Agrobacterium-mediated transformation of Musa acuminata cv. “Grand Nain” scalps by vacuum infiltration

Acereto-Escoffié, Pablo; Chí-Manzanero, Bartolomé; Echeverría-Echeverría, S.; Grijalva, R.; Kay, Andrew-James; González-Estrada, Tomas; Castaño, Enrique; Rodríguez-Zapata, Luis Carlos

doi:10.1016/j.scienta.2005.01.028

Cited by 28 publications

(16 citation statements)

References 11 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…"Grand Nain" scalps (Acereto-Escoffié et al 2005) and Argemone mexicana L. (Godoy-Hernández et al 2008), did not show endogenous GUS-like activity in evaluated tissues.…”

Section: Discussionmentioning

confidence: 96%

Endogenous GUS-like activity in Capsicum chinense Jacq.

Solís-Ramos¹,

González-Estrada²,

Andrade‐Torres³

et al. 2010

Electron. J. Biotechnol.

View full text Add to dashboard Cite

show abstract

“…"Grand Nain" scalps (Acereto-Escoffié et al 2005) and Argemone mexicana L. (Godoy-Hernández et al 2008), did not show endogenous GUS-like activity in evaluated tissues.…”

Section: Discussionmentioning

confidence: 96%

Endogenous GUS-like activity in Capsicum chinense Jacq.

Solís-Ramos¹,

González-Estrada²,

Andrade‐Torres³

et al. 2010

Electron. J. Biotechnol.

View full text Add to dashboard Cite

show abstract

“…The determination of histology and origin of callus tissue is considered important in the isolation of mutants and nonchimeric plants. The findings of callus formation may prove useful in the improvement of this crop plant through genetic modification at the cellular level since there have been several reports on genetic transformation in banana (Acereto-Escoffié et al, 2005;Khanna et al, 2004;Sreeramanan et al, 2006;Tripathi et al, 2007). However, it cannot be determined from the present study whether callus proliferation was of a single or multicellular origin.…”

Section: Histological Analysesmentioning

confidence: 99%

Untitled

Kanchanapoom

KORAPATCHAIKUL

2012

Not Sci Biol

View full text Add to dashboard Cite

Yellow compact calluses were induced from in vitro-grown shoot tips of diploid bananas (Musa acuminata, AA group) 'Kluai Sa' and 'Kluai Leb Mu Nang' on a modified Murashige and Skoog (MS) medium containing 100 mg/L malt extract, 50 mg/L proline, 50 mg/L cysteine, 100 mg/L glutamine, 1 mg/L biotin, 7 mg/L Dicamba and 2 mg/L TDZ. Green shoot buds were induced after transfer of the yellow compact calluses to the same MS medium but supplemented with 1 mg/L NAA and 3 mg/L BA and plant regeneration was achieved through organogenesis in callus cultures. Regenerated shoots were rooted on MS medium containing 0.2% activated charcoal but without plant growth regulators. Histological analysis revealed that calluses originated from small dense cells with well stained cytoplasm and nucleus typical of meristematic cells.

show abstract

“…One is the use of vacuum infiltration before co-culturing of the explants with bacteria (Charity et al 2002;Acereto-Escoffié et al 2005); another one is causing micro-wounds (e.g. biobalistics particles) to the explants prior to co-culture with bacteria (Xue et al 2006;Valdez-Ortiz et al 2007).…”

Section: Kanamycin For Transformed Plant Cells Selectionmentioning

confidence: 99%

Transient genetic transformation of embryogenic callus of Cocos nucifera

et al. 2011

View full text Add to dashboard Cite

Coconut palm (Cocos nucifera) is a plant species recalcitrant to in vitro morphogenesis and no protocols for the genetic transformation of coconut tissues have been published. The present study aimed to develop a protocol for genetic transformation of this palm species; evaluating reporter genes, transformation methods, and conditions for the use of antibiotics to select transformed plant cells. The gene gusA was first used for Agrobacterium tumefaciens mediated transformation of coconut embryogenic calli. However, endogenous GUS-like activity was found in calli not co-cultured with bacteria. Then essays for Agrobacterium-mediated transformation were developed using green and red fluorescent genes. Both genes are suitable as reporter genes for coconut transformation. In order to establish a protocol for coconut genetic transformation, an approach was used that combined biobalistics to generate micro-wounds in explants, vacuum infiltration and co-culture with Agrobacterium tumefaciens (C58C1 + pER10W-35SRed containing the embryogenesis related gene WUSCHEL). Calli treated with the combined protocol showed red fluorescence with greater intensity and greater area than calli treated with either biobalistics or infiltration, followed by bacteria co-culture. PCR amplification of DNA extracts from transformed embryogenic callus produced a band with the expected size using WUSCHEL primers (862 bp). No band was obtained using the VirE2 primers. This is the first report of transient genetic transformation of C. nucifera and it is the first step toward a protocol that will be useful for the study of the role of genes of interest and for practical applications, such as the improvement of coconut micropropagation via somatic embryogenesis.

show abstract

Agrobacterium-mediated transformation of Musa acuminata cv. “Grand Nain” scalps by vacuum infiltration

Cited by 28 publications

References 11 publications

Endogenous GUS-like activity in Capsicum chinense Jacq.

Endogenous GUS-like activity in Capsicum chinense Jacq.

Untitled

Transient genetic transformation of embryogenic callus of Cocos nucifera

Contact Info

Product

Resources

About