Agrobacterium tumefaciens-mediated transformation of Coniella granati

Yuan, Huatang; Huang, Hui; Huang, Tianxiang; Zhou, Zhongli; Tu, Hongtao; Wang, Li

doi:10.1016/j.mimet.2021.106149

Cited by 5 publications

(3 citation statements)

References 31 publications

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“…As shown in Figure 1D, the yield of transformants was highest after 48 h of co-cultivation and was >30 times higher than that after 24 h of co-cultivation. Such a short co-cultivation period results in a lower number of transformation events compared to those after 36, 48, or 72 h [13,15,28,29,37,42,43].…”

Section: Discussionmentioning

confidence: 98%

“…To the best of our knowledge, this is the first report of genetic P. inflatum transformation via Agrobacterium. Unlike previous studies [13,15,28,29,37,43,44], the optimal conditions for single-copy T-DNA integration in P. inflatum were established. These conditions required a 24 h co-cultivation time with AS (200 µM) and 1 × 10 6 spores per 5 cm plate.…”

Section: Discussionmentioning

confidence: 99%

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Agrobacterium tumefaciens-Mediated Transformation of the Aquatic Fungus Phialemonium inflatum FBCC-F1546

Yoon,

Kim,

Kim

et al. 2023

JoF

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Phialemonium inflatum is a useful fungus known for its ability to mineralise lignin during primary metabolism and decompose polycyclic aromatic hydrocarbons (PAHs). However, no functional genetic analysis techniques have been developed yet for this fungus, specifically in terms of transformation. In this study, we applied an Agrobacterium tumefaciens-mediated transformation (ATMT) system to P. inflatum for a functional gene analysis. We generated 3689 transformants using the binary vector pSK1044, which carried either the hygromycin B phosphotransferase (hph) gene or the enhanced green fluorescent protein (eGFP) gene to label the transformants. A Southern blot analysis showed that the probability of a single copy of T-DNA insertion was approximately 50% when the co-cultivation of fungal spores and Agrobacterium tumefaciens cells was performed at 24–36 h, whereas at 48 h, it was approximately 35.5%. Therefore, when performing gene knockout using the ATMT system, the co-cultivation time was reduced to ≤36 h. The resulting transformants were mitotically stable, and a PCR analysis confirmed the genes’ integration into the transformant genome. Additionally, hph and eGFP gene expressions were confirmed via PCR amplification and fluorescence microscopy. This optimised transformation system will enable functional gene analyses to study genes of interest in P. inflatum.

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Section: Discussionmentioning

confidence: 98%

Section: Discussionmentioning

confidence: 99%

Agrobacterium tumefaciens-Mediated Transformation of the Aquatic Fungus Phialemonium inflatum FBCC-F1546

Yoon,

Kim,

Kim

et al. 2023

JoF

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“…Agrobacterium-mediated transformation results in good target gene expression, stable heredity, and low operational costs, and is the main application method in plant transgenic technology research [8][9][10]. However, some researchers still find that the differentiation and regeneration ability of resistant callus obtained by Agrobacterium infection would be reduced even if immature maize embryos are used as starting materials.…”

Section: Introductionmentioning

confidence: 99%

Optimization of Agrobacterium tumefaciens-Mediated Genetic Transformation of Maize

Liu¹,

Zhou²,

Liu³

et al. 2022

Phyton

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Immature embryos of inbred maize (Zea mays) lines (H8183, H8184, and H8185) were used for Agrobacterium infection. We used the β-glucuronidase gene (GUS) as the target gene and the glufosinate resistance gene (bar) as the selection marker. We conducted research on several aspects, such as different genotypes, coculture conditions, screening agent concentrations, and concentrations of indole-3-butytric acid (IBA), 6-benzylaminopurine (6-BA), and ascorbic acid (Vc) in the differentiation medium. We optimized the genetic transformation system, and the obtained results indicated that among the three lines studied, the induction rate of H8185 was the highest at 93.2%, followed by H8184, with H8183 having the lowest induction rate (80.1%). The best coculture method was that using the N6 coculture medium layered with a sterile filter paper. Using orthogonal analysis, we found that the optimal combination of the three factors in the differentiation medium was A 3 (1 mg mL −1 IBA), B 3 C 1 (1.6 mg mL −1 6-BA), and D 3 (1.5 mg mL −1 Vc). Through GUS staining analysis, Bar test-strip analysis, and polymerase chain reaction, five transgenic plants were finally obtained. This study established the optimal conditions for genetic transformation in maize.

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Infection of postharvest pear by Penicillium expansum is facilitated by the glycoside hydrolase (eglB) gene

Xu,

Godana,

et al. 2024

International Journal of Food Microbiology

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Agrobacterium tumefaciens-mediated transformation of Coniella granati

Cited by 5 publications

References 31 publications

Agrobacterium tumefaciens-Mediated Transformation of the Aquatic Fungus Phialemonium inflatum FBCC-F1546

Agrobacterium tumefaciens-Mediated Transformation of the Aquatic Fungus Phialemonium inflatum FBCC-F1546

Optimization of Agrobacterium tumefaciens-Mediated Genetic Transformation of Maize

Infection of postharvest pear by Penicillium expansum is facilitated by the glycoside hydrolase (eglB) gene

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