Agromyces archimandritae sp. nov., isolated from the cockroach Archimandrita tessellata

Abstract

A Gram-stain-positive bacterial strain, designated G127ATT, was isolated as soft small white colonies from the hindgut of the cockroach Archimandrita tesselata. Examination of the complete 16S rRNA sequence mapped the strain to the genus Agromyces . The type strain with the highest pairwise similarity was Agromyces marinus H23-8T (97.3%). The genome of G127ATT was sequenced by a combination of Illumina and Nanopore methods and consisted of a single circular DNA molecule with a size of 3.45 Mb. The DNA G+C content was 71.3 mol%. A phylogenomic tree based on conserved single copy housekeeping genes, placed G127ATT among the ancestral species of the genus Agromyces , and only Agromyces atrinae P27T was found to diverge earlier than G127ATT. Genome distance metrics average nucleotide identity (ANI) (76–78 %) and digital DNA–DNA hybridization (dDDH) (20.2–21.5 %) of the isolate against available genomes of several type strains of species of the genus Agromyces indicated that G127ATT represented a previously undescribed species of the genus Agromyces . Morphological, physiological and biochemical characteristics, including lipid profile, cellular fatty acids and peptidoglycan type were in accordance with usual attributes of members of the genus Agromyces . The novel isolate could be differentiated from the most closely related species by extracellular expression of acid and alkaline phosphatases, trypsin and α-chymotrypsin, and utilization of l-arabinose and salicin as sole carbon sources. On the basis of the combined genomic and phenotypic features, isolate G127ATT (=DSM 111850T=LMG 32099T) is considered to represent a novel species of the genus Agromyces , for which we propose the name Agromyces archimandritae sp. nov.

Vagococcus luciliae sp. nov., isolated from the common green bottle fly Lucilia sericata

“…The genome of G314F T was sequenced and assembled into two circular contigs by a combination of short-and long-read sequencing technologies as described previously [23,32]. Briefly, DNA was extracted by following a modified Marmur protocol [33], and used for Illumina library preparation with the Nextera XT DNA sample preparation kit (Illumina).…”

Agromyces larvae sp. nov., a novel actinobacterium isolated from gut of larvae of Protaetia brevitarsis seulensis

“…One of the species, namely Agromyces mediolanus , has recently been reported to be infectious to humans [14]. In recent times, a few Agromyces species have been isolated from insect guts [4, 6, 15]. Insects, in general, have been historically documented to be consumed by humans and are currently considered as an efficient, economic and environmentally friendly source of nutrients [16].…”

“…Members of the genus Agromyces including the type species Agromyces ramosus have been predominantly isolated from soil and rhizosphere region of plants [1,2]. The genus, which currently consists of 44 species with validly published names [2], hosts members which are characteristically Gram-positive, non-spore-forming, aerobic to micro-aerophilic, filamentous or nonfilamentous, contain B type peptidoglycan, anteiso-C 15 : 0 , anteiso-C 17 : 0 and iso-C 16 : 0 as the major fatty acids, and MK-11 and K-12 as the predominant menaquinones [1,[3][4][5][6][7][8]. Though several species of Agromyces have been isolated from soil and plant rhizosphere, the origins of several other members are ecologically diverse, including mangrove ecosystem, caves, marine environment and foods, Roman catacombs, animals and insects [4,5,[9][10][11][12][13].…”

The resuscitation-promoting factor (Rpf) from Micrococcus luteus and its putative reaction product 1,6-anhydro-MurNAc increase culturability of environmental bacteria

“…The addition of Rpf to isolation media has been used to improve the recovery of bacteria from environmental samples, resulting in the cultivation of novel species of Actinobacteria [16][17][18][19]. Moreover, Rpf enhances biodegradation of noxious contaminants by environmental bacteria [20,21].…”