Agrobacterium rhizogenes pRi8196 T-DNA: mapping and DNA sequence of functions involved in mannopine synthesis and hairy root differentiation.

Hansen, Geneviève; Larribe, Mireille; Vaubert, D.; Tempé, Jacques; Biermann, Brenda J.; Montoya, Alice L.; Chilton, Mary-Dell; Brevet, Jean

doi:10.1073/pnas.88.17.7763

Cited by 67 publications

(44 citation statements)

References 42 publications

(43 reference statements)

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“…11). rolB homologues of strains of A. rhizogenes other than the agropinetype strain also have coding regions longer than that of the NgrolB gene (28)(29)(30). Although the ORF of NgrolB starts at the corresponding base of RirolB, differences in bases at sites 633 and 723 bp from the initiation codon generate early-termination codons.…”

Section: Molecular Evolutionary Analysismentioning

confidence: 99%

Horizontal gene transfer and mutation: Ng rol genes in the genome of Nicotiana glauca

Aoki

Syōno²

1999

Proc. Natl. Acad. Sci. U.S.A.

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Section: Molecular Evolutionary Analysismentioning

confidence: 99%

Horizontal gene transfer and mutation: Ng rol genes in the genome of Nicotiana glauca

Aoki

Syōno²

1999

Proc. Natl. Acad. Sci. U.S.A.

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“…Nor do these bacteria express mannopine cyclase activity. The T region of one mannopine-type Ri plasmid contains close homologs of the mas1 and mas2 genes found on the T region of agropine-type Ri and Ti plasmids but lacks ags (13). Thus, the opine biosynthesis systems specified by the mannopine-and agropine-type T-DNAs differ only by the presence of ags.…”

Section: Figmentioning

confidence: 99%

A T-DNA gene required for agropine biosynthesis by transformed plants is functionally and evolutionarily related to a Ti plasmid gene required for catabolism of agropine by Agrobacterium strains

et al. 1997

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The mechanisms that ensure that Ti plasmid T-DNA genes encoding proteins involved in the biosynthesis of opines in crown gall tumors are always matched by Ti plasmid genes conferring the ability to catabolize that set of opines on the inducing Agrobacterium strains are unknown. The pathway for the biosynthesis of the opine agropine is thought to require an enzyme, mannopine cyclase, coded for by the ags gene located in the T R region of octopine-type Ti plasmids. Extracts prepared from agropine-type tumors contained an activity that cyclized mannopine to agropine. Tumor cells containing a T region in which ags was mutated lacked this activity and did not contain agropine. Expression of ags from the lac promoter conferred mannopine-lactonizing activity on Escherichia coli. Agrobacterium tumefaciens strains harboring an octopine-type Ti plasmid exhibit a similar activity which is not coded for by ags. Analysis of the DNA sequence of the gene encoding this activity, called agcA, showed it to be about 60% identical to T-DNA ags genes. Relatedness decreased abruptly in the 5 and 3 untranslated regions of the genes. ags is preceded by a promoter that functions only in the plant. Expression analysis showed that agcA also is preceded by its own promoter, which is active in the bacterium. Translation of agcA yielded a protein of about 45 kDa, consistent with the size predicted from the DNA sequence. Antibodies raised against the agcA product cross-reacted with the anabolic enzyme. These results indicate that the agropine system arose by a duplication of a progenitor gene, one copy of which became associated with the T-DNA and the other copy of which remained associated with the bacterium.Members of the genus Agrobacterium have evolved a unique interaction with certain plant species. Virulent agrobacteria harbor large extrachromosomal elements called Ti and Ri plasmids, a segment of which, the T region, is transferred from the bacterium to the susceptible plant during infections (6). Following transfer, this region, which then is called T-DNA, becomes integrated into the nuclear genome of the plant cell. Expression of hormone biosynthesis genes (onc) present on the integrated T-DNA results in the transformation of the normal plant cell into a crown gall tumor cell. The tumors characteristically produce novel low-molecular-weight metabolites called opines, whose synthesis also is directed by genes present on the T-DNA. Remarkably, although the T-DNA onc and opine biosynthesis (ops) genes are present on a bacterial replicon, they are expressed properly only in plant cells. Both gene classes lack identifiable bacterial promoters but contain 5Ј and 3Ј sequence motifs characteristic of plant transcriptional signals. In turn, the causative bacteria can utilize the opines produced by the tumors as a source of carbon, nitrogen, and energy (25, 28). Thus, Agrobacterium tumefaciens redirects plant cell metabolism to produce specific metabolites which the bacterium can use as growth substrates.This correlation between opine producti...

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“…It was found that the right T-DNA (T R -DNA) contains genes homologous to T-DNA of A. tumefaciens Ti plasmid (Huffman et al 1984;Jouanin 1984;Vilaine and CasseDelbart 1987;Hansen et al 1991;Chandra 2012). Among them, the most important genes are those homologous to the tms1 and tms2 of the Ti-plasmid.…”

Section: T R -Dnamentioning

confidence: 99%

“…Since these strains are still capable to induce a "hairy-root" phenotype, it can be said that the presence of the aux genes on T R -DNA is not necessary to generate hairy root phenotype. It has been demonstrated that the aux genes are required to support the "hairy root" phenotype and to extend the host range of the bacterium Cardarelli et al 1987b;Hansen et al 1991;Sevon and OksmanCaldentey 2002).…”

Section: T R -Dnamentioning

confidence: 99%

Agrobacterium rhizogenes-Mediated Transformation and Its Biotechnological Applications in Crops

2013

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The history of Agrobacterium-related plant biotechnology goes back for more than three decades with the discovery of molecular mechanisms of crown gall disease in plants. After 1980s, gene technologies began developing rapidly and today, related with the improved gene transfer methods, plant biotechnology has become one of the most important branches in science. Till now, the most important genes related with agricultural affairs have been utilized for cloning of plants with the deployment of different techniques used in genetic engineering. Especially, Agrobacterium tumefaciens was used extensively for transferring desired genetic materials to plants rapidly and effectively by the researchers to create transgenic plants. Recognition of the biology of Agrobacterium species and newly developed applications of their T-DNA systems has been a great step in plant biotechnology. This chapter provides the reader with extensive information on A. rhizogenes which is responsible for the development of hairy root disease in a wide range of dicotyledonous plants and its T-DNA system. This knowledge will be useful in improving utilization of crops and the formulation of new and up-graded transgenic based food products.

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Agrobacterium rhizogenes pRi8196 T-DNA: mapping and DNA sequence of functions involved in mannopine synthesis and hairy root differentiation.

Cited by 67 publications

References 42 publications

Horizontal gene transfer and mutation: Ng rol genes in the genome of Nicotiana glauca

Horizontal gene transfer and mutation: Ng rol genes in the genome of Nicotiana glauca

A T-DNA gene required for agropine biosynthesis by transformed plants is functionally and evolutionarily related to a Ti plasmid gene required for catabolism of agropine by Agrobacterium strains

Agrobacterium rhizogenes-Mediated Transformation and Its Biotechnological Applications in Crops

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