Agrobacterium tumefaciens -mediated transformation of greenhouse-grown Brassica rapa ssp. oleifera

Kuvshinov, Viktor; Koivu, Kimmo; Kanerva, Anne; Pehu, Eija

doi:10.1007/s002990050659

Cited by 38 publications

(25 citation statements)

References 15 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…It was observed from the present study that use of BAP and NAA in the medium helped in shoot regeneration. This result agreement with the findings of Kunshinov et al (1999) Cao et al, (2003, Du et al (2000), Wang et al (2000), Patil and Pillewan (2002) and Sayem (2010). They reported that for shoot regeneration BAP and NAA was the most effective stimulator.…”

Section: Effects the Of Explantssupporting

confidence: 83%

Callus Derived Regeneration of Some Selected Brassica Genotypes

et al. 2018

View full text Add to dashboard Cite

The experiment was conducted to observe the callus induction ability of Brassica species. Plantlets were regenerated from cotyledon and stem explants of Brassica napus, Brassica campestris and Brassica juncea through direct organogenesis. The experiments were conducted in a Completely Randomized Design (CRD) with 4 replications. The highest frequency of callus formation was recorded in MS containing 2.0 mgl -1 BAP, 0.5 mgl -1 NAA and 2.0 mgl -1 AgNO 3 in both stem and cotyledon explants. Among these explants, stem was found to be better responsive in callus induction than cotyledon. Among the genotypes used, BINA Sarisha-4 induced the highest percentage (100.00%) of callus from stem explants which was followed by BINA Sarisha-5 (100.00%) and Sampad (83.35%). On the other hand, BINA Sarisha-4 induced callus from 91.67% cotyledon explants, followed by BINA Sarisha-5 (75.00%) and Sampad (66.67%). Similarly, the highest percentage of shoot regeneration (58.34%) from stem explants of BINA Sarisha-4 was observed in MS medium supplemented with combination of hormone and silver nitrate concentrations. The highest percentage of root induction was 66.67 and 58.33% in plantlets derived from stem and cotyledon explants, respectively in ½ MS medium supplemented with 2.0 mgl -1 IBA and 0.5 mgl -1 of NAA. The highest survival rate was found after acclimatization of plants derived from stem (77.78%) and cotyledon (64.28%) explants of BINA Sarisha-4 in pot and 64.33 and 55.55%, respectively in field.

show abstract

Section: Effects the Of Explantssupporting

confidence: 83%

Callus Derived Regeneration of Some Selected Brassica Genotypes

et al. 2018

View full text Add to dashboard Cite

show abstract

“…FPsc plants for transformation were grown on sterile agar plates, and young cotyledon explants were transformed with Agrobacterium tumefaciens GV3101 carrying the UBQ10pro::AtPHYB construct. Hormone conditions used to generate callus and regenerate shoots were as described (Kunvshinov et al, 1999). We were unable to regenerate roots under a variety of conditions tested; however, when we transferred shoots to soil, one of these regenerated a root to provide transgenic seeds.…”

Section: Transformation Of B Rapa Fpscmentioning

confidence: 99%

Cotyledon-Generated Auxin Is Required for Shade-Induced Hypocotyl Growth in Brassica rapa

et al. 2014

View full text Add to dashboard Cite

Plant architecture is optimized for the local light environment. In response to foliar shade or neighbor proximity (low red to far-red light), some plant species exhibit shade-avoiding phenotypes, including increased stem and hypocotyl growth, which increases the likelihood of outgrowing competitor plants. If shade persists, early flowering and the reallocation of growth resources to stem elongation ultimately affect the yield of harvestable tissues in crop species. Previous studies have shown that hypocotyl growth in low red to far-red shade is largely dependent on the photoreceptor phytochrome B and the phytohormone auxin. However, where shade is perceived in the plant and how auxin regulates growth spatially are less well understood. Using the oilseed and vegetable crop species Brassica rapa, we show that the perception of low red to far-red shade by the cotyledons triggers hypocotyl cell elongation and auxin target gene expression. Furthermore, we find that following shade perception, elevated auxin levels occur in a basipetal gradient away from the cotyledons and that this is coincident with a gradient of auxin target gene induction. These results show that cotyledon-generated auxin regulates hypocotyl elongation. In addition, we find in mature B. rapa plants that simulated shade does not affect seed oil composition but may affect seed yield. This suggests that in field settings where mutual shading between plants may occur, a balance between plant density and seed yield per plant needs to be achieved for maximum oil yield, while oil composition might remain constant.

show abstract

“…Therefore, GFP can be valuable to facilitate the screening process for transgenic plants that express functional proteins, if they are fused to the target gene. This may be important especially when kanamycin (Km) is used for selection since it was recently shown that the proportion of "escapes" may be very high under Km-selection (Kuvshinov et al 1999). The gusA gene is another widely used reporter to detect transformed plants and cells.…”

Section: Pcr Analysis and Gfp Detectionmentioning

confidence: 99%

Optimization in Agrobacterium-mediated transformation of Anthurium andraeanum using GFP as a reporter

Zhao¹,

Ji²,

Wang³

et al. 2010

Electron. J. Biotechnol.

View full text Add to dashboard Cite

Although Agrobacterium-mediated transformation protocols for many economically important plant species have been well established, protocol for a number of flowering plants including Anthurium andraeanum remains challenging. In this study, we report success in generating transgenic Anthurium andraeanum cv Arizona using Agrobacterium GV3101 strain harboring a binary vector carrying gfp as a reporter gene. The possibility of facilitating the screening process for transgenic plants expressing functional proteins using gfp marker was explored. In order to realize high transformation efficiency, different explant sources including undifferentiated callus pieces and petioles were compared for their regeneration efficiency and susceptibility to Agrobacterium-mediated transformation. We also optimized the concentration of AS added to co-cultivation media. Genomic PCR revealed that 11 of the 22 resistant plantlets regenerated on selective medium were successfully transformed. Green fluorescence was observed using a fluorescence microscope in 7 of the 11 PCR-positive plants, indicating GFP was expressed stably in the transformed Anthurium andraeanum. The highest transformation efficiency obtained in this study was 1.71% (percentage of explants with transgenic shoots in total explants) when callus explants were used as starting material and 125 μmol l -1 AS was added during the co-cultivation process.

show abstract

Agrobacterium tumefaciens -mediated transformation of greenhouse-grown Brassica rapa ssp. oleifera

Cited by 38 publications

References 15 publications

Callus Derived Regeneration of Some Selected Brassica Genotypes

Callus Derived Regeneration of Some Selected Brassica Genotypes

Cotyledon-Generated Auxin Is Required for Shade-Induced Hypocotyl Growth in Brassica rapa

Optimization in Agrobacterium-mediated transformation of Anthurium andraeanum using GFP as a reporter

Contact Info

Product

Resources

About