Agroinfiltration-based expression of hairpin RNA in soybean plants for RNA interference against Tetranychus urticae

Dubey, Vimal Kumar; Lee, Ung Gyu; Kwon, Deok Ho; Lee, Si Hyeock

doi:10.1016/j.pestbp.2017.01.004

Cited by 12 publications

(7 citation statements)

References 25 publications

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“…A feeding delivery method with multi‐unit chambers was used to screen multiple target genes for RNAi toxicity, and of the 42 genes tested, coatomer subunit epsilon was one of the best candidate target genes with the highest toxicity (LT 50 = 89.7 h) . Subsequently, hairpin dsRNA against this candidate gene from T. urticae was transiently expressed in soybean plants by agroinfiltration, and indeed there was a significant increase in the mortality of the mites after feeding on the transgenic soybean plants . In addition, dsRNA was also used to silence different detoxification‐related genes (e.g.…”

Section: Efficiency Of Rna In Various Mite Speciesmentioning

confidence: 99%

“…27,28 Subsequently, hairpin dsRNA against this candidate gene from T. urticae was transiently expressed in soybean plants by agroinfiltration, and indeed there was a significant increase in the mortality of the mites after feeding on the transgenic soybean plants. 29 In addition, dsRNA was also used to silence different detoxification-related genes (e.g. esterase and NADPH-cytochrome P450 reductase) in T. cinnabarinus with a gene-silencing efficiency ranging between 47 and 65%.…”

Section: Gene Silencing and Phenotypic Effects Induced By Dsrna In MImentioning

confidence: 99%

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Beyond insects: current status and achievements of RNA interference in mite pests and future perspectives

Niu

Shen

Christiaens

et al. 2018

Pest Management Science

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Mites comprise a group of key agricultural pests on a wide range of crops. They cause harm through feeding on the plant and transferring dangerous pathogens, and the rapid evolution of pesticide resistance in mites highlights the need for novel control methods. Currently, RNA interference (RNAi) shows great potential for insect pest control. Here, we review the literature regarding RNAi in mite pests. We discuss different target genes and RNAi efficiency in various mite species, a promising Varroa control program using RNAi, the synergy of RNAi with plant defense mechanisms and microorganisms, and current understanding of systemic movement of double-stranded RNA (dsRNA). On the basis of this evidence, we can conclude that there is clear potential for application of RNAi-based mite control, but further research on several aspects of RNAi in mites is needed, including: (i) the factors influencing RNAi efficiency, (ii) the mechanism of environmental RNAi and cross-kingdom dsRNA trafficking, (iii) the mechanism of possible systemic and parental RNAi, and (iv) non-target effects, specifically in predatory mites, which should be considered during RNAi target selection. © 2018 Society of Chemical Industry.

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Section: Efficiency Of Rna In Various Mite Speciesmentioning

confidence: 99%

Section: Gene Silencing and Phenotypic Effects Induced By Dsrna In MImentioning

confidence: 99%

Beyond insects: current status and achievements of RNA interference in mite pests and future perspectives

Niu

Shen

Christiaens

et al. 2018

Pest Management Science

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“…It is possible that: (i) the targets selected were not lethal genes. The screening of a large number of candidate genes by RNAi could greatly enhance the mortality and promote the application of RNAi‐based pest control . Based on the currently sequenced aphid genomes, there are approximately 10 249 to 19 182 genes; therefore, our test of only seven genes represents a tiny fraction of the whole gene set that could be explored.…”

Section: Discussionmentioning

confidence: 99%

Topical dsRNA delivery induces gene silencing and mortality in the pea aphid

Niu

Yang

Tian

et al. 2019

Pest Management Science

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BACKGROUND: With the growing number of available aphid genomes and transcriptomes, an efficient and easy-to-adapt tool for gene function study is urgently required. RNA interference (RNAi), as a post-transcriptional gene silencing mechanism, is important as a research tool for determining gene functions and has potential as a novel insect control strategy. However, these applications have been hampered by the lack of effective dsRNA delivery approaches in aphids. RESULTS:Here, we developed a convenient and efficient dsRNA delivery method, topical RNAi, in aphids. An investigation of its dose and time-dependent RNAi efficiencies revealed that with as little as 60 ng dsRNA per adult pea aphid (Acyrthosiphon pisum), the indicator gene, Aphunchback, could be significantly silenced within 2 h of exposure. The method was further validated by successfully silencing other different genes, and it was also efficient toward two other aphid species, Aphis citricidus and Myzus persicae. Furthermore, a noticeable mortality was also observed in pea aphids using topical RNAi-mediated gene silencing, within 4 days post-dsRNA application for four out of seven tested genes. CONCLUSION: Compared with the currently used dsRNA delivery methods in aphids, microinjection and ingestion, topical RNAi is time-and cost-effective, which could greatly influence RNAi-based gene functional studies and potential candidate gene selection for developing RNAi-based aphid control strategies in the future.

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“…Agroinfiltration is also applicable to effectoromics field involving rapid resistance and a virulence gene discovery [ 212 ]. Agroinfiltration has played a major role in a variety of studies including the gene silencing [ 122 , 213 , 214 , 215 , 216 , 217 ], elicitor identification [ 218 , 219 ], resistance mechanism [ 220 , 221 , 222 , 223 ], promoter characterization [ 224 , 225 ], expression studies [ 226 ], identification of plant receptor(s) [ 227 ], antigen-antibody interactions [ 228 , 229 , 230 , 231 ], vaccine production, protein ubiquitination [ 232 ], protein degradation [ 233 ], pigment [ 209 ] and phyto-sensing studies [ 234 ]. It has the potential to give a scrutinizing view of the plant system efficiently in the present times.…”

Section: Other Applications In Plantsmentioning

confidence: 99%

Agroinfiltration Mediated Scalable Transient Gene Expression in Genome Edited Crop Plants

Kaur

Manchanda

Kalia

et al. 2021

IJMS

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Agrobacterium-mediated transformation is one of the most commonly used genetic transformation method that involves transfer of foreign genes into target plants. Agroinfiltration, an Agrobacterium-based transient approach and the breakthrough discovery of CRISPR/Cas9 holds trending stature to perform targeted and efficient genome editing (GE). The predominant feature of agroinfiltration is the abolishment of Transfer-DNA (T-DNA) integration event to ensure fewer biosafety and regulatory issues besides showcasing the capability to perform transcription and translation efficiently, hence providing a large picture through pilot-scale experiment via transient approach. The direct delivery of recombinant agrobacteria through this approach carrying CRISPR/Cas cassette to knockout the expression of the target gene in the intercellular tissue spaces by physical or vacuum infiltration can simplify the targeted site modification. This review aims to provide information on Agrobacterium-mediated transformation and implementation of agroinfiltration with GE to widen the horizon of targeted genome editing before a stable genome editing approach. This will ease the screening of numerous functions of genes in different plant species with wider applicability in future.

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Agroinfiltration-based expression of hairpin RNA in soybean plants for RNA interference against Tetranychus urticae

Cited by 12 publications

References 25 publications

Beyond insects: current status and achievements of RNA interference in mite pests and future perspectives

Beyond insects: current status and achievements of RNA interference in mite pests and future perspectives

Topical dsRNA delivery induces gene silencing and mortality in the pea aphid

Agroinfiltration Mediated Scalable Transient Gene Expression in Genome Edited Crop Plants

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