Agrobacterium rhizogenes influences aervine enhancement in hairy root culture of Aerva javanica (Burm.f.) Juss. ex Schult and in silico assessment of human breast cancer activity

Abstract

A highly effective Agrobacterium-mediated transformation approach was availed to enhance the production of bioactive metabolites in Aerva javanica. This study reported that callus-based transformation exhibited high efficiency and best reproducibility. The optimized hairy root inducing media devoid of plant growth regulators and strain ATCC15834 induced the hairy root (69.67%) from callus with multiple roots and high efficiency. The factors influencing the rate of callus transformation are as follows; different strains of Agrobacterium rhizogenes (ATCC 15834, MTCC 2364, MTCC 532, R1000, and LBA 9204), cocultivation time of 2 days, and infection time period of 20 min. The stable transformation by the influence of rol genes was confirmed by polymerase chain reaction. The ultra-performance liquid chromatographic analysis quantified the yield of aervine (81.41 ± 2.82 µg/mL) in aervine enriched chloroform hairy root extract from in vitro callus (AECHRIC). Initial in silico analysis for aervine on breast cancer target proteins produced low-binding energy against PR (−8.1 kcal/mol) and AECHRIC showed the better cytotoxic effect on MCF-7 (149.86 ± 0.32 µg/mL) cell line. Due to the dramatic effect of AECHRIC, it could be considered as a potent anticancer drug in the pharmaceutical hub.

Optimization of factors affecting Agrobacterium-mediated hairy root induction in Vitex negundo L. (Lamiaceae)

“…In corroboration to our result, Giri et al (2001) as well as Brijwal and Tamta, (2015) also suggested about the 48 hours of co-cultivation duration as beneficial for hairy root induction for Artemisia annua and Berberis aristate respectively. Further, 48 hours of co-cultivation time was suggested by Srinivasan et al (2023) for hairy root development in Aerva javanica by five different bacterial strains (ATCC 15834, R1000, LBA 9204, MTCC 2364, and MTCC 532).…”