Pisang Lilin. Variable changes in yield attributes following polyploidization, were also recorded.…”

“…From field observations, induced tetraploids showed distinct morphological characteristics when compared to the diploid progenitors. Induced tetraploids were characterized by long curved leaves with weak petioles resulting in a drooping leaf habit or fallen/broken leaves, as previously observed in banana (Van Duren et al, 1996; Bakry et al, 2007; Do Amaral et al, 2015). Increased leaf size following polyploidization as a result of cell division and expansion has also been reported in several other plant species, with wide applications in the improvement of leaf size and flowers of ornamental plants (Huang et al, 2015; Tamayo-Ordóñez et al, 2016; Denaeghel et al, 2018; Parsons et al, 2019).…”

“…Nyine et al (2017) did not find significant correlation between both traits in bananas when a population consisting of 307 genotypes that included diploid, triploid, and tetraploid plants was assessed. Do Amaral et al (2015) also observed fewer fruits in the tetraploid Pisang Lilin in the first cycle of evaluation, which later increased in the second cycle. Denaeghel et al (2018) also reported variable effects of polyploidization in the phenotypes of Escallonia.…”

“…Polyploid induction in Musa spp. is achieved through treatment of highly proliferating totipotent explants with anti-mitotic compounds (colchicine or oryzalin), which interfere with microtubule formation, followed by plantlet regeneration and isolation of induced tetraploids through flow cytometry (Hamill et al, 1992; Van Duren et al, 1996; Ganga and Chezhiyan, 2002; Bakry et al, 2007; Goigoux et al, 2013; Salles Pio et al, 2014; Do Amaral et al, 2015). Although there are several reports on induction of polyploidization in banana aiming to generate tetraploid breeding lines towards triploid breeding, most studies are limited to the establishment of in vitro polyploidization systems for specific cultivars and isolation of induced tetraploids.…”

Recent advances in banana (musa spp.) biofortification to alleviate vitamin A deficiency

“…The main strategy adopted for conventional banana breeding involves crossing seed-fertile 3x cultivars to 2x accessions that are donors of the genes of interest, selecting 4x and 2x hybrids from intermediate products and crossing these selected hybrids to generate sterile 3x hybrids (Tenkouano et al, 2011). Alternative schemes involving polyploidization of diploid hybrids or cultivars to obtain tetraploids for crossing with diploid lines to generate triploids, are also pursued (Bakry et al, 2009;Do Amaral et al, 2015). The use of 2x-2x hybridizations to produce secondary 3x via unilateral sexual polyploidization and 2n pollen production has also been advocated (Oselebe et al, 2006).…”