Agrococcus versicolor sp. nov., an actinobacterium associated with the phyllosphere of potato plants

Abstract

The taxonomic position of a group of actinobacterial strains isolated from the phyllosphere of potato plants was investigated by using a polyphasic approach. Although the similarity values for their 16S rRNA gene sequences suggested an intermediate position between Microbacterium and Agrococcus, the phylogenetic tree demonstrated a clear clustering of the representative strain, K 114/01 T , within the genus Agrococcus. The presence of 2,4-diaminobutyric acid as the diagnostic diamino acid in the cell-wall peptidoglycan of strain K 114/01 T substantiated the affiliation to the genus Agrococcus. An analysis performed using matrix-assisted laser desorption ionization-time-of-flight mass spectrometry revealed highly similar spectral patterns for the isolated strains, which, together with their conformity regarding a multitude of phenotypic features, supported their affiliation to the same species. Differences in several physiological features, peptidoglycan and menaquinone composition and whole-cell fatty acid profiles enabled discrimination of the phyllosphere isolates with respect to recognized Agrococcus species. As the 16S rRNA gene sequence similarity values were below 97 %, the strains isolated from the phyllosphere of potato plants represent a novel species of the genus Agrococcus, for which the name Agrococcus versicolor sp. nov. is proposed. The type strain is K 114/01 T (5DSM 19812 T 5LMG 24386 T ).

Effects of transgenic fructan-producing potatoes on the community structure of rhizosphere and phyllosphere bacteria

“…Actinobacteria are known to be typical inhabitants of the phyllosphere and could be cultivated from the leaf surface of several plant species (van Outryve et al , 1989; Ercolani, 1991; Thompson et al , 1993; Legard et al , 1994; Korsten et al , 1995; Behrendt et al , 1997). However, several isolates were assigned to the genus Agrococcus , which was identified for the first time in association with plants (Behrendt et al , 2008). It is notable that genera that comprise typical inhabitants of the phyllosphere such as Microbacterium, Curtobacterium and Plantibacter (Evtushenko & Takeuchi, 2006) were found in high abundance on the control plants but represented only a minority on the transgenic line.…”

“…Agrococcus, which was identified for the first time in association with plants (Behrendt et al, 2008). It is notable that genera that comprise typical inhabitants of the phyllosphere such as Microbacterium, Curtobacterium and Plantibacter (Evtushenko & Takeuchi, 2006) were found in high abundance on the control plants but represented only a minority on the transgenic line.…”

Lysinimonas soli gen. nov., sp. nov., isolated from soil, and reclassification of Leifsonia kribbensis Dastager et al. 2009 as Lysinimonas kribbensis sp. nov., comb. nov.

“…Table 1. Differential characteristics of closely related genera of the family Microbacteriaceae Genera: 1, Lysinimonas gen. nov.; 2, Agreia (Evtushenko et al, 2001;Behrendt et al, 2002, Schumann et al, 2003; 3, Agrococcus (Groth et al, 1996;Wieser et al, 1999;Zlamala et al, 2002;Mayilraj et al, 2006;Bora et al, 2007;Lee, 2008;Behrendt et al, 2008;Zhang et al, 2010;Dhanjal et al, 2011); 4, Chryseoglobus (Baik et al, 2010); 5, Glaciibacter (Katayama et al, 2009); 6, Herbiconiux (Qiu et al, 2007;Behrendt et al, 2011;Kim et al, 2012); 7, Labedella (Lee, 2007); 8, Leifsonia (Leifson, 1962;Davis et al, 1984;Suzuki et al, 1999;Evtushenko et al, 2000;Reddy et al, 2003;An & Yokota, 2007;Qiu et al, 2007;Dastager et al, 2008Dastager et al, , 2009Pindi et al, 2009;An et al, 2009An et al, , 2010Madhaiyan et al, 2010;Ganzert et al, 2011); 9, Microcella (Tiago et al, 2005(Tiago et al, , 2006; 10, Microterricola (Matsumoto et al, 2008); 11, Phycicola (Lee et al, 2008); 12, Rhodoglobus (Reddy et al, 2003;Sheridan et al, 2003;An et al, 2010); 13, Salinibacterium (Han et al, 2003;Zhan...…”

First Draft Genome Sequence from a Member of the Genus Agrococcus , Isolated from Modern Microbialites

“…The genus Agrococcus is classified within the family Microbacteriaceae , members of which exhibit the unusual feature of having diaminobutyric acid within the cell wall, which imparts a distinctive yellow color (1). The genus has eight described species that have been isolated from a wide range of environments, including air (2), a coal mine (3), cheese (4), cold desert soil (5), forest soil (6), a medieval wall painting (7), dried seaweed (8), and the phyllosphere of potato plants (9). The genetic richness and diversity of this genus is poorly known, with little genetic information available other than for the 16S rRNA genes.…”