Agrobacterium tumefaciens-Mediated Transformation

Frandsen, Rasmus John Normand

doi:10.1007/978-3-319-10142-2_14

Cited by 3 publications

(1 citation statement)

References 37 publications

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“…Reverse genetics is an effective means of investigating a potential virulence gene’s function by disrupting the target gene and analyzing the mutated strain [ 22 ], and loss of function has to be corroborated by the restoration of function via complementation. Among various fungal transformation techniques, Agrobacterium tumefaciens -mediated transformation (ATMT) has become a preferred transformation method for researchers because of its high conversion efficiency and transformant stability [ 23 ]. ATMT has been successfully applied to many fungi, including S. cerevisiae , T. mentagrophytes , and six ascomycetes and basidiomycetes species [ 24 , 25 , 26 , 27 , 28 ].…”

Section: Introductionmentioning

confidence: 99%

ZafA Gene Is Important for Trichophyton mentagrophytes Growth and Pathogenicity

Dai

Gao

et al. 2019

IJMS

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Trichophyton mentagrophytes is a common fungal pathogen that causes human and animal dermatophytosis. Previous studies have shown that zinc deficiency inhibits T. mentagrophytes growth, and the ZafA gene of T. mentagrophytes can code the functionally similar zinc finger transcriptional factor that can promote zinc ion absorption; however, the impact of ZafA on virulence and pathogenicity remains undetermined. To assess its gene function, the ZafA mutant, ZafA-hph, and the ZafA complemented strain, ZafA+bar, were constructed via Agrobacterium tumefaciens-mediated transformation. Polymerase chain reaction and Southern blot analyses were used to confirm the disruption. In vitro growth capacity and virulence analyses comparing ZafA-hph with wild-type T. mentagrophytes and ZafA+bar showed that ZafA-hph’s growth performance, reproduction ability, and zinc ion absorption capacity were significantly lower than the wild-type T. mentagrophytes and ZafA+bar. ZafA-hph also showed weak hair biodegradation ability and animal pathogenicity. Thus, the significant decrease in T. mentagrophytes’ growth ability and virulence was due to a lack of the zinc-responsive activity factor rather than the transformation process. This study confirmed that the T. mentagrophytes’ zinc-responsive activity factor plays important roles in the pathogen’s growth, reproduction, zinc ion absorption, and virulence. This factor is important and significant for effectively preventing and controlling T. mentagrophytes infections.

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Section: Introductionmentioning

confidence: 99%

ZafA Gene Is Important for Trichophyton mentagrophytes Growth and Pathogenicity

Dai

Gao

et al. 2019

IJMS

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Metabolic Engineering of Fungal Strains for Efficient Production of Cellulolytic Enzymes

Zhao

Zhang

et al. 2018

Fungal Cellulolytic Enzymes

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Agrobacterium tumefaciens-mediated genetic transformation of the Taxol-producing endophytic fungus Ozonium sp EFY21

Liu¹,

Wei²,

Zhou³

et al. 2013

Genet. Mol. Res.

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ABSTRACT. An efficient Agrobacterium tumefaciens-mediated genetic transformation method was successfully established for a newly isolated Taxol-producing fungus, Ozonium sp EFY21. A specific hygromycin B resistance expression vector, pCAMBIA1304'AN7-1, was constructed for fungal transformation. Key factors affecting transformation efficiency were thoroughly investigated and optimized. PCR amplification and Southern hybridization were used to verify the transformation events. This study should pave the way for future genetic modification studies of Ozonium sp EFY21.

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Agrobacterium tumefaciens-Mediated Transformation

Cited by 3 publications

References 37 publications

ZafA Gene Is Important for Trichophyton mentagrophytes Growth and Pathogenicity

ZafA Gene Is Important for Trichophyton mentagrophytes Growth and Pathogenicity

Metabolic Engineering of Fungal Strains for Efficient Production of Cellulolytic Enzymes

Agrobacterium tumefaciens-mediated genetic transformation of the Taxol-producing endophytic fungus Ozonium sp EFY21

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