Agrobacterium -mediated transformation of niger [ Guizotia abyssinica (L. f.) Cass.] using seedling explants

Murthy, Hosakatte Niranjana; Jeong, Jang Hae; Choi, Yong Eui; Paek, Kee-Yoeup

doi:10.1007/s00299-003-0573-1

Cited by 10 publications

(3 citation statements)

References 10 publications

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“…In addition, there was not any other information on shoot propagation from Impatiensʼ hypocotyls (HUYOP et al, 2009). Our results have confirmed previous reports of Arabis gunnisoniana and nyger (Guazotia abysinica) (MURTHY et al, 2003). Remarkable differences were observed in shoot regeneration with BAP compared to other cytokines sources in Impatiens platypetala Lind, Impatiens L. interspesific hybrids (KYUNGKUL and STEPHENS, 1987).…”

Section: Fig 3 Effect Of Hormones On Survival Rate In Impatiens Wallerianasupporting

confidence: 92%

Evaluation of different growth hormones mediated callus induction and regeneration as well as the effect of colchicines, ethyl methanesulfonate (EMS) and gamma radiation on some traits of Impatiens walleriana

et al. 2021

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Impatiens is an ornamental member of family Balsaminaceae. This plant mostly propagated by vegetative technique, which generally time wasting process. It is often multiplicities via seed but is barricaded by F1 seed sterility. In vitro culture of Impatiens walleriana has much significant function in fast proliferation with useful features and elicitation of healthful and disease-free plants. This experiment was conducted to investigate the impact of medium and different hormones on in vitro propagation of Impatiens walleriana by using a completely randomized design. MS medium was prepared along with various concentrations of BAP, TDZ and ZEA. Callus was induced and grew well in media supplemented with 0.5 mg/l NAA + 1 mg/l BAP. In order to indirect propagation, explants were cultured in same media containing BAP, ZEA and TDZ in combination with NAA. These treatments have ability to organogenesis. The results revealed that the control treatment had the lowest effect on traits including shoot percentage, number of shoots, number of leaves, shoot length, fresh and dry weight, and it lead to maximum proliferations in medium supplemented with 0.5 mg/l NAA + 1 mg/l BAP. The highest root length and rooting percentage was observed in 0.5 mg/l IBA + 0.5 mg/l BAP. In addition, the effect of mutation agents was studied. Aseptic samples were treated with ?- irradiation, Ethyl Methane Sulfonate and colchicines at growth chamber. Treatments with 30 and 60 grey respectively had the lower survival rate, growth rate and polyploidy while colchicines with 0.1 and 0.2 had the highest rats. Regarding to these, the present technique illustrate an effective system for in vitro reproduction of Impatiens walleriana by hypocotyls cultures. In addition, colchicines proved to be effective in induction of polyploidy in this plantlet.

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Section: Fig 3 Effect Of Hormones On Survival Rate In Impatiens Wallerianasupporting

confidence: 92%

Evaluation of different growth hormones mediated callus induction and regeneration as well as the effect of colchicines, ethyl methanesulfonate (EMS) and gamma radiation on some traits of Impatiens walleriana

et al. 2021

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“…Different explants of J. curcas have been reported to be used in transformation (Kajikawa et al 2012 ; Khemkladngoen et al 2011 ; Kumar et al 2010 ; Li et al 2008 ; Mao et al 2009 ; Misra et al 2012 ; Pan et al 2010 ). In many plants, cotyledon explants are better suited to regeneration and transformation than other explants (Li et al 2008 , 2006 ; Murthy et al 2003 ). Cotyledons excised from mature seeds of J. curcas , which are available in large quantities for transformation year-round, were found susceptible to A. tumefaciens infection (Li et al 2014 ; Pan et al 2010 ; Tao et al 2015 ).…”

Section: Discussionmentioning

confidence: 99%

An efficient protocol for Agrobacterium-mediated transformation of the biofuel plant Jatropha curcas by optimizing kanamycin concentration and duration of delayed selection

Tang

et al. 2015

Plant Biotechnol Rep

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Jatropha curcas is considered a potential biodiesel feedstock crop. Currently, the value of J. curcas is limited because its seed yield is generally low. Transgenic modification is a promising approach to improve the seed yield of J. curcas. Although Agrobacterium-mediated genetic transformation of J. curcas has been pursued for several years, the transformation efficiency remains unsatisfying. Therefore, a highly efficient and simple Agrobacterium-mediated genetic transformation method for J. curcas should be developed. We examined and optimized several key factors that affect genetic transformation of J. curcas in this study. The results showed that the EHA105 strain was superior to the other three Agrobacterium tumefaciens strains for infecting J. curcas cotyledons, and the supplementation of 100 mM acetosyringone slightly increased the transient transformation frequency. Use of the appropriate inoculation method, optimal kanamycin concentration and appropriate duration of delayed selection also improved the efficiency of stable genetic transformation of J. curcas. The percentage of β-glucuronidase positive J. curcas shoots reached as high as 56.0 %, and 1.70 transformants per explant were obtained with this protocol. Furthermore, we optimized the root-inducing medium to achieve a rooting rate of 84.9 %. Stable integration of the T-DNA into the genomes of putative transgenic lines was confirmed by PCR and Southern blot analysis. Using this improved protocol, a large number of transgenic J. curcas plantlets can be routinely obtained within approximately 4 months. The detailed information provided here for each step of J. curcas transformation should enable successful implementation of this transgenic technology in other laboratories.Electronic supplementary materialThe online version of this article (doi:10.1007/s11816-015-0377-0) contains supplementary material, which is available to authorized users.

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“…Genetic engineering is providing a valuable means of expanding the floriculture gene pool, so promoting the generation of new commercial varieties. Nevertheless genetic engineering is based on plant regeneration or micropropagation and genetic transformation (Murthy et al 2003). To our knowledge, genetic transformation of cockscomb has not reported up to date.…”

Section: Introductionmentioning

confidence: 99%

Transformation of PttKN1 gene to cockscomb

et al. 2009

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We have established a shoot regeneration system and genetic transformation of cockscomb (Celosia cristata and Celosia plumosus). The best results in terms of frequency of shoot regeneration and number of shoot buds per explant are observed on media supplemented with 0.5 mg l -1 6-BA (for explants of apical meristems of C. cristata) or 2.0 mg l -1 6-BA, 0.5 mg l -1 NAA and 0.5 mg l -1 IAA (for hypocotyls explants of C. plumosus). We use apical meristems of C. cristata and hypocotyls of C. plumosus as the starting material for transformation. A novel KNOTTED1-like homeobox1 (KNOX), PttKN1 (Populus tremula 9 P. tremuoides knotted1) isolated from the vascular cambial region of hybrid aspen, is introduced into cockscomb by Agrobacterium. A series of novel phenotypes are obtained from the transgenic cockscomb plants, including lobed or rumpled leaves, partite leaves and two or three leaves developed on the same petiole, on the basis of their leaf phenotypes. Transformants are selected by different concentrations of kanamycin. Transformants are confirmed by PCR of the NptII gene and PCR or RT-PCR of PttKN1 gene. Furthermore, RT-PCR shows that 35S:: PttKN1 RNA levels do not correlate with phenotypic severity. It is discussed that our results bring elements on possible function of PttKN1 gene. To our knowledge, genetic transformation of cockscomb is first reported.

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Agrobacterium -mediated transformation of niger [ Guizotia abyssinica (L. f.) Cass.] using seedling explants

Cited by 10 publications

References 10 publications

Evaluation of different growth hormones mediated callus induction and regeneration as well as the effect of colchicines, ethyl methanesulfonate (EMS) and gamma radiation on some traits of Impatiens walleriana

Evaluation of different growth hormones mediated callus induction and regeneration as well as the effect of colchicines, ethyl methanesulfonate (EMS) and gamma radiation on some traits of Impatiens walleriana

An efficient protocol for Agrobacterium-mediated transformation of the biofuel plant Jatropha curcas by optimizing kanamycin concentration and duration of delayed selection

Transformation of PttKN1 gene to cockscomb

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