Agrobacterium tumefaciens-mediated transformation of Japanese white birch (Betula platyphylla var. japonica)

Mohri, Takeshi; Mukai, Yuzuru; Shinohara, Kenji

doi:10.1016/s0168-9452(97)00107-6

Cited by 24 publications

(14 citation statements)

References 30 publications

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“…We established previously a simple and reliable procedure for the regeneration of transgenic Japanese broad-leaved trees (Mohri et al 1996(Mohri et al , 1997(Mohri et al , 1999Igasaki et al 2000). However, to our knowledge, no studies of the transformation of Japanese coniferous species have been reported.…”

Section: Resultsmentioning

confidence: 99%

Somatic embryogenesis and plant regeneration from immature zygotic embryos of Cryptomeria japonica D. Don

et al. 2003

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This report describes the successful plant regeneration via somatic embryogenesis from immature zygotic embryos of Cryptomeria japonica D. Don. For the induction of embryogenic tissue, we determined that the optimal medium contained N6-benzyladenine and 2,4-dichlorophenoxyacetic acid. Immature zygotic embryos that were collected at the end of June yielded embryogenic tissue at the highest frequency. Embryogenic tissues that had proliferated in liquid medium included small and loosely packed cells and elongating or elongated cells. We used ten cell lines to determine the optimal medium for the development of somatic embryos. Induced somatic embryos germinated with synchronous sprouting of cotyledons, hypocotyls and roots. Gibberellin A3 in the germination medium had a positive effect on both the elongation of hypocotyls and the survival of seedlings. The frequencies of induction and germination of somatic embryos differed among the cell lines examined. Most of the seedlings grew normally. This system of somatic embryogenesis required 4-5 months for the regeneration of C. japonica plantlets from immature zygotic embryos.

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Section: Resultsmentioning

confidence: 99%

Somatic embryogenesis and plant regeneration from immature zygotic embryos of Cryptomeria japonica D. Don

et al. 2003

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“…The inoculation time usually applied in transformation procedures is about 15 min for citrange epicotyl (Cervera et al 1998), 30 min for birch leaf discs (Mohri et al 1997) and for cotton hypocotyls (Chair et al 1997), and up to 1 h for Casuarina glauca epicotyls (Le et al 1996). Interestingly, Arokiaraj and co-workers reported that, in their transformation process, H. brasiliensis anther calli were submerged in an A. tumefaciens culture for only 1 min ).…”

Section: Influence Of Biotic and Abiotic Stressmentioning

confidence: 99%

Production of Hevea brasiliensis transgenic embryogenic callus lines by Agrobacterium tumefaciens: roles of calcium

et al. 2003

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A procedure has been established for Agrobacterium tumefaciens-mediated genetic transformation of Hevea brasiliensis embryogenic friable calli. Precultivation of tissues on a CaCl(2)-free maintenance medium dramatically enhanced the transient activity of the reporter gene, gusA encoding beta-glucuronidase (GUS). The increase was first noticed in highly active cells (undifferentiated or/and embryogenic), in tissues precultured for 2-8 weeks. Beyond 8 weeks of preculture, GUS activity increased again, but this time in tissues consisting of differentiated cells accumulating polyphenols. Out of five Agrobacterium strains cocultivated with CaCl(2)-free precultured tissues, only inoculation with EHA105pC2301 led to high transient GUS activity. Paromomycin proved more effective than kanamycin for the selection of transformed cells, as it inhibits the growth of non-transformed cells more radically. Five paromomycin-resistant callus lines were established. The presence of gusA and neomycin phosphotransferase ( nptII) genes in the plant genome was confirmed by DNA amplification, and by Southern hybridization. These results confirmed that A. tumefaciens is an effective system for mediating stable transformation of rubber tree calli with a low copy number of transgenes. Transgenic callus lines constitute a useful tool for studying genes of interest on a cellular level and for regenerating transgenic rubber trees.

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“…In previous studies, we established a simple and reliable procedure for the regeneration of transgenic Japanese broadleaved trees (Mohri et al 1996, Mohri et al 1997, Mohri et al 1999. However, to our knowledge, no studies of the transformation of Japanese coniferous species have been reported.…”

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confidence: 99%

Phytosulfokine Stimulates Somatic Embryogenesis in Cryptomeria japonica

Igasaki

Akashi

Ujino‐Ihara

et al. 2003

Plant and Cell Physiology

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; Phytosulfokine (PSK), which has been identified as a plant growth factor, had a dramatic stimulatory effect on the formation of somatic embryos of sugi (Cryptomeria japonica) in the presence of polyethylene glycol. The resultant somatic embryos germinated with synchronous sprouting of cotyledons, hypocotyls and roots, and most of the seedlings grew normally. A cDNA clone for the precursor to the PSK peptide of C. japonica was identified in an expressed sequence tags database. Our results support the existence of a PSK signaling pathway in C. japonica.

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Agrobacterium tumefaciens-mediated transformation of Japanese white birch (Betula platyphylla var. japonica)

Cited by 24 publications

References 30 publications

Somatic embryogenesis and plant regeneration from immature zygotic embryos of Cryptomeria japonica D. Don

Somatic embryogenesis and plant regeneration from immature zygotic embryos of Cryptomeria japonica D. Don

Production of Hevea brasiliensis transgenic embryogenic callus lines by Agrobacterium tumefaciens: roles of calcium

Phytosulfokine Stimulates Somatic Embryogenesis in Cryptomeria japonica

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