Agrobacterium tumefaciens‐mediated transformation and regeneration of herbicide resistant onion (<i>Allium cepa</i>) plants

Eady, Colin; Davis, Sheree; Farrant, Julie; Reader, J.K.; Kenel, Fernand

doi:10.1111/j.1744-7348.2003.tb00243.x

Cited by 33 publications

(31 citation statements)

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“…Nevertheless, this method is not fully effective and only have a minimal contribution towards purple blotch management. Genetic engineering could be an option towards purple blotch resistance in onion (Eady et al, 2003), but the industry and the consumers do not currently accept such plant varieties. In these circumstances, host resistance breeding could be the most effective way to control purple blotch disease.…”

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confidence: 99%

Identification of Novel Source of Resistance and Differential Response of Allium Genotypes to Purple Blotch Pathogen, Alternaria porri (Ellis) Ciferri

Nanda

Chand

Mandal

et al. 2016

The Plant Pathology Journal

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Purple blotch, caused by Alternaria porri (Ellis) Cifferi, is a serious disease incurring heavy yield losses in the bulb and seed crop of onion and garlic worldwide. There is an immediate need for identification of effective resistance sources for use in host resistance breeding. A total of 43 Allium genotypes were screened for purple blotch resistance under field conditions. Allium cepa accession ‘CBT-Ac77’ and cultivar ‘Arka Kalyan’ were observed to be highly resistant. In vitro inoculation of a selected set of genotypes with A. porri, revealed that 7 days after inoculation was suitable to observe the disease severity. In vitro screening of 43 genotypes for resistance to A. porri revealed two resistant lines. An additional 14 genotypes showed consistent moderate resistance in the field as well as in vitro evaluations. Among the related Allium species, A. schoenoprasum and A. roylei showed the least disease index and can be used for interspecific hybridization with cultivated onion. Differential reaction analysis of three A. porri isolates (Apo-Chiplima, Apn-Nasik, Apg-Guntur) in 43 genotypes revealed significant variation among the evaluated Allium species (P = 0.001). All together, the present study suggest that, the newly identified resistance sources can be used as potential donors for ongoing purple blotch resistance breeding program in India.

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confidence: 99%

Identification of Novel Source of Resistance and Differential Response of Allium Genotypes to Purple Blotch Pathogen, Alternaria porri (Ellis) Ciferri

Nanda

Chand

Mandal

et al. 2016

The Plant Pathology Journal

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“…We have not, however, been able to use this to investigate time courses through division after microfilament and microtubule disruption because dividing cells cannot be recognized before peroxisomal aggregation. Instead, we plan to investigate these time courses using cultures of stably transformed Allium (see Eady et al 2003) expressing peroxisome-targeted GFP to distinguish the roles of microtubules and microfilaments in peroxisomal aggregation.…”

Section: Collings and Harper-peroxisomal Aggregation During Cytokinesismentioning

confidence: 99%

Peroxisome Aggregation during Cytokinesis in Different Angiosperm Taxa

Collings¹,

Harper

2008

International Journal of Plant Sciences

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During cytokinesis in onion (Allium cepa L.) and leek (Allium porrum L.), all the peroxisomes present within the dividing cell aggregate in the phragmoplast adjacent to the developing cell plate. In order to understand the functional implications of this novel arrangement, especially in onion, which has hitherto been regarded as a model system in which to study plant cell division, we investigated how widespread the phenomenon was in selected monocots and dicots. During monocot cytokinesis, peroxisomes lacked aggregation in some taxa, notably grasses, and underwent partial aggregation in other taxa. However, complete aggregation of a cell's entire complement of peroxisomes was restricted to the genus Allium. Although peroxisomal aggregation has been suggested to function in the formation of the cell plate, the distribution of partial and complete aggregation in monocots did not match known differences in primary cell wall biochemistry. No aggregation was seen during cytokinesis in dicots. Through quantification of peroxisome distribution in cells whose actin microfilaments were disrupted with either latrunculin or cytochalasin, we demonstrated that peroxisomal aggregation is a microfilament-dependent process in Allium and the closely related plant Tristagma, which shows only partial aggregation. We speculate whether analysis of the peroxisomal proteome might reveal novel function(s) for aggregated peroxisomes during cytokinesis.

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“…Along with health and nutritional benefits associated with these compounds, these thiosulfides are also major contributors to the bitter taste of some onions [90] [92]. Three sets of transgenic onion plants containing antisense alliinase gene constructs (a CaMV 35S-driven antisense root alliinase gene, a CaMV 35S-driven antisense bulb alliinase, and a bulb alliinase promoter-driven antisense bulb alliinase) have been recently produced [93]. Results from the antisense bulb alliinase lines have been much more encouraging, and three lines were produced with barely detectable bulb alliinase levels and activity.…”

Section: Transgenic Vegetables and Nutritional Health Benefitsmentioning

confidence: 99%

Advances in Transgenic Vegetable and Fruit Breeding

Dias¹,

Ortíz²

2014

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Vegetables and fruits are grown worldwide and play an important role in human diets because they provide vitamins, minerals, dietary fiber, and phytochemicals. Vegetables and fruits are also associated with improvement of gastrointestinal health, good vision, and reduced risk of heart disease, stroke, chronic diseases such as diabetes, and some forms of cancer. Vegetable and fruit production suffers from many biotic stresses caused by pathogens, pests, and weeds and requires high amounts of plant protection products per hectare. United States vegetables farmers are benefiting from growing transgenic squash cultivars resistant to Zucchini yellow mosaic virus, Watermelon mosaic virus, and Cucumber mosaic virus, which were deregulated and commercialized since 1996. Bt-sweet corn has also proven effective for control of some lepidopteran species and continues to be accepted in the fresh market in the USA, and Bt-fresh-market sweet corn hybrids are released almost every year. Likewise, transgenic Bt-eggplant bred to reduce pesticide use is now grown by farmers in Bangladesh. Transgenic papaya cultivars carrying the coat-protein gene provide effective protection against Papaya ring spot virus elsewhere. The transgenic "Honey Sweet" plum cultivar provides an interesting germplasm source for Plum pox virus control. Enhanced host plant resistance to Xanthomonas campestris pv. musacearum, which causes the devastating banana Xanthomonas wilt in the Great Lakes Region of Africa, was achieved by plant genetic engineering. There are other vegetable and fruit crops in the pipeline that have been genetically modified to enhance their host plant resistance to insects and plant pathogens, to show herbicide tolerance, and to improve features such as slow ripening that extends the shelf-life of the produce. Consumers could benefit further from eating more nutritious transgenic vegetables and fruits. Transgenic plant breeding therefore provides genetically enhanced seed embedded technology that contributes to integrated pest management in horticulture by reducing pesticide sprays as well as improving food safety by minimizing pesticide residues. Furthermore, herbicide-tolerant transgenic crops can help reducing plough in fields, thereby saving fuel because of less tractor use, which also protects the structure of the soil by reducing its erosion. Transgenic vegetable and fruit crops could make important contributions to sustainable vegetable production and for more nu-J. S. Dias, R. Ortiz 1449 tritious and healthy food. Countries vary, however, in their market standards of acceptance of transgenic crops. Biotechnology products will be successful if clear advantages and safety are demonstrated to both growers and consumers.

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Agrobacterium tumefaciens‐mediated transformation and regeneration of herbicide resistant onion (Allium cepa) plants

Cited by 33 publications

References 9 publications

Identification of Novel Source of Resistance and Differential Response of Allium Genotypes to Purple Blotch Pathogen, Alternaria porri (Ellis) Ciferri

Identification of Novel Source of Resistance and Differential Response of Allium Genotypes to Purple Blotch Pathogen, Alternaria porri (Ellis) Ciferri

Peroxisome Aggregation during Cytokinesis in Different Angiosperm Taxa

Advances in Transgenic Vegetable and Fruit Breeding

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